The effect of short term exposures to 100% oxygen on the fine structure of proximal convoluted tubules

1970 ◽  
Vol 26 (10) ◽  
pp. 1124-1125 ◽  
Author(s):  
R. T. Hess ◽  
D. B. Menzel
Keyword(s):  
Fine Structure ◽  
Short Term ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

Patch clamp study on primary culture of isolated proximal convoluted tubules

1988 ◽  
Vol 413 (1) ◽  
pp. 51-61 ◽  
Author(s):  
J. Merot ◽  
M. Bidet ◽  
B. Gachot ◽  
S. Le Maout ◽  
M. Tauc ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Primary Culture ◽  
Clamp Study ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

Organic solutes in fluid absorption by renal proximal convoluted tubules

1976 ◽  
Vol 231 (2) ◽  
pp. 627-637 ◽  
Author(s):  
M Burg ◽  
C Patlak ◽  
N Green ◽  
D Villey
Keyword(s):  
Epithelial Cell ◽  
Organic Solutes ◽  
Fluid Absorption ◽  
Transepithelial Voltage ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

Proximal convoluted tubules were dissected from rabbit kidneys and perfused with artificial solutions in vitro. The effect of various organic solutes on rate of fluid absorption and transepithelial voltage was tested by removing solutes from or adding them to perfusate and/or bath. Omission of albumin from the bath caused rate of fluid absorption to descrease 33% without any change in voltage. Omission of glucose, lactate, alanine, and citrate from the bath had no effect. In contrast, when they were removed from perfusate, rate of fluid absorption fell by 45-75% (depending on whether they were replaced by NaCl or mannitol and NaCl), and voltage (normally negative in lymen) decreased to near zero. Adding glucose or alanine individually to perfusate caused a small increase in rate of fluid absorption and a relatively large increase in voltage. alpha-Methyl-D-glucoside and cycloleucine (which are transported but not metabolized) had effects similar to glucose and alanine, except that voltage changes were not as great. Phlorizin (10(-5) M in perfusate) had the same effect as removing glucose from perfusate. When glucose and alanine were added to perfusate, epithelial cell swelled significantly. Lactate and citrate also caused rate of fluid absorption to increase when they were added to perfusate, but they did not affect transepithelial voltage nor did they cause cells to swell significantly. Possible mechanisms of these effects and the role of organic solutes in fluid absorption by proximal convoluted tubules are discussed.

scholarly journals HISTOCHEMICAL STUDIES ON THE UPTAKE OF HORSERADISH PEROXIDASE BY RAT KIDNEY SLICES

1965 ◽  
Vol 27 (2) ◽  
pp. 305-312 ◽  
Author(s):  
A. T. Miller ◽  
D. M. Hale ◽  
K. D. Alexander
Keyword(s):  
Horseradish Peroxidase ◽  
Intracellular Transport ◽  
Rat Kidney ◽  
Kidney Slices ◽  
Metabolic Conditions ◽  
Peroxidase Uptake ◽  
Free Media ◽  
Energy Dependent ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

When rat kidney slices were incubated in the presence of horseradish peroxidase, there was an energy-dependent uptake of the protein by the cells of the kidney tubules. The uptake was greatest in the proximal convoluted tubules and in the thick ascending limbs of the loops of Henle; it was abolished by cold, anoxia, 2,4-dinitrophenol, and fluoroacetate, and was more readily depressed by unfavorable metabolic conditions in the proximal convoluted tubules than in the thick ascending limbs. Protein uptake was inhibited when the kidney slices were incubated in electrolyte-free media. In sodium chloride solutions, uptake was reduced as sodium was progressively replaced by choline, and ouabain inhibited uptake in the proximal convoluted tubules, but not in the thick ascending limbs. To a limited extent, lithium could replace sodium in the incubation medium with no depression of peroxidase uptake. These results suggest that a sodium-stimulated, ouabain-sensitive ATPase may be involved in the uptake of protein by cells of the kidney tubule. The intracellular transport of peroxidase in cells of the proximal convoluted tubules was abolished by cold, anoxia, and 2,4-dinitrophenol, but it was not affected by concentrations of ouabain which inhibited the uptake of the protein.

