Cytokines modulate IgE-mediated histamine liberation from human basophils

1992 ◽  
Vol 36 (S2) ◽  
pp. C260-C264 ◽  
Author(s):  
K. S. Kristensen ◽  
K. Bendtzen ◽  
S. Norn ◽  
P. Stahl Skov ◽  
M. Pedersen ◽  
...  
Keyword(s):  
Histamine Liberation ◽  
Ige Mediated ◽  
Human Basophils

scholarly journals Enhancement of IgE-mediated histamine release from human basophils by viruses: role of interferon.

1977 ◽  
Vol 145 (4) ◽  
pp. 892-906 ◽  
Author(s):  
S Ida ◽  
J J Hooks ◽  
R P Siraganian ◽  
A L Notkins
Keyword(s):  
Tissue Culture ◽  
Antiviral Activity ◽  
Histamine Release ◽  
Influenza A ◽  
Culture Fluid ◽  
Soluble Factor ◽  
Release Histamine ◽  
Ige Mediated ◽  
Human Basophils

Human leukocytes maintained in culture are induced to release histamine when exposed to ragweed antigen E or anti-IgE. Leukocyte cultures incubated with virus (i.e. HSV-1, Influenza A, and Adeno-1) but not exposed to ragweed antigen E or anti-IgE fail to release histamine. If, however, leukocyte cultures are first exposed to virus and then to ragweed antigen E or anti-IgE, significant enhancement of histamine release occurs. Both infectious and inactivated virus enhance histamine release and the degree of enhancement is related to the concentration of virus and the length of the incubation. Tissue culture fluid harvested 8 h after exposure of leukocytes to virus contains a soluble factor which is capable of enhancing histamine release when added to fresh leukocyte cultures. This factor has all the properties of interferon including species specificity and cannot be dissociated from the antiviral activity of interferon. Moreover, both known inducers of interferon (poly I:poly C) and standard preparations of interferon are capable of enhancing histamine release. The enhancement of histamine release by interferon represents a new biological role for interferon.

Superallergens: a novel mechanism of IgE-mediated activation of human basophils and mast cells

2004 ◽  
Vol 4 (s2) ◽  
pp. 64-75 ◽  
Author(s):  
G. Marone ◽  
F. W. Rossi ◽  
M. Bova ◽  
A. Detoraki ◽  
B. Liccardo ◽  
...  
Keyword(s):  
Mast Cells ◽  
Ige Mediated ◽  
Human Basophils

LAMP1 and CD63 Expression In Mouse Mast Cells and Human Basophils Rendered Hyporesponsive By Antigen/IgE-Mediated Activation and Desensitization

2014 ◽  
Vol 133 (2) ◽  
pp. AB59 ◽  
Author(s):  
Pedro Giavina-Bianchi ◽  
Matthieu Picard ◽  
Joana Caiado ◽  
Veronica Mezzano ◽  
Mariana C. Castells
Keyword(s):  
Mast Cells ◽  
Ige Mediated ◽  
Human Basophils ◽  
Cd63 Expression

scholarly journals The relationship between released soluble FceRI-alpha and its cell surface density on human basophils

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245942
Author(s):  
Donald MacGlashan
Keyword(s):  
Molecular Weight ◽  
Flow Cytometry ◽  
Cell Surface ◽  
Surface Density ◽  
Alpha Subunit ◽  
Constant Amount ◽  
Western Blots ◽  
Ige Mediated ◽  
The Relationship ◽  
Human Basophils

Background The IgE-mediated activation of mast cells and basophils results in the secretion of many substances, including the release of FceRI-alpha subunit. This released alpha subunit can bind IgE and it may act as a down-regulator of subsequent IgE-dependent reactions. However, previous studies do not observe loss of the mass of FceRI-alpha associated with the cells, at least not for human basophils. This study was designed to understand the basis for the discordant observations. Methods Purified human basophils were stimulated with multiple activating secretagogues and supernatants were examined for histamine and released FceRI-alpha. In addition, cell surface IgE densities (occupied and unoccupied) were measured by flow cytometry and total cellular content of mature and immature FceRI-alpha determined with Western blots. Results Released FceRI-alpha, on average, represented 7% of the total surface FceRI before the reaction. The molecular weight of the soluble FceRI-alpha was approximately 54 kD, larger than immature subunit and somewhat smaller than surface subunit. In addition, 1) release ceased long before internalized FceRI-alpha was processed, 2) release was insensitive to Bafilomycin A, 3) release was independent of the starting density of FceRI and 4) release occurred more effectively with non-IgE-dependent stimuli, FMLP or C5a. Conclusions There appears to be relatively constant amount of nearly mature FceRI-alpha that is susceptible to secretion events induced by any form of stimulation. The amount, on average, represents about 7% of the mature form of FceRI-alpha.

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