Gas chromatography in qualitative analysis Part 9. The prediction of retention data in gas liquid chromatography by means of me values

1969 ◽  
Vol 2 (9) ◽  
pp. 397-400 ◽  
Author(s):  
M. B. Evans
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Qualitative Analysis ◽  
Gas Liquid Chromatography ◽  
Retention Data ◽  
Prediction Of Retention

DETERMINATION OF PLASMA OESTRIOL IN NORMAL PREGNANCY AND LABOUR USING GAS-LIQUID CHROMATOGRAPHY

1971 ◽  
Vol 51 (3) ◽  
pp. 447-454 ◽  
Author(s):  
W. COOPER ◽  
M. G. COYLE ◽  
J. A. MILLS
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Internal Standard ◽  
Normal Pregnancy ◽  
Gas Liquid Chromatography ◽  
Chemical Purification ◽  
Peripheral Plasma ◽  
Pregnancy And Delivery

SUMMARY A method is described for estimating oestriol in 2–10 ml samples of human pregnancy peripheral plasma. It incorporates acid hydrolysis, chemical purification, methylation, chromatography on alumina columns, formation of a derivative and quantitative determination by gas chromatography. A radioactive internal standard was added to correct for procedural losses. Plasma oestriol determinations in five normal patients throughout pregnancy and delivery are reported.

Quantitation of anticonvulsant drugs in serum by gas-chromatography on the stationary phase SP-2510.

1978 ◽  
Vol 24 (3) ◽  
pp. 483-485 ◽  
Author(s):  
W Godolphin ◽  
J Thoma
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
Serum Cholesterol ◽  
Anticonvulsant Drugs ◽  
Gas Liquid Chromatography ◽  
Column Packing ◽  
Extraction Procedures

Abstract A new column packing, SP-2510 DA (Supelco, Inc., Bellefonte, Pa. 16823), is an excellent stationary phase for the determination of a wide variety of anticonvulsant drugs by gas--liquid chromatography without derivatization. However, when uncomplicated extraction procedures are used, serum cholesterol interferes with the determination of primidone. By the simple expedient of adding a short "pre-column" containing another phase (SP-2250 DA) the problem is overcome.

Monitoring drug concentrations in a case of combined overdosage with primidone and methsuximide.

1976 ◽  
Vol 22 (6) ◽  
pp. 915-921 ◽  
Author(s):  
G F Johnson ◽  
C J Least ◽  
J W Serum ◽  
E B Solow ◽  
H M Solomon
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Therapeutic Decision ◽  
Serum Samples ◽  
Monitoring Drug ◽  
Drug Concentrations

Abstract We describe a case of fatal overdosage with primidone and methsuximide. During the early phase of the patient's hospital course we found concentrations of methsuximide, N-desmethylmethsuximide, and primidone in serum that far exceeded the usual therapeutic concentrations, as determined by gas-liquid chromatography. Determination of N-desmethylmethsuximide in peritoneal fluid demonstrated concentrations comparable to those in serum. This led to the therapeutic decision to manage the patient by dialysis. Subsequently, serum samples collected during the course of hospitalization were analyzed quantitatively by gas-liquid chromatography for methsuximide, N-desmethylmethsuximide, primidone, phenobarbital, and diphenylhydantoin. Selected serum specimens were also analyzed by gas chromatography-mass spectrometry, and N-methyl-2-hydroxymethyl-2-phenylsuccinimide, a metabolite of methsuximide not previously described in human serum, was identified by analysis of its mass spectrum.

Concentration of Glutethimide and Associated Compounds in Human Serum and Cerebrospinal Fluid After Drug Overdose

1974 ◽  
Vol 20 (2) ◽  
pp. 195-199 ◽  
Author(s):  
Martin Gold ◽  
Ernest Tassoni ◽  
Michael Etzl ◽  
George Mathew
Keyword(s):  
Gas Chromatography ◽  
Cerebrospinal Fluid ◽  
Liquid Chromatography ◽  
Serum Concentration ◽  
Human Serum ◽  
Drug Overdose ◽  
Gas Liquid Chromatography ◽  
Maximum Serum ◽  
Maximum Serum Concentration ◽  
Maximal Serum Concentration

Abstract Serum and cerebrospinal fluid of patients in coma owing to glutethimide overdose was assayed for glutethimide and associated compounds. The sample was extracted with chloroform and the extract assayed by gas-liquid chromatography on a column of "3% OV-17." Up to six constituents were found in serum in addition to glutethimide, only three of which were ever present in substantial quantity. The peaks seen on gas chromatography were numbered in order of elution. Glutethimide was peak No. 2. Peak No. 1 usually reached a maximum serum concentration in 10 or less hours, as did peak No. 4. Peak No. 3 reached a maximal serum concentration in 10 h or sooner also, but at 20 h the amount had changed little in most patients. Ambre and Fischer [Res. Commun. Pathol. Pharmacol. 4, 307 (1972)] speculate that peak No. 3 plays an important role in maintaining coma. Neither the pattern of change nor the relative concentrations in serum or cerebrospinal fluid on waking support their hypothesis.

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