Histochemical and cytochemical demonstration of zinc cysteinate in the tapetum lucidum of the cat

T. Kohler

doi:10.1007/bf00493210

The cytochemical demonstration of dehydrogenases and oxidases in the cells of fungi

Cellular and Molecular Life Sciences ◽

10.1007/bf02144917 ◽

1968 ◽

Vol 24 (8) ◽

pp. 854-856 ◽

Cited By ~ 6

Author(s):

J. Reiss

Keyword(s):

Cytochemical Demonstration

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ENZYMATIC DIGESTION IN THE CYTOCHEMICAL DEMONSTRATION OF GLYCOGEN

Journal of Histochemistry & Cytochemistry ◽

10.1177/15.1.14 ◽

1967 ◽

Vol 15 (1) ◽

pp. 14-20 ◽

Cited By ~ 22

Author(s):

FORTUNATO ROSA ◽

FRANK B. JOHNSON

Keyword(s):

Gastric Mucosa ◽

Epoxy Resin ◽

Liver Tissue ◽

Osmium Tetroxide ◽

Periodic Acid ◽

Enzymatic Digestion ◽

Selective Removal ◽

Cytochemical Demonstration ◽

Ultrathin Sections

Mounted ultrathin sections of rat fetal gastric mucosa and liver tissue, fixed in osmium tetroxide or glutaraldehyde and embedded in epoxy resin, are suitable for the cytochemical demonstration of glycogen by staining either with lead or by the periodic acid-thiosemicarbazide procedure. Enzymatic digestion with saliva or α-amylase can be performed on mounted sections of similarly treated tissues for the selective removal of glycogen prior to staining.

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Cytochemical demonstration of NADH-ferricyanide reductase and nucleoside diphosphatase activities in dictyosome-like structures of guinea pig spermatocytes

Cell and Tissue Research ◽

10.1007/bf00233723 ◽

1980 ◽

Vol 211 (1) ◽

Cited By ~ 5

Author(s):

D.J. Morr� ◽

HiltonH. Mollenhauer ◽

C.M. Eppler

Keyword(s):

Guinea Pig ◽

Ferricyanide Reductase ◽

Cytochemical Demonstration ◽

Nucleoside Diphosphatase

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Cytochemical Demonstration of Oxidative Damage in Alzheimer Disease by Immunochemical Enhancement of the Carbonyl Reaction with 2,4-Dinitrophenylhydrazine

Journal of Histochemistry & Cytochemistry ◽

10.1177/002215549804600605 ◽

1998 ◽

Vol 46 (6) ◽

pp. 731-735 ◽

Cited By ~ 158

Author(s):

Mark A. Smith ◽

Lawrence M. Sayre ◽

Vernon E. Anderson ◽

Peggy L.R. Harris ◽

M. Flint Beal ◽

...

Keyword(s):

Alzheimer Disease ◽

Oxidative Damage ◽

Peroxidation Of Lipids ◽

Bulk Analysis ◽

Age Related ◽

Cytochemical Demonstration ◽

Highly Sensitive ◽

Metal Catalyzed ◽

Specific Oxidation ◽

Cytochemical Technique

Formation of carbonyls derived from lipids, proteins, carbohydrates, and nucleic acids is common during oxidative stress. For example, metal-catalyzed, “site-specific” oxidation of several amino acid side-chains produces aldehydes or ketones, and peroxidation of lipids generates reactive aldehydes such as malondialdehyde and hydroxynonenal. Here, using in situ 2,4-dinitrophenylhydrazine labeling linked to an antibody system, we describe a highly sensitive and specific cytochemical technique to specifically localize biomacromolecule-bound carbonyl reactivity. When this technique was applied to tissues from cases of Alzheimer disease, in which oxidative events including lipoperoxidative, glycoxidative, and other oxidative protein modifications have been reported, we detected free carbonyls not only in the disease-related intraneuronal lesions but also in other neurons. In marked contrast, free carbonyls were not found in neurons or glia in age-matched control cases. Importantly, this assay was highly specific for detecting disease-related oxidative damage because the site of oxidative damage can be assessed in the midst of concurrent age-related increases in free carbonyls in vascular basement membrane that would contaminate biochemical samples subjected to bulk analysis. These findings demonstrate that oxidative imbalance and stress are key elements in the pathogenesis of Alzheimer disease.

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