The cytochemical demonstration of dehydrogenases and oxidases in the cells of fungi

1968 ◽  
Vol 24 (8) ◽  
pp. 854-856 ◽  
Author(s):  
J. Reiss
Keyword(s):  
Cytochemical Demonstration

scholarly journals ENZYMATIC DIGESTION IN THE CYTOCHEMICAL DEMONSTRATION OF GLYCOGEN

1967 ◽  
Vol 15 (1) ◽  
pp. 14-20 ◽  
Author(s):  
FORTUNATO ROSA ◽  
FRANK B. JOHNSON
Keyword(s):  
Gastric Mucosa ◽  
Epoxy Resin ◽  
Liver Tissue ◽  
Osmium Tetroxide ◽  
Periodic Acid ◽  
Enzymatic Digestion ◽  
Selective Removal ◽  
Cytochemical Demonstration ◽  
Ultrathin Sections

Mounted ultrathin sections of rat fetal gastric mucosa and liver tissue, fixed in osmium tetroxide or glutaraldehyde and embedded in epoxy resin, are suitable for the cytochemical demonstration of glycogen by staining either with lead or by the periodic acid-thiosemicarbazide procedure. Enzymatic digestion with saliva or α-amylase can be performed on mounted sections of similarly treated tissues for the selective removal of glycogen prior to staining.

scholarly journals Cytochemical Demonstration of Oxidative Damage in Alzheimer Disease by Immunochemical Enhancement of the Carbonyl Reaction with 2,4-Dinitrophenylhydrazine

1998 ◽  
Vol 46 (6) ◽  
pp. 731-735 ◽  
Author(s):  
Mark A. Smith ◽  
Lawrence M. Sayre ◽  
Vernon E. Anderson ◽  
Peggy L.R. Harris ◽  
M. Flint Beal ◽  
...  
Keyword(s):  
Alzheimer Disease ◽  
Oxidative Damage ◽  
Peroxidation Of Lipids ◽  
Bulk Analysis ◽  
Age Related ◽  
Cytochemical Demonstration ◽  
Highly Sensitive ◽  
Metal Catalyzed ◽  
Specific Oxidation ◽  
Cytochemical Technique

Formation of carbonyls derived from lipids, proteins, carbohydrates, and nucleic acids is common during oxidative stress. For example, metal-catalyzed, “site-specific” oxidation of several amino acid side-chains produces aldehydes or ketones, and peroxidation of lipids generates reactive aldehydes such as malondialdehyde and hydroxynonenal. Here, using in situ 2,4-dinitrophenylhydrazine labeling linked to an antibody system, we describe a highly sensitive and specific cytochemical technique to specifically localize biomacromolecule-bound carbonyl reactivity. When this technique was applied to tissues from cases of Alzheimer disease, in which oxidative events including lipoperoxidative, glycoxidative, and other oxidative protein modifications have been reported, we detected free carbonyls not only in the disease-related intraneuronal lesions but also in other neurons. In marked contrast, free carbonyls were not found in neurons or glia in age-matched control cases. Importantly, this assay was highly specific for detecting disease-related oxidative damage because the site of oxidative damage can be assessed in the midst of concurrent age-related increases in free carbonyls in vascular basement membrane that would contaminate biochemical samples subjected to bulk analysis. These findings demonstrate that oxidative imbalance and stress are key elements in the pathogenesis of Alzheimer disease.

scholarly journals On the Cytochemical Demonstration of Aldehydes

1950 ◽  
Vol s3-91 (14) ◽  
pp. 215-216
Author(s):  
J. F. DANIELLI
Keyword(s):  
Cytochemical Demonstration

Techniques are discussed for the cytochemical demonstration of (a) free aldehydes, (b) aldehyde acetals, and (c) aldehydes formed by oxidation.

Cytochemical demonstration of the effects of the acylureas flufenoxuron and diflubenzuron on the incorporation of chitin into insect cuticle

1990 ◽  
Vol 28 (4) ◽  
pp. 367-375 ◽  
Author(s):  
Susan A. Lee ◽  
Barry S. Clarke ◽  
Donald W. Jenner ◽  
Francis A. Williamson
Keyword(s):  
Insect Cuticle ◽  
Cytochemical Demonstration
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