scholarly journals Survey of Apple Chlorotic Leaf Spot Virus and Apple Stem Grooving Virus Occurrence in Korea and Frequency of Mixed Infections in Apple

2015 ◽  
Vol 60 (2) ◽  
pp. 323-329 ◽  
Author(s):  
Jae-Yeong Han ◽  
Jung-Kyu Kim ◽  
Jin–Soo Cheong ◽  
Eun–Yeong Seo ◽  
Chan–Hwan Park ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Mixed Infections ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf

Distribution of apple stem grooving virus and apple chlorotic leaf spot virus in infected in vitro pear shoots

2010 ◽  
Vol 29 (12) ◽  
pp. 1447-1451 ◽  
Author(s):  
L.P. Wang ◽  
N. Hong ◽  
G.P. Wang ◽  
W.X. Xu ◽  
R. Michelutti ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf

scholarly journals Gospodarski važni virusi jezgričavog voća

2019 ◽  
Vol 22 (3-4) ◽  
pp. 123-136
Author(s):  
Dario Ivić
Keyword(s):  
Mosaic Virus ◽  
Leaf Spot ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting

Virusi jabuke, kruške ili dunje relativno su slabo poznati stručnjacima i voćarima. Najvažnijim virusima koji se javljaju na jezgričavim voćnim vrstama smatraju se virus mozaika jabuke (Apple mosaic virus, ApMV), virus klorotične pjegavosti lista jabuke (Apple chlorotic leaf spot virus, ACLSV), virus brazdavosti debla jabuke (Apple stem grooving virus, ASGV) i virus jamičavosti debla jabuke (Apple stem pitting virus,ASPV). U radu je ukratko opisana njihova važnost, biologija i regulativni status, kao i osnovne mjere zaštite.

scholarly journals First Report of Apple Top Working Disease Caused by Viruses (Apple stem grooving virus, Apple chlorotic leaf spot virus, and Apple stem pitting virus) in Apple in India

Plant Disease ◽  
2013 ◽  
Vol 97 (7) ◽  
pp. 1001-1001 ◽  
Author(s):  
A. Brakta ◽  
P. D. Thakur ◽  
A. Handa
Keyword(s):  
Leaf Spot ◽  
Spot Virus ◽  
Sequence Identity ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Commercial Cultivars ◽  
Chlorotic Leaf ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting ◽  
Das Elisa

Top working of apple (Malus domestica Borkh.) trees of old, unproductive, and less preferred cultivars with the newly introduced spur type commercial cultivars has become a common practice with many growers in the northwestern Himalayan region of India. Typical viral symptoms of curling, puckering, and necrosis on leaves were observed with an incidence of 80% on Red Chief, Super Chief, Scarlet Spur, Schillet Spur, Washington Red Delicious, and many other newly introduced cultivars during surveys conducted in May and June 2009. Leaf samples from top worked trees were tested for the presence of Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), and Apple stem pitting virus (ASPV) by employing biological detection (herbaceous and woody indicators), double antibody sandwich (DAS)-ELISA), and reverse transcriptase (RT)-PCR based detection. Mechanical transmission to herbaceous indicators produced chlorotic lesions on Chenopodium quinoa and C. amaranticolor, whereas marginal necrosis was induced on Phaseolous vulgaris within 9 to 21 days after sap inoculations. All three viruses, i.e., ASGV, ASPV, and ACLSV, were detected from these herbaceous indicators in DAS-ELISA (BIOREBA AG, Switzerland). Furthermore, symptoms similar to those observed in orchards were produced when the test budwood was inoculated onto the woody indicator (M. pumila ‘Spy 227’) plant by double grafting, grafting cum budding, and double budding methods within time periods ranging from 4 months in double grafting, 5 months in double budding, to 1 year 4 months in the grafting cum budding method. The presence of all three viruses was confirmed by DAS-ELISA again in Spy 227 woody indicator. PCR detection was carried out by using the coat protein gene specific primers (ASGV5641 [forward], ASGV6396 [reverse]; ACLSV6784 [forward], ACLSV7365 [reverse] [2]; ASP-C [sense], ASP-A [anti-sense] [1]) of all the viruses detected through ELISA. The amplified products were cloned, sequenced, and deposited in NCBI (GenBank Accessions KC110892 for ASGV, KC154859 for ASPV, and KC154862 for ACLSV). BLASTn analysis showed the ASGV isolate had 97 to 98% sequence identity with Indian (FM204881) and Brazilian (AF438409) ASGV isolates. The ASPV and ACLSV isolates had 98% and 99% sequence identity with Chinese (JF895517) and Japanese (AB326230) isolates, respectively. To the best of our knowledge, this is the first report of apple top working disease associated with ASGV, ASPV, and ACLSV infection in commercial cultivars of apple from India and seems to be a serious threat for growing virus-free healthy stocks in orchards. Top working disease in apple associated with ASGV, ASPV, and ACLSV viruses has been reported from Japan (3,4). References: (1) J. K. Kundu et al. Plant Prot. Sci. 39:88, 2003. (2) O. Nickel et al. Fitopatol. Brasil. 26:655, 2001. (3) H. Yanase. Bull. Fruit Tree Res. Stn., Japan Ser. C 1:47, 1974. (4) H. Yanase et al. Acta Hortic. 44:221, 1975.

scholarly journals First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2293
Author(s):  
Celia Canales ◽  
Félix Morán ◽  
Antonio Olmos ◽  
Ana Belén Ruiz-García
Keyword(s):  
Leaf Spot ◽  
High Throughput Sequencing ◽  
Eriobotrya Japonica ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf ◽  
First Time ◽  
Leaf Virus

Loquat (Eriobotrya japonica) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and high-throughput sequencing (HTS) of symptomatic loquat plants led to first-time detection and characterization of apple stem grooving virus (ASGV), also known as citrus tatter leaf virus (CTLV), and apple chlorotic leaf spot virus (ACLSV) from Spain with description of nearly complete genomic sequences. The frequency of ACLSV infection was the highest, with over 30% of the samples testing positive and were also detected as coinfections with ASGV and ASPV, although most of the samples infected were symptomless. Studies on all the full-length sequences available in the databases were performed in order to establish the phylogenetic relationships of the Spanish isolates of these two viral species. Moreover, apple hammerhead viroid (AHVd) was also detected to infect loquat, the first host different from apple reported for this viroid to date.

scholarly journals Loquat Is a New Natural Host of Apple Stem Grooving Virus and Apple Chlorotic Leaf Spot Virus in China

Plant Disease ◽  
2019 ◽  
Vol 103 (12) ◽  
pp. 3290-3290 ◽  
Author(s):  
Qiyan Liu ◽  
Zhiyou Xuan ◽  
Jiaxing Wu ◽  
Yuanjian Qiu ◽  
Min Li ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Natural Host ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf
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