Continuous measurement of plasma protein content in the alert rat during and after 3 to 5 minutes of moderate activity

1986 ◽  
Vol 81 (6) ◽  
pp. 558-566 ◽  
Author(s):  
H. -J. Mösinger ◽  
O. Aziz ◽  
H. O. Lindt
Keyword(s):  
Protein Content ◽  
Plasma Protein ◽  
Continuous Measurement ◽  
Moderate Activity ◽  
Alert Rat

scholarly journals THE INFLUENCE OF DIFFERENT MOLECULAR WEIGHT SEMINAL PLASMA PROTEIN CONTENT ON SOME FERTILITY PARAMETERS IN BOAR'S EJACULATES

2019 ◽  
Vol 56 (4) ◽  
Author(s):  
Ivan Blagoje Stančić ◽  
Igor Zdraveski ◽  
Saša Dragin ◽  
Jelena Blagoje Apić ◽  
Slobodanka Vakanjac ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Protein Content ◽  
Plasma Protein ◽  
Seminal Plasma ◽  
Different Molecular Weight

scholarly journals RECIPROCAL CHANGES IN PLASMA PROTEIN AND PLASMA ACACIA AS RESULT OF HIGH AND LOW PROTEIN DIETS

1950 ◽  
Vol 91 (4) ◽  
pp. 425-431 ◽  
Author(s):  
R. E. Knutti ◽  
J. B. Goetsch ◽  
R. A. Warrick
Keyword(s):  
Protein Content ◽  
Plasma Protein ◽  
Protein Concentration ◽  
High Protein Diet ◽  
The Body ◽  
Protein Diet ◽  
Plasma Protein Concentration ◽  
Gum Acacia ◽  
Normal Limits ◽  
Low Protein

Dogs were made hypoproteinemic by repeated injections of gum acacia, and the acacia injections were discontinued. Diets of varying protein content were then given. When a high protein diet is provided the plasma protein concentration increases; with a low protein diet, or under conditions of fasting, the plasma protein concentration diminishes. Similarly, plasma acacia concentration shows increases and decreases which are reciprocal to the protein variations. Total circulating plasma protein and total circulating plasma acacia show similar changes. In all instances total circulating colloid (acacia plus protein) concentration adds up to an amount within normal limits for protein alone. The results indicate that under these conditions, acacia stored in the body (principally in the liver) can be removed from its site of deposit and returned to the blood. The data also show that dogs in which acacia is deposited in large quantities, require a larger amount of protein in the diet to maintain a constant plasma protein content than do normal dogs. It appears that the mechanism for maintenance of peripheral colloidal material may be dependent on differences in intracellular and extracellular colloidal osmotic pressure. The experiments also support the idea that plasma protein molecules, as well as gum acacia, may pass in and out of cells through the cell membranes.

scholarly journals Developmental Changes in the Late Larva of Calliphora Stygia Iv. Uptake of Plasma Protein by the Fat Body

1971 ◽  
Vol 24 (2) ◽  
pp. 291 ◽  
Author(s):  
Marjoryd Martin ◽  
Judith F Kinnear ◽  
JA Thomson
Keyword(s):  
Protein Content ◽  
Plasma Protein ◽  
Protein Concentration ◽  
Fat Body ◽  
Developmental Changes

The protein content of the larval fat body of O. stygia increases during late third-instar development from 1 �0 mg at day 7 to 3�4 mg in the white prepupa (day 11). This increase is due predominantly to uptake of protein from the haemolymph, in which there is a concurrent fall in protein concentration. The rate of uptake rises from 0�6 mg/day at days 8-9 to 0�9 mg/day at days 10-11.

