Flocculation of lag phase yeast cells ofCandida albicans

1964 ◽  
Vol 30 (1) ◽  
pp. 401-411 ◽  
Author(s):  
D. E. Bianchi
Keyword(s):  
Yeast Cells ◽  
Lag Phase ◽  
Ofcandida Albicans

The effects of starvation and acidification on lag phase duration of surviving yeast cells

2018 ◽  
Vol 275 ◽  
pp. 60-64 ◽  
Author(s):  
Kenichi Shibata ◽  
Kohei Obase ◽  
Kiminori Itoh ◽  
Takashi Amemiya
Keyword(s):  
Yeast Cells ◽  
Lag Phase ◽  
Phase Duration

scholarly journals Effects of Low-Shear Modeled Microgravity on Cell Function, Gene Expression, and Phenotype in Saccharomyces cerevisiae

2006 ◽  
Vol 72 (7) ◽  
pp. 4569-4575 ◽  
Author(s):  
B. Purevdorj-Gage ◽  
K. B. Sheehan ◽  
L. E. Hyman
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Cell Function ◽  
Yeast Cells ◽  
Lag Phase ◽  
Limited Information ◽  
Cellular Functions ◽  
Modeled Microgravity ◽  
Expression Of Genes ◽  
Low Shear

ABSTRACT Only limited information is available concerning the effects of low-shear modeled microgravity (LSMMG) on cell function and morphology. We examined the behavior of Saccharomyces cerevisiae grown in a high-aspect-ratio vessel, which simulates the low-shear and microgravity conditions encountered in spaceflight. With the exception of a shortened lag phase (90 min less than controls; P < 0.05), yeast cells grown under LSMMG conditions did not differ in growth rate, size, shape, or viability from the controls but did differ in the establishment of polarity as exhibited by aberrant (random) budding compared to the usual bipolar pattern of controls. The aberrant budding was accompanied by an increased tendency of cells to clump, as indicated by aggregates containing five or more cells. We also found significant changes (greater than or equal to twofold) in the expression of genes associated with the establishment of polarity (BUD5), bipolar budding (RAX1, RAX2, and BUD25), and cell separation (DSE1, DSE2, and EGT2). Thus, low-shear environments may significantly alter yeast gene expression and phenotype as well as evolutionary conserved cellular functions such as polarization. The results provide a paradigm for understanding polarity-dependent cell responses to microgravity ranging from pathogenesis in fungi to the immune response in mammals.

scholarly journals A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Abbas Jariani ◽  
Lieselotte Vermeersch ◽  
Bram Cerulus ◽  
Gemma Perez-Samper ◽  
Karin Voordeckers ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Mammalian Cells ◽  
Yeast Cells ◽  
Lag Phase ◽  
Rna Seq ◽  
Molecular Processes ◽  
Sequencing Technology ◽  
Single Cell Rna Sequencing ◽  
Order Of Magnitude

Current methods for single-cell RNA sequencing (scRNA-seq) of yeast cells do not match the throughput and relative simplicity of the state-of-the-art techniques that are available for mammalian cells. In this study, we report how 10x Genomics’ droplet-based single-cell RNA sequencing technology can be modified to allow analysis of yeast cells. The protocol, which is based on in-droplet spheroplasting of the cells, yields an order-of-magnitude higher throughput in comparison to existing methods. After extensive validation of the method, we demonstrate its use by studying the dynamics of the response of isogenic yeast populations to a shift in carbon source, revealing the heterogeneity and underlying molecular processes during this shift. The method we describe opens new avenues for studies focusing on yeast cells, as well as other cells with a degradable cell wall.

