Chenopodium quinoa, a herbaceous test plant for chlorotic leaf spot virus in apple

1968 ◽  
Vol 74 (1) ◽  
pp. 12-16 ◽  
Author(s):  
Hillegonda J. Pfaeltzer
Keyword(s):  
Leaf Spot ◽  
Chenopodium Quinoa ◽  
Test Plant ◽  
Spot Virus ◽  
Chlorotic Leaf

An improved procedure for the purification of apple chlorotic leaf spot virus

1992 ◽  
Vol 72 (3) ◽  
pp. 937-941
Author(s):  
Delano James ◽  
Paul L. Monette
Keyword(s):  
Density Gradient ◽  
Leaf Spot ◽  
Gradient Centrifugation ◽  
Chenopodium Quinoa ◽  
Sucrose Density Gradient ◽  
High Yield ◽  
Spot Virus ◽  
Virus Particles ◽  
Intact Virus ◽  
Chlorotic Leaf

A reproducible procedure is described for the purification of apple chlorotic leaf spot virus (CLSV) from Chenopodium quinoa. Two cycles of sucrose density-gradient centrifugation in a magnesium-containing buffer were used. A relatively high yield of virus particles was obtained with little degradation observed. The average yield was 2.3 mg 100 g−1 fresh leaf tissue. The modal lengths of purified virus particles and particles from leaf sap were 692 nm and 704 nm, respectively.Key words: Chenopodium quinoa, sucrose density gradient, magnesium-containing buffer, intact virus

scholarly journals Characterization of Apricot pseudo-chlorotic leaf spot virus, A Novel Trichovirus Isolated from Stone Fruit Trees

2005 ◽  
Vol 95 (4) ◽  
pp. 420-426 ◽  
Author(s):  
D. Liberti ◽  
A. Marais ◽  
L. Svanella-Dumas ◽  
M. J. Dulucq ◽  
D. Alioto ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Chenopodium Quinoa ◽  
Fruit Trees ◽  
Open Reading Frames ◽  
Spot Virus ◽  
Phylogenetic Reconstructions ◽  
Wide Geographical Distribution ◽  
Chlorotic Leaf ◽  
Reading Frames

A trichovirus closely related to Apple chlorotic leaf spot virus (ACLSV) was detected in symptomatic apricot and Japanese plum from Italy. The Sus2 isolate of this agent cross-reacted with anti-ACLSV polyclonal reagents but was not detected by broad-specificity anti- ACLSV monoclonal antibodies. It had particles with typical trichovirus morphology but, contrary to ACLSV, was unable to infect Chenopodium quinoa and C. amaranticolor. The sequence of its genome (7,494 nucleotides [nt], missing only ≈30 to 40 nt of the 5′ terminal sequence) and the partial sequence of another isolate were determined. The new virus has a genomic organization similar to that of ACLSV, with three open reading frames coding for a replication-associated protein (RNA-dependent RNA polymerase), a movement protein, and a capsid protein, respectively. However, it had only ≈65 to 67% nucleotide identity with sequenced isolates of ACLSV. The differences in serology, host range, genome sequence, and phylogenetic reconstructions for all viral proteins support the idea that this agent should be considered a new virus, for which the name Apricot pseudo-chlorotic leaf spot virus (APCLSV) is proposed. APCLSV shows substantial sequence variability and has been recovered from various Prunus sources coming from seven countries, an indication that it is likely to have a wide geographical distribution.

scholarly journals Mapping the RNA-binding domain on the Apple chlorotic leaf spot virus movement protein

2005 ◽  
Vol 86 (1) ◽  
pp. 225-229 ◽  
Author(s):  
Masamichi Isogai ◽  
Nobuyuki Yoshikawa
Keyword(s):  
Leaf Spot ◽  
Rna Binding ◽  
Movement Protein ◽  
Binding Domain ◽  
Sequence Specificity ◽  
Nucleic Acid Binding ◽  
Spot Virus ◽  
Uv Crosslinking ◽  
Chlorotic Leaf ◽  
Rna Binding Domain

The RNA-binding properties of the cell-to-cell movement protein (MP) of Apple chlorotic leaf spot virus were analysed. MP was expressed in Escherichia coli and was used in UV-crosslinking analysis, using a digoxigenin–UTP-labelled RNA probe and gel-retardation analysis. The analyses demonstrated that MP bound cooperatively to single-stranded RNA (ssRNA). When analysed for NaCl dependence of the RNA-binding activity, the majority of the MP could bind ssRNA even in binding buffer with 1 M NaCl. Furthermore, competition binding experiments showed that the MP bound preferentially to ssRNA and single-stranded DNA without sequence specificity. MP deletion mutants were used to identify the RNA-binding domain by UV-crosslinking analysis. Amino acid residues 82–126 and 127–287 potentially contain two independently active, single-stranded nucleic acid-binding domains.

Molecular Characterization and Distribution of Apple Chlorotic Leaf Spot Virus on Apple in China

2013 ◽  
Vol 162 (5) ◽  
pp. 284-290
Author(s):  
Hao Duan ◽  
Zhirui Ji ◽  
Shutong Wang ◽  
Tongle Hu ◽  
Yanan Wang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Leaf Spot ◽  
Spot Virus ◽  
Chlorotic Leaf

scholarly journals Survey of Apple Chlorotic Leaf Spot Virus and Apple Stem Grooving Virus Occurrence in Korea and Frequency of Mixed Infections in Apple

2015 ◽  
Vol 60 (2) ◽  
pp. 323-329 ◽  
Author(s):  
Jae-Yeong Han ◽  
Jung-Kyu Kim ◽  
Jin–Soo Cheong ◽  
Eun–Yeong Seo ◽  
Chan–Hwan Park ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Mixed Infections ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf

CHAPTER 17: Apple chlorotic leaf spot virus in Stone Fruits

2011 ◽  
pp. 85-90 ◽  
Author(s):  
A. Myrta ◽  
S. Matic ◽  
T. Malinowski ◽  
G. Pasquini ◽  
T. Candresse
Keyword(s):  
Leaf Spot ◽  
Stone Fruits ◽  
Spot Virus ◽  
Chlorotic Leaf
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