In vitro release of free fatty acids by adipose tissue in young and old nephrotic rats

1962 ◽  
Vol 18 (9) ◽  
pp. 418-419 ◽  
Author(s):  
H. Altschuler ◽  
M. Lieberson ◽  
J. J. Spitzer
1969 ◽  
Vol 25 (2) ◽  
pp. 128-128 ◽  
Author(s):  
A. Kershbaum ◽  
H. Osada ◽  
D. J. Pappajohn ◽  
S. Bellet

1962 ◽  
Vol 25 (2) ◽  
pp. 189-198 ◽  
Author(s):  
R. M. BUCKLE

SUMMARY The quantity of free fatty acids (FFA) released from rat epididymal fat pads in vitro and their concentration within the tissue were determined. The addition of adrenaline, adrenocorticotrophic hormone (ACTH), thyroid stimulating hormone (TSH) and growth hormone (GH) each increased the release of FFA, and their respective minimum effective concentrations were 0·125, 0·004, 0·5 and 1·25 μg./ml. of medium. In every case, the increased release of FFA was associated with a rise in the quantity present within the pads, and the amount released closely paralleled their concentration within the tissue. It is suggested that the stimulatory effect of all four hormones on the release of FFA from adipose tissue is largely a manifestation of their activity of increasing the concentration of FFA within the cells, and this they do by facilitating the net conversion of storage triglyceride to fatty acid. The significance of the relative activities of the hormones in vitro is discussed and compared with their fatty acid mobilizing effects in vivo.


1990 ◽  
Vol 68 (4) ◽  
pp. 1024-1029 ◽  
Author(s):  
T. A. Peterla ◽  
C. G. Scanes

Abstract The effects of the β-adrenergic agonists isoproterenol, cimaterol, ractopamine and clenbuterol on lipolysis (release of glycerol and free fatty acids) and lipogenesis (incorporation of 14C into fatty acids from [14C]glucose) was examined in porcine adipose tissue explants in vitro. Lipolysis was stimulated by isoproterenol, cimaterol or ractopamine but not by clenbuterol. Insulin reduced the lipolytic effects of the β-adrenergic agonists (isoproterenol, cimaterol and ractopamine). Lipogenesis was inhibited by all β-adrenergic agonists tested (isoproterenol, cimaterol, ractopamine and clenbuterol). The antilipogenic effect of the β-adrenergic agonists was reduced by the presence of insulin in the incubation. Although effects of the different β-adrenergic agonists varied, all had some direct effects that could be expected to reduce adipose accretion. Effects of β-adrenergic agonists in the pig are due in part to direct effects on adipose tissue.


1971 ◽  
Vol 70 (3) ◽  
pp. 407-415 ◽  
Author(s):  
Susumu YASUOKA ◽  
Setsuro FUJII

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