Kinetic analysis of the inhibition of sulfate transport in human red blood cells by isothiocyanates

1978 ◽  
Vol 41 (2) ◽  
pp. 101-115 ◽  
Author(s):  
Emmanuel T. Rakitzis ◽  
Paul J. Gilligan ◽  
Joseph F. Hoffman
Keyword(s):  
Red Blood Cells ◽  
Kinetic Analysis ◽  
Blood Cells ◽  
Sulfate Transport ◽  
Human Red Blood Cells

scholarly journals Influence of gemfibrozil on sulfate transport in human erythrocytes during the oxygenation-deoxygenation cycle

2008 ◽  
pp. 621-629
Author(s):  
E Tellone ◽  
S Ficarra ◽  
R Scatena ◽  
B Giardina ◽  
A Kotyk ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Significant Role ◽  
Blood Cells ◽  
Band 3 ◽  
Macromolecular Complex ◽  
Sulfate Transport ◽  
Human Red Blood Cells ◽  
Anion Transporter ◽  
Chicken Erythrocytes ◽  
T State

The effects of gemfibrozil (GFZ), an antihyperlipidemic agent, on the anionic transport of the human red blood cells (RBC) during the oxygenation-deoxygenation cycle were examined. Gemfibrozil clearly plays a role in the modulation of the anionic flux in erythrocytes; in fact it causes a strong increment of anions transport when the RBCs are in the high-oxygenation state (HOS). Such an effect is remarkably reduced in the lowoxygenation state (LOS). With the aim of identifying the dynamics of fibrate action, this effect has been investigated also in human ghost and chicken erythrocytes. These latter, in fact, are known to possess a B3 (anion transporter or Band 3) modified at the cytoplasmic domain (cdb3) which plays a significant role in the metabolic modulation of red blood cells. The results were analyzed taking into account the well-known interactions between fibrates and both conformational states of hemoglobin i.e. the T state (deoxy-conformation) and the R state (oxy-conformation). The effect of gemfibrozil on anionic influx appears to be due to a wide interaction involving a “multimeric” Hb-GFZ-cdb3 macromolecular complex.

scholarly journals A kinetic analysis of Na-Li countertransport in human red blood cells.

1986 ◽  
Vol 87 (3) ◽  
pp. 353-368 ◽  
Author(s):  
P A Hannaert ◽  
R P Garay
Keyword(s):  
Red Blood Cells ◽  
Kinetic Analysis ◽  
Blood Cells ◽  
Kinetic Properties ◽  
Maximal Rate ◽  
Apparent Dissociation Constant ◽  
Human Red Blood Cells ◽  
Ping Pong ◽  
Na Efflux ◽  
Cation Sites

We examined the kinetic properties of the interactions between inner and outer cation sites of the Na-Li countertransport system in human red blood cells. Li-stimulated Na efflux [V(Na)] was measured as a function of external Li [(Li)o] and internal Na [(Na)i] contents. At each (Li)o, a Hanes plot of (Na)i/V(Na) vs. (Na)i allowed us to calculate the apparent dissociation constant for internal Na (KiNa) and the maximal rate of Na efflux [Vmax(Na)]. In erythrocytes from 10 different subjects, the Vmax(Na)/KiNa ratios were independent of the external Li concentrations. In other experiments, Na-stimulated Li efflux [V(Li)] was measured as a function of external Na and internal Li contents. In three subjects studied, the Vmax(Li)/KiLi ratios were independent of the external Na concentrations. The data strongly suggest that the countertransport mechanism is consecutive ("ping-pong").

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