Cellular localization of androgen in the brain and pituitary after the injection of tritiated testosterone

1972 ◽  
Vol 28 (11) ◽  
pp. 1364-1366 ◽  
Author(s):  
Madhabananda Sar ◽  
W. E. Stumpf
Keyword(s):  
Cellular Localization ◽  
The Brain

scholarly journals Cyclooxygenase in the brain: their cellular localization and functions

Ensho Saisei ◽  
2001 ◽  
Vol 21 (3) ◽  
pp. 209-217
Author(s):  
Kiyoshi Matsumura ◽  
Shigeo Kobayashi
Keyword(s):  
Cellular Localization ◽  
The Brain

scholarly journals Synthesis of transthyretin (pre-albumin) mRNA in choroid plexus epithelial cells, localized by in situ hybridization in rat brain.

1986 ◽  
Vol 34 (7) ◽  
pp. 949-952 ◽  
Author(s):  
A J Stauder ◽  
P W Dickson ◽  
A R Aldred ◽  
G Schreiber ◽  
F A Mendelsohn ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Epithelial Cells ◽  
Rat Brain ◽  
Choroid Plexus ◽  
Cellular Localization ◽  
Lateral Ventricles ◽  
The Brain ◽  
Albumin Mrna ◽  
Choroid Plexus Epithelial

The sites of synthesis of transthyretin in the brain were investigated using in situ hybridization with [35S]-labeled recombinant cDNA probes specific for transthyretin mRNA. Autoradiography of hybridized coronal sections of rat brain revealed specific cellular localization of transthyretin mRNA in choroid plexus epithelial cells of the lateral, third, and fourth ventricles. Transferrin mRNA was also investigated and, in contrast to transthyretin mRNA, was localized mainly in the lateral ventricles. Our results indicate that substantial synthesis of transthyretin and transferrin mRNA may occur in the choroid plexus.

CELLULAR LOCALIZATION OF A PROLACTIN-LIKE ANTIGEN IN THE RAT BRAIN

1979 ◽  
Vol 83 (2) ◽  
pp. 261-NP ◽  
Author(s):  
G. TOUBEAU ◽  
J. DESCLIN ◽  
M. PARMENTIER ◽  
J. L. PASTEELS
Keyword(s):  
Rat Brain ◽  
Cellular Localization ◽  
Female Rats ◽  
Nerve Fibres ◽  
Male And Female ◽  
Immunoreactive Material ◽  
Paraventricular Nuclei ◽  
Male And Female Rats ◽  
The Brain

The distribution of immunoreactive neurones and fibres was studied in rat brain using an antiserum to rat prolactin. Neurones containing the immunoreactive material were localized in the arcuate, ventromedial, premamillary, supraoptic and paraventricular nuclei of the hypothalamus. Immunoreactive nerve fibres were widely distributed within the brain. No differences were observed in labelling between male and female rats, or as a consequence of hypophysectomy.

Prospect of a stanniocalcin endocrine/paracrine system in mammals

2002 ◽  
Vol 282 (3) ◽  
pp. F367-F375 ◽  
Author(s):  
Kenichi Ishibashi ◽  
Masashi Imai
Keyword(s):  
Cellular Localization ◽  
Mineral Metabolism ◽  
Early Stage ◽  
Gene Encoding ◽  
Corpuscles Of Stannius ◽  
Pathological Conditions ◽  
High Amino Acid ◽  
Pregnancy And Lactation ◽  
Gene Structure And Expression ◽  
The Brain

Stanniocalcin (STC) is a calcium- and phosphate-regulating hormone produced in bony fish by the corpuscles of Stannius, which are located close to the kidney. It is a major antihypercalcemic hormone in fish. As the corpuscles of Stannius are absent, and antihypercalcemic hormones are basically not necessary, in mammals, the discovery of a mammalian homolog, STC1, was surprising and intriguing. STC1 displays a relatively high amino acid sequence identity (∼50%) with fish STC. In contrast to fish STC, STC1 is expressed in many tissues, including kidney. More recently, a human gene encoding the second stanniocalcin-like protein, STC2, was identified. STC2 has a lower identity (∼35%) with STC1 and fish STC. Similar to STC1, STC2 is also expressed in a variety of tissues. Research into the functions of STCs in mammals is still at an early stage, and the ultimate physiological and pathological roles of STCs have not yet been established. A few studies indicate that STC1, similar to fish STC, stimulates phosphate absorption in the kidney and intestine, but the function of STC2 is still unknown. However, several interesting findings have been reported on their cellular localization, gene structure, and expression in different physiological and pathological conditions, which will be clues in elucidating the functions of STCs in mammals. STC1 expression is enhanced by hypertonicity in a kidney cell line or by ischemic injuries and neural differentiation in the brain. STC1 expression in the ovary is also enhanced during pregnancy and lactation. Calcitriol upregulates STC1 and downregulates STC2 expression in the kidney. Interestingly, STC1 and STC2 are expressed in many tumor cell lines, and the expression of STC2 is enhanced by estradiol in breast cancer cells. STC2 is also expressed in pancreatic islets. These results suggest that the biological repertoires of STCs in mammals will be considerably larger than in fish and may not be limited to mineral metabolism. This brief review describes recent progress in mammalian STC research.

Expression (Exp) of FIP200 and Rb in breast cancer (BreastCA) metastasis (met) to the brain.

