Ultrastructural localization of arylsulfatase B in mitochondria of epithelial cells of the proximal convoluted tubules of the rat kidney

1971 ◽  
Vol 27 (2) ◽  
pp. 187-189 ◽  
Author(s):  
T. Makita ◽  
E. B. Sandborn
Keyword(s):  
Epithelial Cells ◽  
Rat Kidney ◽  
Ultrastructural Localization ◽  
Arylsulfatase B ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

scholarly journals Immunohistochemical Localization of Arginine and Citrulline in Rat Renal Tissue

1997 ◽  
Vol 45 (6) ◽  
pp. 875-881 ◽  
Author(s):  
Eiko Aoki ◽  
Ikuo K. Takeuchi
Keyword(s):  
Amino Acids ◽  
Epithelial Cells ◽  
Rat Kidney ◽  
Immunohistochemical Method ◽  
Moderate Intensity ◽  
Outer Medulla ◽  
Collecting Ducts ◽  
Outer Stripe ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

Using antibodies highly specific for L-arginine and L-citrulline, we localized these amino acids in rat kidney with immunohistochemical methods. Highest levels of arginine immunoreactivity were observed in epithelial cells of proximal tubules in the outer stripe of the outer medulla and the collecting ducts in the cortex. Staining intensity of proximal convoluted tubules in the outer stripe decreased from the inner side to the outer side. In the inner medulla, collecting ducts were labeled with moderate intensity. Staining within the cortex was apparent only with collecting ducts. Citrulline immunoreactivity was localized in the epithelial cells of collecting ducts both in the cortex and medulla. Immunoreactivity was also found in glomerular podocytes and in the epithelial cells of proximal convoluted tubules in the outer medulla. These localizations were different from those of other amino acids previously reported. The precise cellular distribution of arginine and citrulline in rat kidney was determined for the first time by an immunohistochemical method in the present study.

scholarly journals HISTOCHEMICAL STUDIES ON THE UPTAKE OF HORSERADISH PEROXIDASE BY RAT KIDNEY SLICES

1965 ◽  
Vol 27 (2) ◽  
pp. 305-312 ◽  
Author(s):  
A. T. Miller ◽  
D. M. Hale ◽  
K. D. Alexander
Keyword(s):  
Horseradish Peroxidase ◽  
Intracellular Transport ◽  
Rat Kidney ◽  
Kidney Slices ◽  
Metabolic Conditions ◽  
Peroxidase Uptake ◽  
Free Media ◽  
Energy Dependent ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

When rat kidney slices were incubated in the presence of horseradish peroxidase, there was an energy-dependent uptake of the protein by the cells of the kidney tubules. The uptake was greatest in the proximal convoluted tubules and in the thick ascending limbs of the loops of Henle; it was abolished by cold, anoxia, 2,4-dinitrophenol, and fluoroacetate, and was more readily depressed by unfavorable metabolic conditions in the proximal convoluted tubules than in the thick ascending limbs. Protein uptake was inhibited when the kidney slices were incubated in electrolyte-free media. In sodium chloride solutions, uptake was reduced as sodium was progressively replaced by choline, and ouabain inhibited uptake in the proximal convoluted tubules, but not in the thick ascending limbs. To a limited extent, lithium could replace sodium in the incubation medium with no depression of peroxidase uptake. These results suggest that a sodium-stimulated, ouabain-sensitive ATPase may be involved in the uptake of protein by cells of the kidney tubule. The intracellular transport of peroxidase in cells of the proximal convoluted tubules was abolished by cold, anoxia, and 2,4-dinitrophenol, but it was not affected by concentrations of ouabain which inhibited the uptake of the protein.

Source and Handling of Renal Dopamine: Its Physiological Importance

Physiology ◽  
1994 ◽  
Vol 9 (3) ◽  
pp. 128-134 ◽  
Author(s):  
P Soares-da-Silva
Keyword(s):  
Epithelial Cells ◽  
Monoamine Oxidase ◽  
Dopaminergic System ◽  
Physiological Importance ◽  
Renal Dopamine ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

The renal dopaminergic system is based in the epithelial cells of proximal convoluted tubules, which use circulating or filtered L-dopa for fast decarboxylation into dopamine. Monoamine oxidase is the main enzyme involved in its metabolism.

scholarly journals THE RELATIVE REACTION WITHIN LIVING MAMMALIAN TISSUES

1928 ◽  
Vol 47 (5) ◽  
pp. 797-820 ◽  
Author(s):  
Philip D. McMaster ◽  
Robert Elman
Keyword(s):  
Epithelial Cells ◽  
Transitional Zone ◽  
Color Pattern ◽  
Complete Suppression ◽  
Loop Of Henle ◽  
Acid Urine ◽  
Mammalian Tissues ◽  
Convoluted Tubules ◽  
Bright Blue ◽  
Proximal Convoluted Tubules

