Retention characteristics of aliphatic compounds on cellulose acetates as a stationary phase with an aqueous mobile phase

1989 ◽  
Vol 28 (5-6) ◽  
pp. 279-284 ◽  
Author(s):  
Y. Kiso ◽  
T. Kitao ◽  
Ge Yong-Sheng ◽  
K. Jinno
Keyword(s):  
Stationary Phase ◽  
Mobile Phase ◽  
Aliphatic Compounds ◽  
Cellulose Acetates ◽  
Retention Characteristics

A Study on the variation of Apigenin content in Cardiospermum halicacabum from 21 districts of Tamil Nadu by HPTLC

2021 ◽  
pp. 2613-2618
Author(s):  
A Rajasekaran ◽  
V Abirami ◽  
S Arunkumar ◽  
Priya G Bharani ◽  
S Gugapriya ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Stationary Phase ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
Tamil Nadu ◽  
Leaf Extract ◽  
Major Constituent ◽  
Marker Compound ◽  
Aluminium Sheets ◽  
Hptlc Method

Cardiospermum halicacabum is one of the most potent medicinal plants used in Indian traditional systems of medicine for the treatment of various diseases, mainly for arthritis. Apigenin is one of the major constituent present in Cardiospermum halicacabum. The present study mainly aimed to estimate the content of major constituent apigenin present in Cardiospermum halicacabum collected from 21 districts of Tamil Nadu by HPTLC method using the marker compound apigenin. The HPTLC method was performed using HPTLC aluminium sheets precoated with Silica Gel 60 GF254 as stationary phase and Toluene: Ethyl acetate: formic acid: methanol (3:6:1.6:0.4 v/v) as the mobile phase. The developed chromatogram was scanned at 254nm using Camag Scanner III. The Rf value of standard apigenin and apigenin in the leaf extract of Cardiospermum halicacabum was found to be in the range of 0.80 to 0.89. Plant collected from Cuddalore district of Tamil Nadu was found to contain relatively high amount of marker compound apigenin than other regions.

scholarly journals HPLC FINGERPRINT OF ORGANIC ACIDS IN FRUIT JUICES

1970 ◽  
Vol 62 ◽  
Author(s):  
Loredana Leopold ◽  
Diehl Horst ◽  
Carmen Socaciu
Keyword(s):  
Organic Acids ◽  
Stationary Phase ◽  
Organic Acid ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Fruit Juices ◽  
Hplc Fingerprint ◽  
Organic Acid Profile ◽  
Phase Column ◽  
Reversed Phase Column

Organic acids give fruit products their characteristic tartness and vary in combination and in concentrations among different juices. The organic acid profile can be used to identify a juice or verify its purity. Typically, organic acids in fruit juices are identified and quantified by using methods such as HPLC. In this procedure, reversed phase column is used to separate and identificate six organic acids. Because several of the analytes are extremely difficult to resolve, a aqueous mobile phase is needed to enhance interaction between the acids and the C18 stationary phase.

scholarly journals Analysis of Carotenoid Pigment Types in the Carapace of the Male Crab Grapsus albolinetaus Latreille in Milbert 1812

2021 ◽  
Vol 9 (1) ◽  
pp. 132
Author(s):  
Fatika Sari Mokoginta ◽  
Darus Saadah Johanis Paransa ◽  
Kurnati Kemer ◽  
James J. H. Paulus ◽  
Nickson J. Kawung ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Column Chromatography ◽  
Metabolic Pathways ◽  
Mobile Phase ◽  
Separation Technique ◽  
Carotenoid Pigments ◽  
Carotenoid Pigment ◽  
Silica Powder ◽  
Male Crab ◽  
Yellow Orange

Carotenoid pigments have various colors such as yellow, orange, or red-orange. One of the carotenoid pigment sources is Crab G. albolineatus Latreille in Milbert 1812. Column chromatography separation technique was used to determine the metabolism of carotenoid pigments in the crab G. albolineatus latreille in Milbert 1812. This CC separation used hexane and acetone as the developer solution (70:30). The developer solution as known as the mobile phase is semipolar, while the stationary phase is silica powder G60. Therefore, it formed two metabolic pathways. The male G. albolineatus crab used in this study was on the D3 molting stage which had a concentration of 36.37 g/g dry residue and 4.72 g content. The types of pigments identified are: β – karoten, Zeaxanthin, lutein, β – kriptoxanthin dan Astaxanthin.Keywords: Carotenoid Pigments; G. albolineatus; Column Chromatography; MoltingAbstrakPigmen karotenoid memiliki berbagai warna seperti kuning, oranye, atau merah oranye. Salah satu sumber pigmen karotenoid adalah pada karapas kepiting G. albolineatus Latreille in Milbert 1812. Untuk mengetahui metabolisme jenis pigmen karotenoid pada kepiting Grapsus albolineatus latreille in Milbert 1812 yaitu menggunakan pemisahan kromatografi Kolom. Pemisahan KK ini menggunakan larutan pengembang heksan dan aseton (70:30). Larutan pengembang merupakan fase gerak yang bersifat semipolar dan fase diamnya menggunakan bubuk silika G60. Terbentuk dua  jalur metabolisme. Kepiting G. albolineatus jantan yang digunakan pada penelitian berada di stadium molting D3 dengan konsentrasi sebesar 36,37 µg/g berat residu kering dan kandungan 4,72 µg. Jenis pigmen yang teridentifikasi yaitu : β – karoten, Zeaxanthin, lutein, β – kriptoxanthin dan Astaxanthin.Kata kunci: Pigmen; Karotenoid; G. albolineatus; Kromatofrafi Kolom; Molting

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