The irreversibility of inner mitochondrial membrane permeabilization by Ca2+ plus prooxidants is determined by the extent of membrane protein thiol cross-linking

1996 ◽  
Vol 28 (6) ◽  
pp. 523-529 ◽  
Author(s):  
Roger F. Castilho ◽  
Alicia J. Kowaltowski ◽  
Anibal E. Vercesi
Keyword(s):  
Membrane Protein ◽  
Mitochondrial Membrane ◽  
Membrane Permeabilization ◽  
Cross Linking ◽  
Inner Mitochondrial Membrane ◽  
Protein Thiol ◽  
Mitochondrial Membrane Permeabilization

scholarly journals Mitochondrial respiratory chain inhibitors modulate the metal-induced inner mitochondrial membrane permeabilization.

2010 ◽  
Vol 57 (4) ◽  
Author(s):  
Elena A Belyaeva
Keyword(s):  
Heavy Metal ◽  
Mitochondrial Membrane ◽  
Molecular Mechanisms ◽  
Protective Action ◽  
Membrane Permeabilization ◽  
Mitochondrial Swelling ◽  
Complex Iii ◽  
Rat Liver Mitochondria ◽  
Inner Mitochondrial Membrane ◽  
Mitochondrial Membrane Permeabilization

To elucidate the molecular mechanisms of the protective action of stigmatellin (an inhibitor of complex III of mitochondrial electron transport chain, mtETC) against the heavy metal-induced cytotoxicity, we tested its effectiveness against mitochondrial membrane permeabilization produced by heavy metal ions Cd²(+), Hg²(+), Cu²(+) and Zn²(+), as well as by Ca²(+) (in the presence of P(i)) or Se (in form of Na₂SeO₃) using isolated rat liver mitochondria. It was shown that stigmatellin modulated mitochondrial swelling produced by these metals/metalloids in the isotonic sucrose medium in the presence of ascorbate plus tetramethyl-p-phenylenediamine (complex IV substrates added for energization of the mitochondria). It was found that stigmatellin and other mtETC inhibitors enhanced the mitochondrial swelling induced by selenite. However, in the same medium, all the mtETC inhibitors tested as well as cyclosporin A and bongkrekic acid did not significantly affect Cu²(+)-induced swelling. In contrast, the high-amplitude swelling produced by Cd²(+), Hg²(+), Zn²(+), or Ca²(+) plus P(i) was significantly depressed by these inhibitors. Significant differences in the action of these metals/metalloids on the redox status of pyridine nucleotides, transmembrane potential and mitochondrial respiration were also observed. In the light of these results as well as the data from the recent literature, our hypothesis on a possible involvement of the respiratory supercomplex, formed by complex I (P-site) and complex III (S-site) in the mitochondrial permeabilization mediated by the mitochondrial transition pore, is updated.

scholarly journals Dynamics of outer mitochondrial membrane permeabilization during apoptosis

2009 ◽  
Vol 16 (4) ◽  
pp. 613-623 ◽  
Author(s):  
M Rehm ◽  
H J Huber ◽  
C T Hellwig ◽  
S Anguissola ◽  
H Dussmann ◽  
...  
Keyword(s):  
Mitochondrial Membrane ◽  
Membrane Permeabilization ◽  
Outer Mitochondrial Membrane ◽  
Mitochondrial Membrane Permeabilization
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