l-leucine transport in human red blood cells: A detailed kinetic analysis

1981 ◽  
Vol 62 (1-2) ◽  
pp. 79-93 ◽  
Author(s):  
Raben Rosenberg
Keyword(s):  
Red Blood Cells ◽  
Kinetic Analysis ◽  
Blood Cells ◽  
Human Red Blood Cells ◽  
Leucine Transport

Kinetic analysis of the inhibition of sulfate transport in human red blood cells by isothiocyanates

1978 ◽  
Vol 41 (2) ◽  
pp. 101-115 ◽  
Author(s):  
Emmanuel T. Rakitzis ◽  
Paul J. Gilligan ◽  
Joseph F. Hoffman
Keyword(s):  
Red Blood Cells ◽  
Kinetic Analysis ◽  
Blood Cells ◽  
Sulfate Transport ◽  
Human Red Blood Cells

scholarly journals A kinetic analysis of Na-Li countertransport in human red blood cells.

1986 ◽  
Vol 87 (3) ◽  
pp. 353-368 ◽  
Author(s):  
P A Hannaert ◽  
R P Garay
Keyword(s):  
Red Blood Cells ◽  
Kinetic Analysis ◽  
Blood Cells ◽  
Kinetic Properties ◽  
Maximal Rate ◽  
Apparent Dissociation Constant ◽  
Human Red Blood Cells ◽  
Ping Pong ◽  
Na Efflux ◽  
Cation Sites

We examined the kinetic properties of the interactions between inner and outer cation sites of the Na-Li countertransport system in human red blood cells. Li-stimulated Na efflux [V(Na)] was measured as a function of external Li [(Li)o] and internal Na [(Na)i] contents. At each (Li)o, a Hanes plot of (Na)i/V(Na) vs. (Na)i allowed us to calculate the apparent dissociation constant for internal Na (KiNa) and the maximal rate of Na efflux [Vmax(Na)]. In erythrocytes from 10 different subjects, the Vmax(Na)/KiNa ratios were independent of the external Li concentrations. In other experiments, Na-stimulated Li efflux [V(Li)] was measured as a function of external Na and internal Li contents. In three subjects studied, the Vmax(Li)/KiLi ratios were independent of the external Na concentrations. The data strongly suggest that the countertransport mechanism is consecutive ("ping-pong").

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