HPLC determination of nicorandyl in the rat blood serum

1996 ◽  
Vol 30 (2) ◽  
pp. 130-131 ◽  
Author(s):  
O. I. Kal'chenko ◽  
L. M. Zaitsev ◽  
B. M. Klebanov ◽  
I. I. Krasavtsev ◽  
Yu. V. Korotkii ◽  
...  
Keyword(s):  
Blood Serum ◽  
Hplc Determination ◽  
Rat Blood

scholarly journals Rat Serum Carboxylesterase Partly Hydrolyses Gamma-Butyrobetaine Esters

2009 ◽  
Vol 60 (2) ◽  
pp. 147-156 ◽  
Author(s):  
Lida Bagdonienė ◽  
Danutė Labeikytė ◽  
Ivars Kalviņš ◽  
Veronika Borutinskaitė ◽  
Aleksandrs Prokofjevs ◽  
...  
Keyword(s):  
Blood Serum ◽  
Esterase Activity ◽  
Colorimetric Determination ◽  
Reaction Products ◽  
Rat Serum ◽  
Rat Blood ◽  
Optical Absorbance ◽  
Hydrolysis Of ◽  
Related Compounds

Rat Serum Carboxylesterase Partly Hydrolyses Gamma-Butyrobetaine EstersAlthough described some time ago, gamma-butyrobetaine esters and related compounds have not gained much attention from researchers, and their physiological function remains obscure. Formerly we detected GBB-esterase enzymatic activity in rat blood serum using phenylated gamma-butyrobetaine as an artificial substrate of the enzyme and HPLC. The aim of the present work was to develop an assay that would enable spectrophotometric or colorimetric determination of the reaction products of GBB-esterase activity and to reveal individual proteins performing GBB-esterase activity in rat blood serum. For this purpose gamma-butyrobetaine 1-naphthyl ester was synthesised. Hydrolysis of this ester releases 1-naphthol, which increases the optical absorbance at 322 nm. We have shown that the enzymatic hydrolysis of GBB 1-naphthyl ester to 1-naphthol in rat blood serum is due to GBB-esterase activity. An attempt was done to purify the enzyme from rat blood serum. By combining DEAE Sepharose at pH 4.2 and affinity chromatography with procainamide we achieved a 68-fold enrichment of GBB-esterase activity in our preparations. Separation of fraction proteins in 2D protein electrophoresis with following mass-spectrometry indicated that GBB esterase activity in rat blood serum is performed in part by carboxylesterase.

scholarly journals Development of Simultaneous Determination Method of a Pregnan Steroid with Gestagenic Activity – Gestobutanoil and Two its Metabolites in Rat Serum

2021 ◽  
Vol 10 (2) ◽  
pp. 112-118
Author(s):  
E. S. Stepanova ◽  
L. M. Makarenkova ◽  
S. V. Goryainov ◽  
T. A. Fedotcheva ◽  
N. L. Shimanovsky
Keyword(s):  
Blood Serum ◽  
Simultaneous Determination ◽  
Megestrol Acetate ◽  
Reaction Products ◽  
Active Metabolites ◽  
Rat Serum ◽  
Rat Blood ◽  
Esi Ms ◽  
Pharmacologically Active

Introduction. Gestobutanoil is a synthetic pregnane steroid with gestagenic activity. Gestobutanoil has two pharmacologically active metabolites (AMOL and megestrol acetate). This implies the need for a detailed study of the kinetics of metabolites. It is rational to combine the study of the pharmacokinetics of gestobutanoil and its metabolites (AMOL and megestrol acetate). The simultaneous determination of several analytes in the rats’ serum can be carried out using chromatography-mass-spectrometry.Aim. Development of an analytical method for the simultaneous determination of gestobutanoil and two its metabolites in a biomatrix (rat serum).Materials and methods. The following methods were used to determine gestobutanoyl and two its metabolites in a biological matrix: GC-MS, HPLCESI-MS, HPLC-ESI-MS with derivatization, HPLC-APCI-MS.Results and discussion. When working with GC-MS, the chromatographic peaks of gestobutanoyl, AMOL, and megestrol acetate were strongly blurred and superimposed on each other, which is apparently due to the thermolability of the substances. The GC-MS method was abandoned in favor of HPLC. Analytes were separated by HPLC gradient elution on a C18 column. ESI ionization did not give typical protonated ions of gestobutanoyl and AMOL, and the intense signals of their cationized ions and fragment ions, which were observed in the spectra of AMOL and gestobutanoyl, could not ensure the reproducibility of the spectra, since the conditions of their formation are not suitable for routine analysis. Derivatization of analytes to form oximes and substituted hydrazones did not give the expected reaction products for HPLC-ESI-MS. APCI made it possible to remove intense cationized ions from the spectra of gestobutanoyl and AMOL and to increase the reliability of the method. The HPLCAPCI-MS technique was reproduced on model rat blood serum.Conclusion. An HPLC-MS method was developed for the simultaneous determination of gestobutanoyl, megestrol acetate, and AMOL. The technique was tested on a model rat blood serum containing all three analytes.

Determination of FD&C Red No. 3 (Erythrosine) in Rat Blood Serum

1973 ◽  
Vol 56 (6) ◽  
pp. 1458-1459
Author(s):  
Manjeet Singh ◽  
Charles Graichen
Keyword(s):  
Blood Serum ◽  
Extraction Procedure ◽  
Rat Blood ◽  
Main Component

Abstract The extraction procedure used to isolate trace amounts of FD&C Red No. 3 (erythrosine) in rat blood serum is described. The blood sera from rats on diets containing FD&C Red No. 3 contained iodofluoresceins in such quantities that protein-bound iodine tests had no meaning. The lower iodinated subsidiary colors in FD&C Red No. 3 are absorbed and retained in the blood of rats to a greater degree than is tetraiodofluorescein, the main component of this color.

HPLC determination of pefloxacin and ciprofloxacin in blood serum

1999 ◽  
Vol 33 (4) ◽  
pp. 219-221 ◽  
Author(s):  
G. É. Brkich ◽  
A. P. Arzamastsev ◽  
É. M. Kaz'mina ◽  
A. V. Mikhalev
Keyword(s):  
Blood Serum ◽  
Hplc Determination

RP-HPLC determination of paraoxonase 3 activity in human blood serum

2006 ◽  
Vol 42 (1) ◽  
pp. 113-119 ◽  
Author(s):  
Zofia Suchocka ◽  
Joanna Swatowska ◽  
Jan Pachecka ◽  
Piotr Suchocki
Keyword(s):  
Blood Serum ◽  
Human Blood ◽  
Human Blood Serum ◽  
Hplc Determination ◽  
Rp Hplc

HPLC Determination of Ochratoxin A in a Low Volume of Human Blood Serum

2008 ◽  
Vol 41 (5) ◽  
pp. 757-766 ◽  
Author(s):  
R. Ghali ◽  
K. Hmaissia‐Khlifa ◽  
Ch. Mezigh ◽  
H. Ghorbel ◽  
K. Maaroufi ◽  
...  
Keyword(s):  
Blood Serum ◽  
Ochratoxin A ◽  
Human Blood ◽  
Human Blood Serum ◽  
Hplc Determination ◽  
Low Volume

HPLC determination of the new cardiotropic drug protecor in blood serum of animals

2006 ◽  
Vol 40 (12) ◽  
pp. 683-685
Author(s):  
S. N. Ptitsina ◽  
V. I. Bobrov ◽  
L. N. Serov ◽  
M. M. Borisov ◽  
I. Kh. Ivanova ◽  
...  
Keyword(s):  
Blood Serum ◽  
Hplc Determination
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