Ultrastructural changes of the trabecular meshwork of the monkey (Macaca speciosa) following irradiation with argon laser light

1984 ◽  
Vol 221 (6) ◽  
pp. 249-261 ◽  
Author(s):  
Eugen van der Zypen ◽  
Franz Fankhauser
Author(s):  
Burton B. Silver ◽  
Theodore Lawwill

Dutch-belted 1 to 2.5 kg anesthetized rabbits were exposed to either xenon or argon laser light administered in a broad band, designed to cover large areas of the retina. For laser exposure, the pupil was dilated with atropine sulfate 1% and pheny lephrine 10%. All of the laser generated power was within a band centered at 5145.0 Anstroms. Established threshold for 4 hour exposures to laser irradiation are in the order of 25-35 microwatts/cm2. Animals examined for ultrastructural changes received 4 hour threshold doses. These animals exhibited ERG, opthalmascopic, and histological changes consistent with threshold damage.One month following exposure the rabbits were killed with pentobarbitol. The eyes were immediately enucleated and dissected while bathed in 3% phosphate buffered gluteraldehyde.


2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Jeffrey R. SooHoo ◽  
Leonard K. Seibold ◽  
David A. Ammar ◽  
Malik Y. Kahook

Purpose. To compare morphologic changes in human trabecular meshwork (TM) after selective laser trabeculoplasty (SLT) and argon laser trabeculoplasty (ALT).Design. Laboratory evaluation of ex vivo human eye TM after laser trabeculoplasty.Methods. Corneoscleral rims from human cadaver eyes were sectioned and treated with varying powers of either SLT or ALT. Specimens were examined using light microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM).Results. TEM of SLT at all powers resulted in disrupted TM cells with cracked and extracellular pigment granules. SEM of SLT samples treated at high power revealed tissue destruction with scrolling of trabecular beams. SEM of ALT-treated tissue showed increasing destruction with exposure to higher power. The presence or absence of “champagne” bubbles during SLT did not alter the histologic findings.Conclusions. SLT-treated human TM revealed disruption of TM cells with cracked, extracellular pigment granules, particularly at higher treatment powers. Tissue scrolling was noted at very high SLT energy levels. ALT-treated tissue showed significant damage to both the superficial and deeper TM tissues in a dose-dependent fashion. Further studies are needed to guide titration of treatment power to maximize the IOP-lowering effect while minimizing both energy delivered and damage to target tissues.


1985 ◽  
Vol 5 (3) ◽  
pp. 249-251
Author(s):  
Kenichi Ido ◽  
Hitoshi Takahashi ◽  
Tomohiko Terada ◽  
Hiroyoshi Arai ◽  
Tetsuo Kasano ◽  
...  

1998 ◽  
Vol 7 (1) ◽  
pp. 45???49 ◽  
Author(s):  
Rong-Fang Wang ◽  
Robert A. Schumer ◽  
Janet B. Serle ◽  
Steven M. Podos

1993 ◽  
Vol 38 (1) ◽  
pp. 13391J ◽  
Author(s):  
Ed Shipp ◽  
Renee Roelofs ◽  
Enrico Togneri ◽  
Raymond Wright ◽  
David Atkinson ◽  
...  

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