Patch clamp study on primary culture of isolated proximal convoluted tubules

1988 ◽  
Vol 413 (1) ◽  
pp. 51-61 ◽  
Author(s):  
J. Merot ◽  
M. Bidet ◽  
B. Gachot ◽  
S. Le Maout ◽  
M. Tauc ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Primary Culture ◽  
Clamp Study ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

Patch-clamp study of neurons and glial cells in isolated myenteric ganglia

2000 ◽  
Vol 278 (4) ◽  
pp. G644-G651 ◽  
Author(s):  
M. Hanani ◽  
M. Francke ◽  
W. Härtig ◽  
J. Grosche ◽  
A. Reichenbach ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Glial Cells ◽  
Muscle Layer ◽  
Membrane Receptors ◽  
Delayed Rectifier ◽  
Longitudinal Muscle Layer ◽  
Inward Currents ◽  
Morphological Identity ◽  
Clamp Study ◽  
Myenteric Ganglia

Most of the physiological information on the enteric nervous system has been obtained from studies on preparations of the myenteric ganglia attached to the longitudinal muscle layer. This preparation has a number of disadvantages, e.g., the inability to make patch-clamp recordings and the occurrence of muscle movements. To overcome these limitations we used isolated myenteric ganglia from the guinea pig small intestine. In this preparation movement was eliminated because muscle was completely absent, gigaseals were obtained, and whole cell recordings were made from neurons and glial cells. The morphological identity of cells was verified by injecting a fluorescent dye by micropipette. Neurons displayed voltage-gated inactivating inward Na+ and Ca2+currents as well as delayed-rectifier K+ currents. Immunohistochemical staining confirmed that most neurons have Na+ channels. Neurons responded to GABA, indicating that membrane receptors were retained. Glial cells displayed hyperpolarization-induced K+ inward currents and depolarization-induced K+ outward currents. Glia showed large “passive” currents that were suppressed by octanol, consistent with coupling by gap junctions among these cells. These results demonstrate the advantages of isolated ganglia for studying myenteric neurons and glial cells.

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