The effects of fractions (Chalones) obtained from lymphoid organs on lymphocyte proliferation in vitro

1977 ◽  
Vol 7 (3) ◽  
pp. 327-335 ◽  
Author(s):  
P. C. Hiestand ◽  
J. F. Borel ◽  
W. Bauer ◽  
Z. L. Kis ◽  
C. Magnée ◽  
...  
Keyword(s):  
Lymphocyte Proliferation ◽  
Lymphoid Organs ◽  
Proliferation In Vitro

Subset-Specific Effects of Sex Hormones and Pituitary Gonadotropins on Human Lymphocyte Proliferation in Vitro

1993 ◽  
Vol 66 (3) ◽  
pp. 201-211 ◽  
Author(s):  
Balu H. Athreya ◽  
Jonathan Pletcher ◽  
Francesco Zulian ◽  
David B. Weiner ◽  
William V. Williams
Keyword(s):  
Sex Hormones ◽  
Human Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Specific Effects ◽  
Proliferation In Vitro

Effects of Type and Amount of Dietary Fat on Rabbit and Rat Lymphocyte Proliferation In Vitro

1988 ◽  
Vol 118 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Emile A. M. De Deckere ◽  
Christina Jansen Verplanke ◽  
Cornelis G. Blonk ◽  
Wilhelmus G. L. Van Nielen
Keyword(s):  
Dietary Fat ◽  
Lymphocyte Proliferation ◽  
Proliferation In Vitro

scholarly journals Lymphocyte transformation induced by autologous cells. V. generation of immunologic memory and specificity during the autologous mixed lymphocyte reaction

1977 ◽  
Vol 146 (6) ◽  
pp. 1833-1838 ◽  
Author(s):  
ME Weksler ◽  
R Kozak
Keyword(s):  
T Lymphocytes ◽  
Lymphocyte Proliferation ◽  
Mixed Lymphocyte Reaction ◽  
Protein A ◽  
Lymphocyte Transformation ◽  
Cell Mediated Immunity ◽  
Staphylococcal Protein A ◽  
Autologous Mixed Lymphocyte Reaction ◽  
Proliferation In Vitro

Lymphocyte proliferation in vitro may follow antigen recognition and serve as a correlate of cell-mediated immunity. Lymphocyte proliferation can also be simulated by nonimmune mechanisms as, for example, following culture with plant lectin, lipopolysaccharides, or staphylococcal protein A (1). The autologous mixed lymphocyte reaction (MLR) refers to the proliferation of T lymphocytes cultured with autologous mon-T lymphocytes (2,3). The purpose of this study was to determine whether lymphocyte proliferation in the autologous MLR results from immune or nonimmune mechanisms. We have shown that the autologous MLR has two classical attributes of an immune phenomenon: memory and specificity.

scholarly journals LASER MODULATION OF T-LYMPHOCYTE PROLIFERATION IN VITRO

LASER THERAPY ◽  
1998 ◽  
Vol 10 (4) ◽  
pp. 153-158 ◽  
Author(s):  
Atif Agaiby ◽  
Lucy Ghali ◽  
Mary Dyson
Keyword(s):  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Laser Modulation ◽  
T Lymphocyte Proliferation ◽  
Proliferation In Vitro

Ganciclovir Suppresses Human T Lymphocyte Proliferation In Vitro.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 5378-5378
Author(s):  
Minoo Battiwalla ◽  
Yiyuan Wu ◽  
Ryotaro Nakamura ◽  
Marija Radovic ◽  
Rajinder P.S. Bajwa ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Brdu Incorporation ◽  
Human Pbmcs ◽  
Post Transplant ◽  
T Lymphocyte Proliferation ◽  
Proliferation In Vitro ◽  
Cmv Reactivation

Abstract Clinically significant cytomegalovirus (CMV) infection in allogeneic blood or marrow transplant recipients has dramatically declined in recent years by the strategy of early detection of reactivation and pre-emptive therapy with Ganciclovir (GCV). We have previously shown that even in the absence of overt CMV disease, persisting post-transplant antigenemia predicts for increased late relapse and treatment failure. (Nakamura, et al BBMT 2004) In other words, frequent CMV reactivation serves as a surrogate for impaired post-transplant immune reconstitution. To explain the observed association between CMV reactivation and relapse we also raised the possibility that several weeks of GCV therapy could exert a deleterious effect on a fragile immune system. Clinical association between GCV administration and impaired lymphocyte function has not received attention previously; perhaps because of confounding effects from the underlying conditions (HIV or post-transplant) that induce CMV reactivation. We examined the effect of GCV in vitro on normal human PBMCs. Human PBMCs were extracted from normal volunteers and subjected to mitogenic stimulation (PHA) in the absence or presence of varying concentrations of GCV. PHA-induced proliferation, measured by uptake of 3[H]-thymidine after 5 days incubation in RPMI-10% AB serum, was reduced by 35% at peak therapeutic concentrations (10mg/ml) of GCV as opposed to 73% by Tacrolimus (10ng/ml). GCV did not induce lymphocyte apoptosis in the presence or absence of stimulation. Flow cytometry-based BrdU incorporation assays show that GCV exerts a time-dependent impairment of DNA synthesis in lymphocytes. Collectively, these results show that GCV suppresses T-lymphocyte proliferation in vitro at therapeutic concentrations and the likely mechanism of action is inhibition of DNA synthesis. Further work is ongoing to evaluate the effect of GCV on proliferative responses to specific antigens and to confirm these effects in comparison to other drugs used in the transplant setting. Figure Figure

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