Guinea Pig Mast Cells: Comparative Study on Morphology, Fixation and Staining Properties

1988 ◽  
Vol 85 (3) ◽  
pp. 351-357 ◽  
Author(s):  
N.S. Ghanem ◽  
E.S.K. Assem ◽  
K.B.P. Leung ◽  
F.L. Pearce
Keyword(s):  
Mast Cells ◽  
Guinea Pig ◽  
Comparative Study

Comparative Study of Proactivators of the Fibrinolysin System in Three Mammalian Species

1969 ◽  
Vol 21 (03) ◽  
pp. 594-603 ◽  
Author(s):  
Y Takada ◽  
A Takada ◽  
J. L Ambrus
Keyword(s):  
Guinea Pig ◽  
Comparative Study ◽  
Human Plasma ◽  
Mammalian Species ◽  
Gel Filtration ◽  
Plasminogen Activators ◽  
The Other ◽  
Rabbit Plasma ◽  
Plate Test ◽  
Fibrin Plate

SummarySephadex gel filtration of human plasma gave results suggesting the presence of two proactivators of plasminogen, termed proactivators A and B.Activity resembling that of proactivator A was found in rabbit plasma, but not in guinea pig plasma.Plasminogen activators produced by the interaction of proactivator A of human plasma with streptokinase had no caseinolytic or TAMe esterolytic effect.Proactivator A can be separated in a form apparently free from plasminogen, as shown by the heated fibrin plate test and by immunological analysis. On the other hand, proactivator B concentrates prepared so far are contamined with plasminogen.Human proactivators appear to be far more susceptible to streptokinase than are rabbit proactivators.Inhibitors of the fibrinolysin system were observed in the plasmas of all 3 species. These inhibitors are not present in the euglobulin fraction of plasma. Sephadex fractionation of euglobulin fractions results in proactivator preparations that do not contain inhibitors.

Effects of Anaphylactic Shock and Compound 48/80 on the Mast Cells of the Guinea Pig Lung

Nature ◽  
1956 ◽  
Vol 177 (4505) ◽  
pp. 427-429 ◽  
Author(s):  
IVAN MOTA ◽  
ITAMAR VUGMAN
Keyword(s):  
Mast Cells ◽  
Guinea Pig ◽  
Anaphylactic Shock

The histamine content of guinea pig mast cells

1960 ◽  
Vol 16 (5) ◽  
pp. 192-192 ◽  
Author(s):  
L. O. Boréus ◽  
N. Chakravarty
Keyword(s):  
Mast Cells ◽  
Guinea Pig ◽  
Histamine Content

Comparative study of ozone (O3) uptake in three strains of rats and in the guinea pig

1988 ◽  
Vol 96 (1) ◽  
pp. 140-146 ◽  
Author(s):  
Mildred J. Wiester ◽  
Jeffrey S. Tepper ◽  
Mitchell E. King ◽  
Margaret G. Ménache ◽  
Daniel L. Costa
Keyword(s):  
Guinea Pig ◽  
Comparative Study

scholarly journals Immunocytochemical localization of histamine in secretory granules of rat peritoneal mast cells with conventional or rapid microwave fixation and an ultrastructural post-embedding immunogold technique.

1992 ◽  
Vol 40 (9) ◽  
pp. 1247-1256 ◽  
Author(s):  
G R Login ◽  
S J Galli ◽  
A M Dvorak
Keyword(s):  
Electron Microscopy ◽  
Mast Cells ◽  
Guinea Pig ◽  
Secretory Granules ◽  
Fixation Method ◽  
Structural Localization ◽  
Thin Sections ◽  
Aldehyde Fixation ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

We used a post-embedding immunogold labeling approach to define the fine-structural localization of histamine in rat peritoneal mast cells that were fixed using either standard aldehyde fixation or a fast microwave-aldehyde fixation method. Specimens were processed routinely for electron microscopy. Thin sections were exposed first to guinea pig antihistamine antiserum and then to gold-conjugated goat IgG directed against guinea pig IgG. By transmission electron microscopy, gold particles were localized to the matrix of cytoplasmic granules. Control sections treated with non-immune sera did not show labeling of mast cells. Adsorption of antihistamine antiserum with purified histamine or histamine bound to agarose showed a significant reduction (p less than 0.005) in granule staining. We also confirmed that our isolation procedures yielded functionally competent mast cells which released histamine when stimulated with sheep anti-rat IgE antiserum or with compound 48/80. These studies define the conditions of fixation for electron microscopy that are appropriate for the localization of histamine in the granule matrix of rat peritoneal mast cells.

Isolation and some properties of mast cells from the mesentery of the rat and guinea pig

1980 ◽  
Vol 10 (1-2) ◽  
pp. 124-131 ◽  
Author(s):  
Frederick L. Pearce ◽  
Madeleine Ennis
Keyword(s):  
Mast Cells ◽  
Guinea Pig
Sign in / Sign up

Export Citation Format

Share Document