Effects ofβ 2-adrenergic agonists on isolated guinea pig lung mast cells

1994 ◽  
Vol 42 (3-4) ◽  
pp. 92-94 ◽  
Author(s):  
H. Y. A. Lau ◽  
P. L. Wong ◽  
C. K. W. Lai
Keyword(s):  
Mast Cells ◽  
Guinea Pig ◽  
Adrenergic Agonists

Effects of Anaphylactic Shock and Compound 48/80 on the Mast Cells of the Guinea Pig Lung

Nature ◽  
1956 ◽  
Vol 177 (4505) ◽  
pp. 427-429 ◽  
Author(s):  
IVAN MOTA ◽  
ITAMAR VUGMAN
Keyword(s):  
Mast Cells ◽  
Guinea Pig ◽  
Anaphylactic Shock

The histamine content of guinea pig mast cells

1960 ◽  
Vol 16 (5) ◽  
pp. 192-192 ◽  
Author(s):  
L. O. Boréus ◽  
N. Chakravarty
Keyword(s):  
Mast Cells ◽  
Guinea Pig ◽  
Histamine Content

Guinea Pig Mast Cells: Comparative Study on Morphology, Fixation and Staining Properties

1988 ◽  
Vol 85 (3) ◽  
pp. 351-357 ◽  
Author(s):  
N.S. Ghanem ◽  
E.S.K. Assem ◽  
K.B.P. Leung ◽  
F.L. Pearce
Keyword(s):  
Mast Cells ◽  
Guinea Pig ◽  
Comparative Study

scholarly journals Immunocytochemical localization of histamine in secretory granules of rat peritoneal mast cells with conventional or rapid microwave fixation and an ultrastructural post-embedding immunogold technique.

1992 ◽  
Vol 40 (9) ◽  
pp. 1247-1256 ◽  
Author(s):  
G R Login ◽  
S J Galli ◽  
A M Dvorak
Keyword(s):  
Electron Microscopy ◽  
Mast Cells ◽  
Guinea Pig ◽  
Secretory Granules ◽  
Fixation Method ◽  
Structural Localization ◽  
Thin Sections ◽  
Aldehyde Fixation ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

We used a post-embedding immunogold labeling approach to define the fine-structural localization of histamine in rat peritoneal mast cells that were fixed using either standard aldehyde fixation or a fast microwave-aldehyde fixation method. Specimens were processed routinely for electron microscopy. Thin sections were exposed first to guinea pig antihistamine antiserum and then to gold-conjugated goat IgG directed against guinea pig IgG. By transmission electron microscopy, gold particles were localized to the matrix of cytoplasmic granules. Control sections treated with non-immune sera did not show labeling of mast cells. Adsorption of antihistamine antiserum with purified histamine or histamine bound to agarose showed a significant reduction (p less than 0.005) in granule staining. We also confirmed that our isolation procedures yielded functionally competent mast cells which released histamine when stimulated with sheep anti-rat IgE antiserum or with compound 48/80. These studies define the conditions of fixation for electron microscopy that are appropriate for the localization of histamine in the granule matrix of rat peritoneal mast cells.

Isolation and some properties of mast cells from the mesentery of the rat and guinea pig

1980 ◽  
Vol 10 (1-2) ◽  
pp. 124-131 ◽  
Author(s):  
Frederick L. Pearce ◽  
Madeleine Ennis
Keyword(s):  
Mast Cells ◽  
Guinea Pig

scholarly journals DEMONSTRATION OF PEROXIDASE ACTIVITY IN TISSUE SECTIONS

1964 ◽  
Vol 12 (7) ◽  
pp. 538-544 ◽  
Author(s):  
MAX WACHSTEIN ◽  
ELIZABETH MEISEL
Keyword(s):  
Salivary Gland ◽  
Mast Cells ◽  
Guinea Pig ◽  
Peroxidase Activity ◽  
Striated Muscle ◽  
Acinar Cells ◽  
Hair Follicles ◽  
Tissue Sections ◽  
Pig Skin ◽  
Mammalian Tissues

By using an improved benzidine technique, peroxidase activity can be demonstrated in various locations in mammalian tissues. A relatively formalin resistant enzyme is found in hemoglobin and is also associated with mitochondria of striated muscle and heart. A somewhat less formalin resistant peroxidase occurs in the granules of myeloid and mast cells. A relatively formalin sensitive peroxidase is present in a number of additional locations, e.g. the acinar cells in thyroid and salivary gland, the medulla of the kidney, in hair follicles of the guinea pig skin and Kupffer cells of the liver.

scholarly journals Histamine-containing peripheral neuronal and endocrine systems.

1985 ◽  
Vol 33 (9) ◽  
pp. 933-941 ◽  
Author(s):  
P Panula ◽  
M Kaartinen ◽  
M Mäcklin ◽  
E Costa
Keyword(s):  
Mast Cells ◽  
Guinea Pig ◽  
Peripheral Nerves ◽  
Endocrine Cells ◽  
Ganglion Cell Layer ◽  
Cell Layer ◽  
Nerve Fibers ◽  
Immunohistochemical Method ◽  
Rat Stomach ◽  
Rat Ileum

An immunohistochemical method was developed to detect histamine in tissues. The aim of this study was to reveal the cellular stores of histamine in the gastrointestinal tract, pituitary, and adrenal gland. Histamine-containing nerve fibers were found in both rat and guinea pig gut. The origin of at least some of these fibers in the rat ileum was the submucous ganglion cell layer. In the rat stomach, numerous enterochromaffin-like cells exhibited histamine immunofluorescence, and endocrine cells in the ileum and jejunum contained histamine. Only mast cells contained histamine in the neurohypophysis. A large number of process-bearing cells in the guinea pig but not in the rat adrenal medulla contained histamine. The study shows that histamine is present in peripheral nerves and endocrine cells in addition to mast cells, and may function as a neurotransmitter or hormone.

Studies on segmental differences in sensitivity to adrenergic agonists in pulmonary arteries isolated from the guinea-pig

1982 ◽  
Vol 82 (3-4) ◽  
pp. 137-146 ◽  
Author(s):  
Carl K. Buckner ◽  
Sergio Cueva ◽  
Shtaywy Abdalla ◽  
James A. Will
Keyword(s):  
Guinea Pig ◽  
Pulmonary Arteries ◽  
Adrenergic Agonists
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