Changes of trehalose content and expression of relative genes during the bioethanol fermentation bySaccharomyces cerevisiae

2016 ◽  
Vol 62 (10) ◽  
pp. 827-835 ◽  
Author(s):  
Chenfeng Yi ◽  
Fenglian Wang ◽  
Shijun Dong ◽  
Hao Li
Keyword(s):  
Phase Transition ◽  
Stationary Phase ◽  
Ethanol Tolerance ◽  
Stationary Phases ◽  
Exponential Phase ◽  
Yeast Cells ◽  
Ethanol Stress ◽  
Lag Phase ◽  
Significant Difference ◽  
Trehalose Content

Traditionally, trehalose is considered as a protectant to improve the ethanol tolerance of Saccharomyces cerevisiae. In this study, to clarify the changes and roles of trehalose during the bioethanol fermentation, trehalose content and expression of related genes at lag, exponential, and stationary phases (i.e., 2, 8, and 16 h of batch fermentation process) were determined. Although yeast cells at exponential and stationary phase had higher trehalose content than cells at lag phase (P < 0.01), there was no significant difference in trehalose content between exponential and stationary phases (P > 0.05). Moreover, expression of the trehalose degradation-related genes NTH1 and NTH2 decreased at exponential phase in comparison with that at lag phase; compared with cells at lag phase, cells at stationary phase had higher expression of TPS1, ATH1, NTH1, and NTH2 but lower expression of TPS2. During the lag–exponential phase transition, downregulation of NTH1 and NTH2 promoted accumulation of trehalose, and to some extent, trehalose might confer ethanol tolerance to S. cerevisiae before stationary phase. During the exponential–stationary phase transition, upregulation of TPS1 contributed to accumulation of trehalose, and Tps1 protein might be indispensable in yeast cells to withstand ethanol stress at the stationary phase. Moreover, trehalose would be degraded to supply carbon source at stationary phase.

Inefficient delivery of yeast cells as an explanation for reduced plating efficiency ofCandida albicans

2002 ◽  
Vol 40 (5) ◽  
pp. 465-469 ◽  
Author(s):  
M. A. Bhatti ◽  
J. Hjertstedt ◽  
B. L. Hahn ◽  
P. G. Sohnle
Keyword(s):  
Plating Efficiency ◽  
Yeast Cells ◽  
Ofcandida Albicans

Significance of the lag phase in K1 killer toxin action on sensitive yeast cells

1995 ◽  
Vol 40 (2) ◽  
pp. 213-215 ◽  
Author(s):  
H. Kurzweilová ◽  
K. Sigler
Keyword(s):  
Killer Toxin ◽  
Yeast Cells ◽  
Lag Phase

Effect of Acids and Sorbate Combinations on the Growth of Four Osmophilic Yeasts

1982 ◽  
Vol 45 (12) ◽  
pp. 1138-1142 ◽  
Author(s):  
LAWRENCE RESTAINO ◽  
LAWRENCE M. LENOVICH ◽  
STEPHANIE BILLS
Keyword(s):  
Growth Rate ◽  
Growth Medium ◽  
The Other ◽  
Yeast Cells ◽  
Potassium Sorbate ◽  
Lag Phase ◽  
Potato Dextrose Broth ◽  
Yeast Strains ◽  
Antimycotic Agent ◽  
Antimicrobial Effectiveness

The effects of hydrochloric, citric, lactic, phosphoric and malic acids in combination with potassium sorbate on the growth of Saccharomyces bailii, Saccharomyces acidifaciens (Saccharomyces bailii var. osmophilus), Saccharomyces rouxii and Saccharomyces bisporus were evaluated. Double strength potato dextrose broth supplemented with 58% (wt/vol) sucrose, 14% (wt/vol) glucose, and 0.2% agar acidulated to a pH of 5.0 to a final aw of 0.88 to 0.89 was used as the growth medium. In general, at 0.05% potassium sorbate, S. rouxii and S. bisporus were more resistant than S. bailii and S. acidifaciens to the antimycotic agent independent of the acid used to acidulate the growth medium, whereas 0.1% potassium sorbate inhibited the growth of the four yeast strains. At 0.05% potassium sorbate, growth occurred (1 log number yeast cells/ml) for S. acidifaciens in the lactic acid/sorbate combination after 36 h of incubation, whereas a bacteriostatic relationship existed for the other acids employed. Citric acid potentiated the antimicrobial effectiveness of 0.05% potassium sorbate at pH 5.0 against the growth of S. rouxii and S. bisporus by either delaying the lag phase or reducing the growth rate.

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