The tubules of the mammalian kidney, vitally stained with erythrolitmin, show a significant color pattern, the cells of certain regions appearing bright blue and others brilliant red. The dye is segregated within the cytoplasm, staining fine granules diffusely. Under normal circumstances of renal function erythrolitmin is stored in the lining cells of the glomerulus and the epithelial cells of the proximal convoluted tubules in the blue, alkaline form. In the cells of the final portion of the distal convoluted tubules the dye is red. Between these regions a narrow transitional zone can be found, at times above, at times below the loop of Henle, in which the erythrolitmin-stained granules in the tubule cells are violet, or red and blue within the same cell. Alterations in the relative reaction of certain regions of the tubules, as disclosed by the color of the dye within the cells, can be induced by means that alter the reaction of the urine. When acid urine is being secreted there is a change from relative alkalinity to acidity high up in the tubular canal, in the proximal convoluted tubules. During the secretion of markedly alkaline urine the intracellular granules appear blue most of the way down the tubular canal, even to the first portion of the distal convoluted tubules. When the urine is neutral the cells above the descending limb of the loop of Henle are alkaline to erythrolitmin, and those below this point appear acid. The granules within the endothelial cells of the glomerulus and the epithelial cells of the first portion of the proximal convoluted tubules are always alkaline to erythrolitmin; while those within the cells of the final portion of the distal convoluted tubules are always acid regardless of the reaction of the urine. Only after complete suppression of urine as result of massive doses of acid do granules of the sort first mentioned manifest the color indicative of acidity. The interpretation of the findings waits upon further work.

scholarly journals Cellular and subcellular localization of enzymes of arginine metabolism in rat kidney

1992 ◽  
Vol 282 (2) ◽  
pp. 369-375 ◽  
Author(s):  
S N Dhanakoti ◽  
M E Brosnan ◽  
G R Herzberg ◽  
J T Brosnan
Keyword(s):  
Rat Kidney ◽  
Subcellular Fractionation ◽  
Kidney Cortex ◽  
Ornithine Aminotransferase ◽  
Outer Medulla ◽  
Argininosuccinate Synthase ◽  
Distal Tubules ◽  
Convoluted Tubules ◽  
Inner Cortex ◽  
Proximal Convoluted Tubules

Rat kidneys extract citrulline derived from the intestinal metabolism of glutamine and convert it stoichiometrically into arginine. This pathway constitutes the major endogenous source of arginine. We investigated the localization of enzymes of arginine synthesis, argininosuccinate synthase and lyase, and of breakdown, arginase and ornithine aminotransferase, in five regions of rat kidney, in cortical tubule fractions and in subcellular fractions of cortex. Argininosuccinate synthase and lyase were found almost exclusively in cortex. Arginase and ornithine aminotransferase were found in inner cortex and outer medulla. Since cortical tissue primarily consists of proximal convoluted and straight tubules, distal tubules and glomeruli, we prepared cortical tubule fragments by collagenase digestion of cortices and fractionated them on a Percoll gradient. Argininosuccinate synthase and lyase were found to be markedly enriched in proximal convoluted tubules, whereas less than 10% of arginase and ornithine aminotransferase, were recovered in this fraction. Arginine production from citrulline was also enriched in proximal convoluted tubules. Subcellular fractionation of kidney cortex revealed that argininosuccinate synthase and lyase are cytosolic. We therefore conclude that arginine synthesis occurs in the cytoplasm of the cells of the proximal convoluted tubule.

Effect of benzolamide on luminal pH in proximal convoluted tubules of the rat kidney

1978 ◽  
Vol 375 (1) ◽  
pp. 39-43 ◽  
Author(s):  
F. Lang ◽  
P. Quehenberger ◽  
R. Greger ◽  
H. Oberleithner
Keyword(s):  
Rat Kidney ◽  
Luminal Ph ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

scholarly journals APOBEC3G Expression Is Restricted to Epithelial Cells of the Proximal Convoluted Tubules and Is Not Expressed in the Glomeruli of Macaques

2006 ◽  
Vol 55 (1) ◽  
pp. 63-70 ◽  
Author(s):  
M. Sarah Hill ◽  
Autumn Ruiz ◽  
Lisa M. Gomez ◽  
Jean-Marie Miller ◽  
Nancy E.J. Berman ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Convoluted Tubules ◽  
Apobec3g Expression ◽  
Proximal Convoluted Tubules

Immunoelectron microscopic localization of α2u-globulin in the male rat kidney after gasoline administration

1987 ◽  
Vol 45 ◽  
pp. 872-873
Author(s):  
B.D. Garg ◽  
M.J. Olson ◽  
L. Li ◽  
M.A. Mancini ◽  
A.K. Roy
Keyword(s):  
Epithelial Cells ◽  
Rat Kidney ◽  
Male Rats ◽  
Male Rat ◽  
Ultrastructural Examination ◽  
Unleaded Gasoline ◽  
Once Daily ◽  
Gasoline Hydrocarbons ◽  
Secondary Lysosomes ◽  
Convoluted Tubules

Exposure of male rats to unleaded gasoline and other volatile hydrocarbons causes acute nephrotoxicity with accumulation of hyalin droplets (HD) in epithelial cells of the proximal convoluted tubules (PCT). We have shown that HD accumulated after gasoline intoxication are partially composed of α2u-globulin, a protein found exclusively in adult male rats. Ultrastructural examination revealed HD to be enlarged phagolysosomes (secondary lysosomes). Metabolites of gasoline hydrocarbons are hypothesized to bind reversibly to α2u-globulin, perhaps limiting lysosomal degradation of this protein. α2u-Globulin accumulation may also result from hydrocarbon-mediated inhibition of lysosomal proteolytic enzyme activity. These studies were conducted to determine the distribution of α2u-globulin within epithelial cells of PCT during gasoline intoxication.Male F-344 rats were administered unleaded gasoline or 0.9% NaCl solution (2.0 mL/Kg, p.o., once daily, nine days) and 2 mm slices of saline-perfused kidney were fixed by immersion in 2.5% glutaraldehyde, 0.1 M sodium cacodylate (pH 7.4).

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