Comparison of three nonradioactive and a radioactive DNA probe for the detection of target DNA by DNA hybridization

1991 ◽  
Vol 22 (2) ◽  
pp. 79-84 ◽  
Author(s):  
Lawrence R. Zeph ◽  
Xiaoyan Lin ◽  
Guenther Stotzky
Keyword(s):  
Dna Hybridization ◽  
Dna Probe ◽  
Target Dna

Rapid DNA hybridization based on ac field focusing of magnetically labeled target DNA

2005 ◽  
Vol 87 (1) ◽  
pp. 013901 ◽  
Author(s):  
H. A. Ferreira ◽  
N. Feliciano ◽  
D. L. Graham ◽  
L. A. Clarke ◽  
M. D. Amaral ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Target Dna ◽  
Ac Field

scholarly journals Label-Free Dengue Detection Utilizing PNA/DNA Hybridization Based on the Aggregation Process of Unmodified Gold Nanoparticles

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Samsulida Abdul Rahman ◽  
Rafidah Saadun ◽  
Nur Ellina Azmi ◽  
Nurhayati Ariffin ◽  
Jaafar Abdullah ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Peptide Nucleic Acid ◽  
Virus Detection ◽  
Label Free ◽  
Color Changes ◽  
Phosphate Backbone ◽  
Single Base Mismatch ◽  
Target Dna ◽  
Base Mismatch

A label-free optical detection method based on PNA/DNA hybridization using unmodified gold nanoparticles (AuNPs) for dengue virus detection has been successfully developed. In this study, no immobilization method is involved and the hybridization of PNA/DNA occurs directly in solution. Unmodified AuNPs undergo immediate aggregation in the presence of neutral charge peptide nucleic acid (PNA) due to the coating of PNA on AuNPs surface. However, in the presence of complementary targets DNA, the hybridization of PNA probe with target DNA forms negatively charged complexes due to the negatively charged phosphate backbone of the target DNA. The negatively charged complexes adsorbed onto the AuNPs surface ensure sufficient charge repulsion, need for AuNPs dispersion, and stability in solution. The detection procedure is a naked eye method based on immediate color changes and also through UV-vis adsorption spectra. The selectivity of the proposed method was studied successfully by single base mismatch and noncomplementary target DNA.

scholarly journals DNA Hybridization Detection Based on Resonance Frequency Readout in Graphene on Au SPR Biosensor

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Md. Biplob Hossain ◽  
Md. Masud Rana
Keyword(s):  
Resonance Frequency ◽  
Dna Hybridization ◽  
Biomolecular Recognition ◽  
Nucleotide Polymorphisms ◽  
Mismatched Dna ◽  
Spr Sensor ◽  
Spr Biosensor ◽  
Target Dna ◽  
Dna Strands ◽  
Recognition Elements

This paper demonstrates a numerical modeling of surface plasmon resonance (SPR) biosensor for detecting DNA hybridization by recording the resonance frequency characteristics (RFC). The proposed sensor is designed based on graphene material as biomolecular recognition elements (BRE) and the sharp SPR curve of gold (Au). Numerical analysis shows that the variation of RFC for mismatched DNA strands is quiet negligible whereas that for complementary DNA strands is considerably countable. Here, graphene is used to perform faster immobilization between target DNA and probe DNA. The usage of graphene also changes the RFC that ensure hybridization of DNA event by utilizing its optochemical property. In addition, proposed sensor successfully distinguishes between hybridization and single-nucleotide polymorphisms (SNP) by observing the variation level of RFC and maximum transmittance. Therefore, the proposed frequency readout based SPR sensor could potentially open a new window of detection for biomolecular interactions. We also highlight the advantage of using graphene sublayer by performing the sensitivity analysis. Sandwiching of each graphene sublayer enhances 95% sensitivity comparing with conventional SPR sensor.

Two-Surface Strategy in Electrochemical DNA Hybridization Assays: Detection of Osmium-Labeled Target DNA at Carbon Electrodes

2003 ◽  
Vol 15 (5-6) ◽  
pp. 431-440 ◽  
Author(s):  
Miroslav Fojta ◽  
Ludek Havran ◽  
Sabina Billova ◽  
Pavel Kostecka ◽  
Michal Masarik ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Carbon Electrodes ◽  
Target Dna ◽  
Hybridization Assays

Quantum Dot-Bead-DNA Probe-Based Hybridization Fluorescence Assays on Microfluidic Chips

2015 ◽  
Vol 15 (10) ◽  
pp. 7918-7921 ◽  
Author(s):  
Seshadri Reddy Ankireddy ◽  
Jongsung Kim
Keyword(s):  
Quantum Dot ◽  
Simultaneous Detection ◽  
Dna Probe ◽  
Detection Methods ◽  
Microfluidic Chips ◽  
Wild Type ◽  
Research Focus ◽  
Target Dna ◽  
Type Sequence ◽  
Hybridization Detection

The development of chip-based, quantum dot (QD)-bead-DNA conjugate probes for hybridization detection is a prime research focus in the field of microfluidics. QD-Bead-DNA probe-based hybridization detection methods are often called “bead-based assays,” and their success is substantially influenced by the dispensing and manipulation capabilities of microfluidic technology. Met was identified as a prognostic marker in different cancers including lung, renal, liver, head and neck, stomach, and breast. In this report, the cancer causing Met gene was detected with QDs attached to polystyrene microbeads. We constructed a microfluidic platform using a flexible PDMS polymer. The chip consists of two channels, with two inlets and two outlets. The two channels were integrated with QD-bead-DNA probes for simultaneous detection of wild type target DNA and mutant DNA, containing three nucleotide changes compared to the wild type sequence. The fluorescence quenching ability of QDs within the channels of microfluidic chips were compared for both DNAs.