Familial Renal Tubular Dysgenesis: A Disorder Not Isolated to Proximal Convoluted Tubules

1995 ◽  
Vol 15 (6) ◽  
pp. 915-922 ◽  
Author(s):  
I. Ariel ◽  
T. R. Wells ◽  
B. H. Landing ◽  
M. Sagi ◽  
B. Bar-Oz ◽  
...  
Keyword(s):  
Renal Tubular Dysgenesis ◽  
Renal Tubular ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

scholarly journals THE USE OF SILVER NITRATE AS A VITAL STAIN, AND ITS DISTRIBUTION IN SEVERAL MAMMALIAN TISSUES AS STUDIED WITH THE ELECTRON MICROSCOPE

1955 ◽  
Vol 1 (2) ◽  
pp. 111-118 ◽  
Author(s):  
Edward W. Dempsey ◽  
George B. Wislocki
Keyword(s):  
Electron Microscope ◽  
Silver Nitrate ◽  
Chronic Administration ◽  
Pancreatic Acinar Cell ◽  
Basement Membranes ◽  
Metallic Silver ◽  
Hepatic Cells ◽  
Kidney Liver ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

After chronic administration of a dilute solution of silver nitrate in drinking water to rats, mice, and guinea pigs, granular deposits of metallic silver were detected in electron micrographs of the kidney, liver, thyroid, and pancreas. The silver deposits were in the form of extremely dense, angular particles with sharp outlines. They varied from aggregates a few microns in diameter down to granules at the limit of resolution of the electron microscope. The principal sites of deposition were (1) basement membranes, especially those of the renal glomeruli, proximal convoluted tubules, and various glands, and those associated with vascular endothelium, and (2) the cytoplasm of fixed and free macrophages. Both in Kupffer cells lining hepatic sinusoids and in the wandering macrophages of other tissues, the silver was segregated in discrete vacuoles. In addition, granular deposits were observed in occasional vesicular structures in the proximal convoluted tubules of the kidney, the hepatic cells, and the pancreatic acinar cell. These structures, in favorable preparations, contained an outer double layered membrane and internal folds similar to those of mitochondria, from which they appear to have been derived. The significance of these findings in heavy metal poisoning and in cellular physiology is briefly discussed.

Tubular organization and vascular-tubular relations in the dog kidney

1975 ◽  
Vol 229 (3) ◽  
pp. 695-713 ◽  
Author(s):  
R Beeuwkes ◽  
JV Bonventre
Keyword(s):  
Blood Vessels ◽  
Double Injection ◽  
Dog Kidney ◽  
Perfusion Fixation ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

Tubular organization and vascular-tubular relations were studied by double injection in canine kidneys. Blood vessels were injected via the artery after perfusion fixation. Tubules were injected by micropipettes inserted into the urinary spaces of selected glomeruli in cleared slices. One hundred proximal convoluted tubules, 16 Henle loops, and 5 distal convoluted tubules were defined. Only subcapsular proximal convolutions were perfused by efferent vessels arising from the same glomerulus (43 of 55). In midcortex, proximal convolutions were generally perfused over less than half their length by the parent efferent (21 of 31). Here tubules entirely perfused by the parent efferent were rare (2 of 31). No inner cortical proximal convolutions were perfused by the efferent from the same glomerulus (0 of 14). Henle's loops were found to be perfused by the efferents of many glomeruli regardless of the cortical position of the parent glomerulus. Distal convolutions shared the perfusion of proximal convolutions of the same glomerulus. Thus, each nephron is apparently functionally dependent on efferent blood from glomeruli of many other nephrons. New synoptic diagrams of canine renal organization are presented.

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