Influence of sulfur-amino acid content variation in plant vs animal protein on serum and tissue lipids in rats

1990 ◽  
Vol 40 (4) ◽  
pp. 297-308 ◽  
Author(s):  
Susan M. Potter ◽  
Constance V. Kies
Keyword(s):  
Amino Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Animal Protein ◽  
Sulfur Amino Acid ◽  
Content Variation ◽  
Tissue Lipids

Development of high protein soybean experimental lines with enhanced sulfur amino acid content

2019 ◽  
Author(s):  
◽  
Alaa Alaswad
Keyword(s):  
Amino Acid ◽  
Acid Content ◽  
Nutritive Value ◽  
Animal Feed ◽  
Amino Acid Content ◽  
The United States ◽  
High Protein ◽  
Sulfur Amino Acid ◽  
Soybean Line ◽  
Modest Improvement

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Soybean is one of the most important field crops grown in the United States. Commercial soybeans contain about 36 - 40% protein and 18 - 20% oil, by dry weight. Most of the soybean produced in the U.S. is utilized for oil production and animal feed. Even though soybean is a rich source of protein for livestock and humans, the nutritional quality of soybean can be further enhanced if the concentration of sulfur containing amino acids, methionine and cysteine, can be increased. Previously, our lab has employed transgenic approaches to increase the nutritive value of soybean by expressing heterologous sulfur-rich seed proteins and by manipulating the sulfur assimilatory pathway. Even though modest improvement in the nutritive value has been obtained by these approaches, till now no high yielding soybean cultivars with high protein and high sulfur amino acid content has been developed. Such cultivars will be of great value for the agricultural industry especially in animal feed. In this study, I have crossed a South Korean high protein soybean line (Lee5) with a transgenic soybean line with elevated sulfur amino acid content (CS) and developed soybean experimental lines that were advanced to F4:5 seeds. Theses experimental lines exhibit both high protein and high sulfur amino acid content. The protein content of their seeds has been increased 8 to 14% while their oil content has decreased 5 to 7% when compared with CS. Furthermore, I have successfully introduced the cytosolic isoform of O-acetylserine sulfhydrylase (OASS), the overexpressed transgene, from CS into these crosses. The activity of OASS has been increased 5 to 20-fold in experimental lines when compared to Lee5.

Nitrogen Lowers the Sulfur Amino Acid Content of Soybean (Glycine max[L.] Merr.) by Regulating the Accumulation of Bowman−Birk Protease Inhibitor

2005 ◽  
Vol 53 (16) ◽  
pp. 6347-6354 ◽  
Author(s):  
Hari B. Krishnan ◽  
John O. Bennett ◽  
Won-Seok Kim ◽  
Ammulu Hari Krishnan ◽  
Thomas P. Mawhinney
Keyword(s):  
Glycine Max ◽  
Amino Acid ◽  
Protease Inhibitor ◽  
Acid Content ◽  
Amino Acid Content ◽  
Sulfur Amino Acid ◽  
Glycine Max L

scholarly journals The Cause of Change in Egg Cycle by Varying Sulfur Amino Acid Content of the Diet

1970 ◽  
Vol 49 (5) ◽  
pp. 1359-1361 ◽  
Author(s):  
R.S. Moreno ◽  
H.R. Wilson ◽  
B.L. Damron ◽  
R.H. Harms
Keyword(s):  
Amino Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Sulfur Amino Acid

Development of soybean experimental lines with enhanced protein and sulfur amino acid content

Plant Science ◽  
2021 ◽  
pp. 110912
Author(s):  
Alaa A. Alaswad ◽  
Bo Song ◽  
Nathan W. Oehrle ◽  
William J. Wiebold ◽  
Thomas P. Mawhinney ◽  
...  
Keyword(s):  
Amino Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Sulfur Amino Acid

scholarly journals Analysis and Comparison of Human Prostasomes Amino Acid Content Variation in Normal Men and Infertile Men-A Clinical Relevance Study for Effective Diagnosis Method for Human Male Infertility

