The influence ofBordetella pertussis on the kinetics of antibody production to sheep red blood cells in NMRI mice

1968 ◽  
Vol 24 (8) ◽  
pp. 832-833
Author(s):  
H. Finger ◽  
P. Emmerling
Keyword(s):  
Red Blood Cells ◽  
Antibody Production ◽  
Blood Cells ◽  
Nmri Mice ◽  
Sheep Red Blood Cells ◽  
Ofbordetella Pertussis ◽  
Kinetics Of

Kinetics of Na-Li exchange in high and low K sheep red blood cells

1989 ◽  
Vol 257 (1) ◽  
pp. C58-C64 ◽  
Author(s):  
K. H. Ryu ◽  
N. C. Adragna ◽  
P. K. Lauf
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Cell Types ◽  
Sheep Red Blood Cells ◽  
High K ◽  
Ping Pong ◽  
Order Of Magnitude ◽  
Low K ◽  
Kinetics Of ◽  
System Operating

The kinetic parameters and transport mechanism of Na-Li exchange were studied in both low K (LK) and high K (HK) sheep red blood cells with cellular Na [( Na]i) and Li concentrations [( Li]i) adjusted by the nystatin technique (Nature New Biol. 244: 47-49, 1973 and J. Physiol. Lond. 283: 177-196, 1978). Maximum velocities (Vm) for Li fluxes and half-activation constants (K1/2) for Li and Na of the Na-Li exchanger were determined. The K1/2 values for both Li and Na appeared to be similar in both cell types, although they were about two to three times lower on the inside than on the outside of the membrane. Furthermore, the K1/2 values for Li were at least an order of magnitude smaller than those for Na, suggesting substantial affinity differences for these two cations. The Vm values for Li fluxes, on the other hand, appear to be lower in HK than in LK cells. When Na and Li fluxes were measured simultaneously, a trans stimulatory effect by Na on Li fluxes was observed. From measurements of Li influx at different concentrations of external Li and different [Na]i, the ratio of the apparent Vm to the apparent external Li affinity was calculated to be independent of [Na]i for both types of sheep red blood cells. Similar trans effects of external Na were observed on Li efflux at varying [Li]i. These results are expected for a system operating by a “ping-pong” mechanism.

Differentiation of Na(+)-K+ pumps of low-K+ sheep red blood cells is promoted by Lp membrane antigens

1993 ◽  
Vol 265 (1) ◽  
pp. C99-C105 ◽  
Author(s):  
Z. C. Xu ◽  
P. B. Dunham ◽  
B. Dyer ◽  
R. Blostein
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Rat Kidney ◽  
Striking Increase ◽  
Sheep Red Blood Cells ◽  
High K ◽  
Membrane Antigens ◽  
Low K ◽  
Kinetics Of

Na(+)-K+ pumps of red blood cells from sheep of the low-K+ (LK) phenotype undergo differentiation during circulation, manifested in part by a striking increase in sensitivity to inhibition by intracellular K+ (Ki). Pumps of red blood cells from sheep from the allelic phenotype, high K+ (HK), do not undergo this type of maturation. The hypothesis was tested that the Lp antigen, found on LK but not HK cells, is responsible for the maturation of LK pumps. Lp antigens have been shown to inhibit LK pumps because anti-Lp antibody stimulates the pumps by relieving inhibition by the antigen. Lp antigens were recently shown to be molecular entities separate from Na(+)-K+ pumps [Xu, Z.-C., P. Dunham, J. Munzer, J. Silvius, and R. Blostein. Am. J. Physiol. 263 (Cell Physiol. 32): C1007-C1014, 1992]. The test of the hypothesis was to modify the Lp antigens of immature LK red blood cells with two kinds of treatments, anti-Lp antibody and trypsinization (which cleaves Lp), and to observe the effects of these treatments on maturation of pumps during culture of the cells in vitro. Both of these treatments prevented the maturation of the kinetics of the pumps to the Ki-sensitive pattern, supporting the hypothesis that interaction of the pumps with Lp antigens is responsible for the maturation of the pumps. Strong supportive evidence came from experiments on Na(+)-K+ pumps from rat kidney delivered into immature LK sheep red blood cells by microsome fusion.(ABSTRACT TRUNCATED AT 250 WORDS)

Kinetics of primary antibody responses to sheep red blood cells in birds: a literature review and new data from great tits and European starlings

2007 ◽  
Vol 57 (1) ◽  
pp. 79-95 ◽  
Author(s):  
Tinne Snoeijs ◽  
Evi Van Den Steen ◽  
Marcel Eens ◽  
Rianne Pinxten
Keyword(s):  
Immune Response ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Bird Species ◽  
Great Tit ◽  
Primary Antibody ◽  
Plateau Phase ◽  
Sheep Red Blood Cells ◽  
Antibody Levels ◽  
Kinetics Of

AbstractMeasuring the primary antibody immune response to an injection with sheep red blood cells (SRBC) is a routine application in poultry science. Recently, this technique is also becoming an increasingly popular tool to investigate humoral immunocompetence in free-living birds in studies of ecological immunology and immunotoxicology. However, an extensive search of the literature revealed that many of these studies have been measuring the primary immune response to SRBC without verifying first when maximum levels of antibodies are reached in the bird species under study. In addition, most studies assessed antibody titres to SRBC approximately 6 days after their inoculation assuming a similar pattern as found in poultry. We tested this assumption of a uniform pattern of kinetics of the primary humoral immune response to SRBC across bird species and investigated it in detail in two important model species, namely the great tit Parus major and the European starling Sturnus vulgaris. In general, the pattern was found to be the same in both passerine species and strongly resembled the pattern observed in chickens. Maximum antibody levels (the plateau phase) were reached on, respectively, day 5 and day 6 (with day of inoculation = day 0) of the immune response in the great tit and the starling and lasted for 3 days in both species. We found no effects of age, sex or time spent in captivity. Furthermore, our findings suggest that antibody levels to SRBC should not be obligatory assessed during the plateau phase of maximum antibody levels although it still remains most accurate to do so or at least shortly before or after it. Overall, we conclude that antibody levels have been reliably assessed in most avian studies using this technique.

scholarly journals PRIMARY STIMULATION AND MEASUREMENT OF ANTIBODY PRODUCTION TO SHEEP RED BLOOD CELLS IN VITRO

1967 ◽  
Vol 126 (2) ◽  
pp. 347-356 ◽  
Author(s):  
W. A. Robinson ◽  
J. Marbrook ◽  
E. Diener
Keyword(s):  
Red Blood Cells ◽  
Antibody Production ◽  
Blood Cells ◽  
Actinomycin D ◽  
Plaque Formation ◽  
Spleen Cells ◽  
Sheep Erythrocytes ◽  
Sheep Red Blood Cells ◽  
Number Of Cells

A method for the primary stimulation and measurement of antibody production to sheep red blood cells in vitro has been described. In this system when mouse spleen cells are incubated with sheep erythrocytes large complement-dependent plaques are formed. The number and size of plaques increases from day 1 to day 3 of incubation with an average of 4.4 plaque areas per 1 x 106 cells plated at day 3. There is a linear relationship between the number of spleen cells plated and the number of plaques formed. Plaque formation is inhibited by colchicine, actinomycin D, and rabbit anti-mouse globulin. This system offers a possible means for the direct in vitro measurement of the number of cells in a population susceptible to antigenic stimulation by sheep erythrocytes.

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