Continuous measurements of plasma protein extravasation with microdialysis after various inflammatory challenges in rat and mouse skin

2004 ◽  
Vol 286 (1) ◽  
pp. H108-H112 ◽  
Author(s):  
V. V. Iversen ◽  
A. Brønstad ◽  
E.-A. B. Gjerde ◽  
R. K. Reed
Keyword(s):  
Intravenous Injection ◽  
Plasma Protein ◽  
Continuous Measurement ◽  
Drug Application ◽  
Mouse Skin ◽  
Cremophor El ◽  
Protein Extravasation ◽  
Continuous Measurements ◽  
Plasma Protein Extravasation ◽  
Rats And Mice

This study describes the use of microdialysis technique for continuous measurement of plasma protein extravasation (PPE) in rat and mouse skin with drug application either intravenously or via the microdialysis fiber. Hollow plasmapheresis fibers (3-cm length, 0.4-mm diameter, cutoff 3,000 kDa) were placed subcutaneously on the back of anesthetized mice and rats. Intravenous injection of dextran (Macrodex, 60 mg/ml) increased PPE by 355% from baseline within 30 min in rats with ligated kidneys ( n = 6; P < 0.05) but not in animals with intact kidneys. Phalloidin (500 μg/kg iv 40 min before dextran, n = 6; P < 0.05) did not change the response to dextran in either group. Animals receiving PGE1, compound 48/80 (mice), paclitaxel, docetaxel, and cremophor EL via the microdialysis fiber were also provided with a control fiber receiving vehicle. Both rats and mice had constant PPE in the control fiber, and there was no change in PPE in the NaCl-treated groups (rats, n = 4; mice, n = 6). Application via the fiber of PGE1 (20 μg/ml), compound 48/80 (mice; 4 mg/ml), and docetaxel (0.5 mg/ml) increased PPE compared with baseline within 60 min by 139% ( n = 6; P < 0.05), 273% ( n = 6; P < 0.05), and 325% ( n = 5; P < 0.05), respectively. Phalloidin alone did not increase PPE ( n = 5; P < 0.05). Pretreatment with phalloidin did not inhibit the increase after PGE1 or compound 48/80 but inhibited that after docetaxel ( n = 6). Paclitaxel (0.6 mg/ml, n = 5) or vehicle (Cremophor) ( n = 5) gave no increase in PPE. The results demonstrate that microdialysis can be used to continuously measure changes in PPE after inflammatory challenges in skin of rats and mice.

scholarly journals BLOOD PLASMA PROTEIN PRODUCTION AS INFLUENCED BY AMINO ACIDS

1939 ◽  
Vol 69 (5) ◽  
pp. 721-738 ◽  
Author(s):  
S. C. Madden ◽  
W. A. Noehren ◽  
G. S. Waraich ◽  
G. H. Whipple
Keyword(s):  
Blood Plasma ◽  
Red Blood Cells ◽  
Protein Content ◽  
Plasma Protein ◽  
Blood Cells ◽  
Plasma Proteins ◽  
Protein Production ◽  
Basal Diet ◽  
Liver Protein ◽  
Clinically Normal

When blood plasma proteins are depleted by bleeding with return of the washed red blood cells (plasmapheresis) it is possible to bring dogs to a steady state of hypoproteinemia and a uniform plasma protein production on a basal low protein diet. These dogs are clinically normal. By the introduction of variables into their standardized existence insight into the formation of plasma proteins can be obtained. The liver basal diet maintains health in such hypoproteinemic dogs during periods as long as a year. 17 to 27 per cent of its protein content (entirely liver protein) is presumably converted into plasma protein. Gelatin alone added to the liver basal diet causes very little if any extra plasma protein production. The addition to gelatin of cystine, or tyrosine, or tryptophane, or of both tyrosine and tryptophane has little or no effect on its potency for plasma protein production. When gelatin is supplemented by cystine and either tryptophane or tyrosine, 25 to 40 per cent of the protein content of the combination is converted into plasma protein—an efficiency equaling that of any protein hitherto tested. Preliminary experiments indicate that methionine cannot substitute for cystine nor can phenylalanine substitute for tyrosine in the efficient combination of gelatin plus cystine plus tyrosine. Laked red blood cells given by vein afford little or no material for plasma protein formation. When the reserve stores of plasma protein building material are exhausted the dog can form little if any plasma protein during protein-free diet periods.

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