2021 ◽  
Vol 11 (2) ◽  
pp. 1537-1548
Author(s):  
Vignesh D.
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Clinical Relevance ◽  
Amino Acid Content ◽  
World Health ◽  
Infertile Men ◽  
Content Variation ◽  
Diagnosis Method ◽  
Normal Men

Aim: The main objective of this study is to compare and analyse human prostasomes amino acid content variation in normal men semen samples and infertile men semen samples for identification of clinical relevance. Materials and methods: Semen samples were collected from normal men (N=32) and from infertile men (N=32) and by following the standard world health organisation protocol semen analysis was done. Amino acid quantification was done by using amino acid analyzer. Prostasomes were separated from spermatozoa and seminal plasma by using centrifugation technique at 95000 RPM for 90 mins. Results: Independent sample T-test was carried out and shows that proline and alanine amino acids concentration (p<0.01) statistically significant compared with fertile men and infertile men. High concentration of amino acids in prostasomes were found in fertile men samples (18.09 ± 0.20 μmoles/L) when compared with infertile men samples (15.12± 0.37 μmoles/L). Conclusion: Amino acid in prostasomes plays an important role in the fertilization; the change in the concentration of amino acid in prostasomes leads to infertility of men. Here we found that the concentration of amino acids is high in fertile men when compared to infertile men which could act as an innovative diagnosis method for infertility.

Feed intake and digestion responses in sheep to the addition of inorganic sulfur to a herbage diet of low sulfur content

1988 ◽  
Vol 39 (6) ◽  
pp. 1107 ◽  
Author(s):  
RH Weston ◽  
JR Lindsay ◽  
DB Purser ◽  
GLR Gordon ◽  
P Davis
Keyword(s):  
Amino Acids ◽  
Organic Matter ◽  
Amino Acid ◽  
Feed Intake ◽  
Acid Content ◽  
Amino Acid Content ◽  
Feed Consumption ◽  
Sulfur Amino Acid ◽  
Fungal Activity ◽  
Low Sulfur

Young sheep were used in feed intake and digestion studies with a wheaten straw diet of low sulfur (S) content (0.71 g S kg-1 organic matter) fed without additional S (low S diet) or containing sodium sulfate (high S diet). With the high S diet, relative to the low S diet, (i) the levels of sulfide in rumen liquor were elevated, (ii) less time was spent in ruminating activities, (iii) rumen liquor volume tended to be lower (-l0%), (iv) indigestible markers were cleared more rapidly from the rumen (approx. +16%), (v) more organic matter (OM) and acid detergent fibre were digested in the stomach and in the alimentary tract as a whole (+8-16%), (vi) more non-ammonia nitrogen (NAN) flowed into the intestines (+13%) and this NAN had a higher content of sulfur amino acids (+ 17%), (vii) a greater quantity of NAN was digested in the intestines per unit of digestible OM intake (+ 12%), (viii) the concentrations of various amino acids in venous blood were substantially lower, and (ix) fungal activity in the rumen was higher. The number of bites performed on each rumination bolus was inversely related to fungal activity in the rumen, but the latter was not related to OM digestibility or to the sulfur amino acid content of the NAN flowing into the intestines. The OM digestibility was inversely related to the rate of clearance of markers from the rumen with the low S diet but not the high S diet. Voluntary feed consumption was similar with both diets, and in the low S diet it was inversely related to OM digestibility and positively related to marker clearance rate from the rumen. Digestible OM intake under conditions of ad libitum feeding was higher with the high S diet (+11%) and positively related to fungal activity in the rumen and to the sulfur amino acid content of the NAN flowing into the intestines. The data indicate that an inadequate amount of S in the low S diet impaired the metabolism of the rumen microbiota which in turn affected variables relating to digestion and metabolism. A quantitatively significant role for the anaerobic fungi in the structural degradation of fibre is suggested and attention is drawn to the need for studies on both feed consumption regulation with low S diets and the relation between herbage S status and the sulfur amino acid content of the rumen microbiota.

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