Early kinetics of Ca2+ fluxes and histamine release in rat mast cells stimulated with compound 48/80

1984 ◽  
Vol 15 (3-4) ◽  
pp. 279-284 ◽  
Author(s):  
David E. McClain ◽  
Mildred A. Donlon ◽  
Thomas A. Hill ◽  
George N. Catravas
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Kinetics Of ◽  
Rat Mast Cells

Extracellular effect of calcium on compound 48/80 stimulated mast cells

1983 ◽  
Vol 61 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Narendranath S. Ranadive ◽  
Rubina Lewis
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
High Concentration ◽  
Extracellular Concentration ◽  
Exchangeable Ca ◽  
Intracellular Ca ◽  
Kinetics Of ◽  
Rat Mast Cells

The effects of changes in the extracellular concentration of calcium on activation of rat mast cells by compound 48/80 were studied. The intracellular exchangeable Ca2+ pools at various concentrations of extracellular Ca2+ were determined by equilibration of the cells with 45Ca2+. The cells stimulated by compound 48/80 in the presence of 2.5 μM and 1.6 mM extracellular Ca2+ released comparable amounts of histamine. However, the intracellular Ca2+ pool was doubled in 2.5 μM Ca2+ and was increased sixfold in 1.6 mM Ca2+. In 14.4 mM extracellular Ca2+, there was neither release of histamine nor uptake of Ca2+ which suggested an impairment in activation. The kinetics of Ca2+ influx in the presence of 2.5 μM Ca2+ did not reveal intracellular mobilization of calcium. The cells activated in 1.6 mM Ca2+ at 0 °C when allowed to stand in 14.4 mM extracellular Ca2+ released decreased amounts of histamine upon warming to 37 °C. The inhibition of the release progressively increased with time of standing at 0 °C. The decrease in histamine release was not seen with the cells standing in 1.6 mM Ca2+ at 0 °C. The effect of 14.4 mM Ca2+ added prior to the challenge with compound 48/80 did not depend on the time of incubation. The data presented in this paper suggest that the high concentration of Ca2+ inhibits the histamine release from mast cells by interfering with membrane-associated phenomena.

83. Histamine release from rat mast cells by antibodies against IgE-receptors

1978 ◽  
Vol 61 (3) ◽  
pp. 153 ◽  
Author(s):  
T ISHIZAKA ◽  
K ISHIZAKA ◽  
D CONRAD ◽  
A FROESE
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Ige Receptors ◽  
Rat Mast Cells

scholarly journals Inhibitory effects of stilbenes on histamine release from rat mast cells

1984 ◽  
Vol 36 ◽  
pp. 232
Author(s):  
Makoto Matsumoto ◽  
Tamiko Suzuki ◽  
Masaatsu K. Uchida
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Inhibitory Effects ◽  
Rat Mast Cells

Differential Effects of Sodium Cromoglycate on Adriamycin-Induced Histamine Release from Isolated Rat and Mouse Mast Cells

1988 ◽  
Vol 1 (2) ◽  
pp. 115-118
Author(s):  
Giuliana Decorti ◽  
Fiora Bartoli Klugmann ◽  
L. Candussio ◽  
L. Baldini
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Sodium Cromoglycate ◽  
Inhibitory Activity ◽  
Antineoplastic Drug ◽  
Differential Effects ◽  
Isolated Rat ◽  
Rat Mast Cells

Adriamycin-induced histamine release has been studied on purified mast cells obtained from peritoneal cavities of mice and rats. The activity of the antineoplastic drug was compared with that of the classic basic secretagogue compound 48/80. Rat and mouse mast cells were similarly responsive to adriamycin, on the contrary, the activity of compound 48/80 was more marked in rat than in mouse. The antianaphylactic agent sodium cromoglycate was extremely effective in preventing adriamycin-induced histamine release in both species, whereas, an inhibitory activity against compound 48/80 was evident only in rat mast cells.

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