2011 ◽  
Vol 29 (27_suppl) ◽  
pp. 222-222
Author(s):  
N. Hashemi Sadraei ◽  
M. Burgett ◽  
M. S. Ahluwalia ◽  
R. Tipps ◽  
D. Khosla ◽  
...  
Keyword(s):  
Cellular Localization ◽  
Dna Analysis ◽  
Gene Mutations ◽  
Genetic Alterations ◽  
Copy Number Variations ◽  
Deletion Mutations ◽  
The Us ◽  
Her 2 ◽  
The Brain ◽  
Gene Alterations

222 Background: BreastCA is the 2nd leading cause of cancer deaths in women in the US. Although the outcome for BreastCA with brain met remains poor, there is significant variation in overall survival (OS). Yet, there are no markers or models to predict OS. FIP200 regulates several pathways. In the nucleus, it inhibits cell proliferation by promoting Rb-1 and p21, and in the cytoplasm it promotes cell survival by inhibiting Pyk2 and regulating autophagy. It has been reported that FIP200 gene is deleted or mutated in 20% of primary BreastCAs, and its Exp is correlated with Rb1 levels. FIP200 Exp, its cellular localization, and gene alterations have not been examined in brain met of any cancer. Methods: Brain met tissues of 21 BreastCA patients (pts) biopsied in our institution between 8/2000 and 3/2010 were obtained and Exp of FIP200 and Rb1 were analyzed. Biopsies were selected based on tissue adequacy and prior diagnosis (Dx) of invasive intra-ductal BreastCA. Immunohistochemistry (IHC) was used to evaluate the localization and Exp levels of FIP200 and Rb1 which in turn were tested as predictors of OS. Genetic alterations in FIP200 and Rb1 were examined after DNA extraction. Results: Median time from Dx to met was 23 (range 0-245) months (mos), and median OS from Dx was 43 (range 6-264) mos. Nuclear Exp of Rb1 in < 30% vs. ≥ 30% of cells were seen in 11 vs. 10 pts. Median OS was 39 (range 6-264) mos for Rb1 Exp < 30% and 47 (range 20-190) mo for Rb1 Exp ≥ 30%. Nuclear Exp of FIP200 in <20% vs. ≥ 20% of cells were seen in 13 vs. 8 pts. Median OS was 39 (range 6-264) mo for FIP200 nuclear Exp <20% and 45 (range 43-122) mo for FIP200 Exp ≥ 20%. DNA analysis of 11 pts for copy number variations and loss of heterozygocity (LOH) showed no deletion mutations in FIP200 or Rb1, loss of p53 in 4 of 11, ErbB2 (Her-2) amplification in 4 of 11, and no EGFR gene mutations. Conclusions: The nuclear Exp of Rb1 in brain met from invasive intra-ductal BreastCA may be linked OS. An expanded study is underway to define whether nuclear FIP200 in >20% of cells correlates with nuclear Rb and OS in BreastCA.

Differential Distribution of Four Hyperpolarization-Activated Cation Channels in Mouse Brain

1999 ◽  
Vol 380 (7-8) ◽  
pp. 975-980 ◽  
Author(s):  
S. Moosmang ◽  
M. Biel ◽  
F. Hofmann ◽  
A. Ludwig
Keyword(s):  
Olfactory Bulb ◽  
Mouse Brain ◽  
Cellular Localization ◽  
Pyramidal Neurons ◽  
Cation Channels ◽  
Hcn Channel ◽  
Differential Distribution ◽  
Hippocampal Pyramidal Neurons ◽  
Low Levels ◽  
The Brain

Abstract Hyperpolarization-activated cation currents, termed Ih, are observed in a variety of neurons. Four members of a gene family encoding hyperpolarization-activated cyclic-nucleotide-gated cation channels (HCN1-4) have been cloned. The regional expression and cellular localization of the four HCN channel types in mouse brain was investigated using in situ hybridization. The expression of HCN1 was restricted to the olfactory bulb, cerebral cortex, hippocampus, superior colliculus and cerebellum. In contrast, HCN2 transcripts were found at high levels nearly ubiquitiously in the brain, and the strongest signals were seen in the olfactory bulb, hippocampus, thalamus and brain stem. HCN3 was uniformly expressed at very low levels throughout the brain. Finally, HCN4 transcripts were prominently expressed selectively in the thalamus and olfactory bulb. Some neurons expressed two or more HCN channel transcripts including hippocampal pyramidal neurons (HCN1, HCN2 and low levels of HCN 4) and thalamic relay neurons (HCN2 and HCN4). Our results demonstrate that each HCN channel transcript has a unique distribution in the brain. Furthermore, they suggest that the heterogeneity of neuronal Ih may be, at least in part, due to the differential expression of HCN channel genes.

Studies on the cellular localization of biochemical responses to catecholamines in the brain

1992 ◽  
Vol 29 (3-4) ◽  
pp. 285-288 ◽  
Author(s):  
Eric A. Stone ◽  
Safy M. John ◽  
Guoying Bing ◽  
Zhang Yi
Keyword(s):  
Cellular Localization ◽  
Biochemical Responses ◽  
The Brain

Cellular localization of the brain specificα2-glycoprotein in rat cerebellum: An immunohistological study

Neuroscience ◽  
1982 ◽  
Vol 7 (1) ◽  
pp. 231-237 ◽  
Author(s):  
M.S. Ghandour ◽  
O.K. Langley ◽  
G. Gombos ◽  
G. Vincendon ◽  
K. Warecka
Keyword(s):  
Cellular Localization ◽  
Rat Cerebellum ◽  
Immunohistological Study ◽  
The Brain
Sign in / Sign up

Export Citation Format

Share Document