Effect of benzolamide on luminal pH in proximal convoluted tubules of the rat kidney

1978 ◽  
Vol 375 (1) ◽  
pp. 39-43 ◽  
Author(s):  
F. Lang ◽  
P. Quehenberger ◽  
R. Greger ◽  
H. Oberleithner
Keyword(s):  
Rat Kidney ◽  
Luminal Ph ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

scholarly journals HISTOCHEMICAL STUDIES ON THE UPTAKE OF HORSERADISH PEROXIDASE BY RAT KIDNEY SLICES

1965 ◽  
Vol 27 (2) ◽  
pp. 305-312 ◽  
Author(s):  
A. T. Miller ◽  
D. M. Hale ◽  
K. D. Alexander
Keyword(s):  
Horseradish Peroxidase ◽  
Intracellular Transport ◽  
Rat Kidney ◽  
Kidney Slices ◽  
Metabolic Conditions ◽  
Peroxidase Uptake ◽  
Free Media ◽  
Energy Dependent ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

When rat kidney slices were incubated in the presence of horseradish peroxidase, there was an energy-dependent uptake of the protein by the cells of the kidney tubules. The uptake was greatest in the proximal convoluted tubules and in the thick ascending limbs of the loops of Henle; it was abolished by cold, anoxia, 2,4-dinitrophenol, and fluoroacetate, and was more readily depressed by unfavorable metabolic conditions in the proximal convoluted tubules than in the thick ascending limbs. Protein uptake was inhibited when the kidney slices were incubated in electrolyte-free media. In sodium chloride solutions, uptake was reduced as sodium was progressively replaced by choline, and ouabain inhibited uptake in the proximal convoluted tubules, but not in the thick ascending limbs. To a limited extent, lithium could replace sodium in the incubation medium with no depression of peroxidase uptake. These results suggest that a sodium-stimulated, ouabain-sensitive ATPase may be involved in the uptake of protein by cells of the kidney tubule. The intracellular transport of peroxidase in cells of the proximal convoluted tubules was abolished by cold, anoxia, and 2,4-dinitrophenol, but it was not affected by concentrations of ouabain which inhibited the uptake of the protein.

scholarly journals Cellular and subcellular localization of enzymes of arginine metabolism in rat kidney

1992 ◽  
Vol 282 (2) ◽  
pp. 369-375 ◽  
Author(s):  
S N Dhanakoti ◽  
M E Brosnan ◽  
G R Herzberg ◽  
J T Brosnan
Keyword(s):  
Rat Kidney ◽  
Subcellular Fractionation ◽  
Kidney Cortex ◽  
Ornithine Aminotransferase ◽  
Outer Medulla ◽  
Argininosuccinate Synthase ◽  
Distal Tubules ◽  
Convoluted Tubules ◽  
Inner Cortex ◽  
Proximal Convoluted Tubules

Rat kidneys extract citrulline derived from the intestinal metabolism of glutamine and convert it stoichiometrically into arginine. This pathway constitutes the major endogenous source of arginine. We investigated the localization of enzymes of arginine synthesis, argininosuccinate synthase and lyase, and of breakdown, arginase and ornithine aminotransferase, in five regions of rat kidney, in cortical tubule fractions and in subcellular fractions of cortex. Argininosuccinate synthase and lyase were found almost exclusively in cortex. Arginase and ornithine aminotransferase were found in inner cortex and outer medulla. Since cortical tissue primarily consists of proximal convoluted and straight tubules, distal tubules and glomeruli, we prepared cortical tubule fragments by collagenase digestion of cortices and fractionated them on a Percoll gradient. Argininosuccinate synthase and lyase were found to be markedly enriched in proximal convoluted tubules, whereas less than 10% of arginase and ornithine aminotransferase, were recovered in this fraction. Arginine production from citrulline was also enriched in proximal convoluted tubules. Subcellular fractionation of kidney cortex revealed that argininosuccinate synthase and lyase are cytosolic. We therefore conclude that arginine synthesis occurs in the cytoplasm of the cells of the proximal convoluted tubule.

scholarly journals Immunohistochemical Localization of Arginine and Citrulline in Rat Renal Tissue

1997 ◽  
Vol 45 (6) ◽  
pp. 875-881 ◽  
Author(s):  
Eiko Aoki ◽  
Ikuo K. Takeuchi
Keyword(s):  
Amino Acids ◽  
Epithelial Cells ◽  
Rat Kidney ◽  
Immunohistochemical Method ◽  
Moderate Intensity ◽  
Outer Medulla ◽  
Collecting Ducts ◽  
Outer Stripe ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

Using antibodies highly specific for L-arginine and L-citrulline, we localized these amino acids in rat kidney with immunohistochemical methods. Highest levels of arginine immunoreactivity were observed in epithelial cells of proximal tubules in the outer stripe of the outer medulla and the collecting ducts in the cortex. Staining intensity of proximal convoluted tubules in the outer stripe decreased from the inner side to the outer side. In the inner medulla, collecting ducts were labeled with moderate intensity. Staining within the cortex was apparent only with collecting ducts. Citrulline immunoreactivity was localized in the epithelial cells of collecting ducts both in the cortex and medulla. Immunoreactivity was also found in glomerular podocytes and in the epithelial cells of proximal convoluted tubules in the outer medulla. These localizations were different from those of other amino acids previously reported. The precise cellular distribution of arginine and citrulline in rat kidney was determined for the first time by an immunohistochemical method in the present study.

scholarly journals Proximal convoluted tubules of the rats kidney--a stereological analysis

2003 ◽  
Vol 3 (1) ◽  
pp. 36-39 ◽  
Author(s):  
Selma Aličelebić
Keyword(s):  
Rat Kidney ◽  
Volume Density ◽  
Kidney Cortex ◽  
Rat Kidney Cortex ◽  
Mean Values ◽  
Quantitative Parameters ◽  
Male Wistar Rats ◽  
Normal Rat ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

Background and Purpose: The aim of this work was to order quantitative parameters of the proximal convoluted tubules (PCT) in the normal rat kidney cortex. Volume density (VV), both surface and specific surface density toward interstitium (SVi and SVi/VV) and toward lumen (SVl and SVl/VV) and thickness (T) of tubules epithelium have been stereologically ordered.Material and Methods: Stereologically were analysed 170 test fields by lattice L36 on the paraffin sections of the three adult male Wistar rats kidney dyeing by PAS-method.Results: The mean values of the variables analysed were Vv=76.4% ±0.012; Svi=0.056pm-1 ±0.004; Svl=0.028pm-1 ±0.003; Svi/Vv=0.073pm-1 ±0.003; Svl/Vv=0.037pm-1 ±0.005; T=18,26pm ±0.897.Conclusions: Stereological methods are making a very valuable contribution to science over recent years. We have used unbiased stereological counting methods to obtain objective quantitative parameters of the PCT epithelium in the normal rats’ kidney cortex.

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