scholarly journals Use of a Nonradioactive DNA Probe for Detection of Anaplasma Marginale Infection in Field Cattle: Comparison with Complement Fixation Serology and Microscopic Examination

1997 ◽  
Vol 9 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Nie-Lin Ge ◽  
Katherine M. Kocan ◽  
Sidney A. Ewing ◽  
Edmour F. Blouin ◽  
Wanda L. Edwards ◽  
...  
Keyword(s):  
Microscopic Examination ◽  
Dna Hybridization ◽  
Complement Fixation ◽  
Blot Hybridization ◽  
Anaplasma Marginale ◽  
Beef Cows ◽  
Dna Probe ◽  
Hybridization Assay ◽  
Slot Blot ◽  
Nylon Membrane

A sensitive Anaplasma marginale-specific 409-base pair DNA probe was developed in a previous study for detection of A. marginale infection in experimentally infected cattle with a test that employed slot-blot and in situ hybridization. To test the suitability of the probe to detect A. marginale in the blood of naturally infected carrier cattle, slot-blot hybridization was used to determine the infection rate of A. marginale in cattle from 3 geographic areas in Oklahoma. For comparison, blood samples from the same cattle were also examined by light microscopy and were tested by the complement fixation test. For the DNA hybridization assay, the probe was labeled with digoxigenin 11-dUTP by polymerase chain reaction (PCR). DNA was extracted from blood using the QIAamp blood kit and then applied to a nylon membrane and hybridized with the probe. The study herds consisted of 31 beef cows in Harper County, OK, and 42 and 70 dairy cows from Payne and Pittsburg counties, OK, respectively. In the 3 herds, 80.6%, 92.8%, and 57.1% of the cows were positive for A. marginale as assessed with the DNA hybridization assay. In contrast, only 25.8% and 2.86% were complement fixation positive in 2 herds, and no complement fixation positives were found in 1 herd. Uncountable parasitemia that was too low to accurately determine (< 0.01%) from 29.0%, 4.8%, and 11.4% of the samples, respectively, was demonstrated by microscopic examination. All samples positive by complement fixation and microscopic examination had positive probe reactions in the DNA hybridization assay. Therefore, the PCR-mediated nonradioactive DNA probe described here may be useful in epidemiologic investigations and in identification of carrier cattle. This assay could be adapted for use in diagnostic laboratories because it is sensitive, specific, nontoxic, quickly executed, and inexpensive.

Immobilization of single stranded DNA probe onto polypyrrole-polyvinyl sulfonate for application to DNA hybridization biosensor

2007 ◽  
Vol 126 (2) ◽  
pp. 655-663 ◽  
Author(s):  
Kavita Arora ◽  
Nirmal Prabhakar ◽  
Subhash Chand ◽  
B.D. Malhotra
Keyword(s):  
Dna Hybridization ◽  
Dna Probe ◽  
Single Stranded Dna

Absence of the J genome in Leymus species (Poaceae: Triticeae): evidence from DNA hybridization and meiotic pairing

Genome ◽  
1994 ◽  
Vol 37 (2) ◽  
pp. 231-235 ◽  
Author(s):  
Richard R.-C. Wang ◽  
Kevin B. Jensen
Keyword(s):  
Dna Hybridization ◽  
Meiotic Chromosome ◽  
Dna Probe ◽  
Triploid Hybrid ◽  
Meiotic Pairing ◽  
Psathyrostachys Juncea ◽  
Chromosome Associations ◽  
Mother Cells ◽  
Thinopyrum Elongatum ◽  
Cell Chromosome

To test the presence of a J genome in the type species of Leymus, L. arenarius, its total genomic DNA and that of tetraploids L. mollis, L. salinus ssp. salmonis, L. ambiguus, L. chinensis, L. secalinus, L. alaicus ssp. karataviensis, and L. innovatus were probed with the 277-bp insert of pLeUCD2, which can hybridize with the J, S, and P but not with the N, R, V, Q, I, T, and ABD genomes. The DNA probe hybridized with PalI- or TaqI-digested total DNAs from Thinopyrum elongatum (JeJe diploid) and T. elongatum × Psathyrostachys juncea (JeN hybrid), but not with those from L. arenarius (NNNNXXXX octoploid) and all tetraploid Leymus species (NNXX). Attempts to cross diploid Thinopyrum and tetraploid Leymus species yielded only one triploid hybrid, T. elongatum × L. salinus ssp. salmonis. Meiotic chromosome associations at metaphase I of pollen mother cells in the triploid hybrid averaged 19.69 univalents, 0.64 bivalents, and 0.01 trivalents per cell. Chromosome pairings in the tetraploid hybrids of L. mollis × L. salinus ssp. salmonis, and the reciprocal cross, indicate that L. mollis and L. salinus ssp. salmonis shae the same genomic constitution. Both the DNA probe and genome analysis results confirm the absence of the J genome in the seven additional Leymus species tested. Meiotic data indicated that tetraploid Leymus species could not have the genome formula N1N1N2N2; thus their genome formulas should remain as NNXX until the source of X is identified.Key words: genome, Leymus, DNA probe, Southern blot, meiotic pairing, hybrid.

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