Methods for isolation ofBordetella pertussis from patients with whooping cough

1988 ◽  
Vol 7 (5) ◽  
pp. 616-620 ◽  
Author(s):  
J. E. Hoppe
Keyword(s):  
Whooping Cough ◽  
Ofbordetella Pertussis

scholarly journals Validation and Implementation of a Diagnostic Algorithm for DNA Detection ofBordetella pertussis,B. parapertussis, andB. holmesiiin a Pediatric Referral Hospital in Barcelona, Spain

2018 ◽  
Vol 57 (1) ◽  
Author(s):  
Ana Valero-Rello ◽  
Desiree Henares ◽  
Lesly Acosta ◽  
Mireia Jane ◽  
Iolanda Jordan ◽  
...  
Keyword(s):  
Internal Control ◽  
Whooping Cough ◽  
Diagnostic Algorithm ◽  
Clinical Samples ◽  
Analytical Validation ◽  
Content Type ◽  
Detection And Identification ◽  
Ofbordetella Pertussis ◽  
Lower Limits

ABSTRACTThis study aimed to validate a comprehensive diagnostic protocol based on real-time PCR for the rapid detection and identification ofBordetella pertussis,Bordetella parapertussis, andBordetella holmesii, as well as its implementation in the diagnostic routine of a reference children’s hospital. The new algorithm included a triplex quantitative PCR (qPCR) targeting IS481gene (inB. pertussis,B. holmesii, and someBordetella bronchisepticastrains), pIS1001(B. parapertussis-specific) andrnaseP as the human internal control. Two confirmatory singleplex tests forB. pertussis(ptxA-Pr) andB. holmesii(hIS1001) were performed if IS481was positive. Analytical validation included determination of linear range, linearity, efficiency, precision, sensitivity, and a reference panel with clinical samples. Once validated, the new algorithm was prospectively implemented in children with clinical suspicion of whooping cough presenting to Hospital Sant Joan de Deu (Barcelona, Spain) over 12 months. Lower limits of detection obtained were 4.4, 13.9, and 27.3 genomic equivalents/ml of sample for IS481(onB. pertussis), pIS1001and hIS1001, and 777.9 forptxA-Pr. qPCR efficiencies ranged from 86.0% to 96.9%. Intra- and interassay variabilities were <3% and <5%, respectively. Among 566 samples analyzed,B. pertussis,B. holmesii, andB. parapertussiswere detected in 11.1%, 0.9% (only in females >4 years old), and 0.2% of samples, respectively. The new algorithm proved to be a useful microbiological diagnostic tool for whooping cough, demonstrating a low rate of other non-pertussisBordetellaspecies in our surveilled area.

Epidemiology of whooping cough & typing ofBordetella pertussis

2013 ◽  
Vol 8 (11) ◽  
pp. 1391-1403 ◽  
Author(s):  
Nicolas Hegerle ◽  
Nicole Guiso
Keyword(s):  
Whooping Cough ◽  
Ofbordetella Pertussis

scholarly journals Characterization of the bvgR Locus ofBordetella pertussis

1998 ◽  
Vol 180 (7) ◽  
pp. 1682-1690 ◽  
Author(s):  
Tod J. Merkel ◽  
Cassia Barros ◽  
Scott Stibitz
Keyword(s):  
Virulence Factors ◽  
Whooping Cough ◽  
Transmembrane Protein ◽  
Gene Encoding ◽  
Environmental Signals ◽  
Reading Frame ◽  
Regulated Expression ◽  
Ofbordetella Pertussis ◽  
Transcriptional Fusions

ABSTRACT Bordetella pertussis, the causative agent of whooping cough, produces a wide array of factors that are associated with its ability to cause disease. The expression and regulation of these virulence factors is dependent upon the bvg locus (originally designated the vir locus), which encodes two proteins: BvgA, a 23-kDa cytoplasmic protein, and BvgS, a 135-kDa transmembrane protein. It is proposed that BvgS responds to environmental signals and interacts with BvgA, a transcriptional regulator which upon modification by BvgS binds to specific promoters and activates transcription. An additional class of genes is repressed by the bvg locus. Expression of this class, thebvg-repressed genes (vrgs [for vir-repressed genes]), is reduced under conditions in which expression of the aforementioned bvg-activated virulence factors is maximal; this repression is dependent upon the presence of an intactbvgAS locus. We have previously identified a locus required for regulation of all of the known bvg-repressed genes inB. pertussis. This locus, designated bvgR, maps to a location immediately downstream of bvgAS. We have undertaken deletion and complementation studies, as well as sequence analysis, in order to identify the bvgR open reading frame and identify the cis-acting sequences required for regulated expression of bvgR. Studies utilizing transcriptional fusions of bvgR to the gene encoding alkaline phosphatase have demonstrated that bvgR is activated at the level of transcription and that this activation is dependent upon an intact bvgAS locus.

WHOOPING-COUGH NOTIFICATIONS

The Lancet ◽  
1980 ◽  
Vol 315 (8167) ◽  
pp. 718
Author(s):  
E Miller
Keyword(s):  
Whooping Cough

CLINICAL AND LABORATORY PECULIARITIES OF WHOOPING COUGH IN CHILDREN OF DIFFERENT AGE GROUPS

2020 ◽  
Vol 99 (6) ◽  
pp. 98-104
Author(s):  
I.V. Babachenko ◽  
◽  
Y.V. Nesterova ◽  
N.V. Skripchenko ◽  
◽  
...  
Keyword(s):  
Whooping Cough ◽  
Severe Disease ◽  
Respiratory Rhythm ◽  
Age Groups ◽  
Low Frequency ◽  
Pertussis Infection ◽  
Group 2 ◽  
Sick Children ◽  
Hematological Changes ◽  
Group 1

Objective of the research: to present the clinical and laboratory peculiarities of modern whooping cough in hospitalized children of different ages. Materials and methods: сlinical and laboratory characteristics of whooping cough were analyzed in 88 hospitalized sick children aged 1 month to 18 years in groups of children: group 1 – children under 1 year old; group 2 – children 1–6 years old; group 3 – children 7–17 years old. DNA of causative agents of pertussis infection was isolated by PCR in nasopharyngeal swabs using a commercial kit AmpliSens®Bordetella multi-FL (Moscow). Results: children of group 1 in 90% (n=43) of cases were not vaccinated against whooping cough, severe forms were recorded in 17% (n=8) of children of the 1st year of life, and in 15% (n=7) – due to respiratory rhythm disturbances. The diagnosis was confirmed by PCR in 94% (n=45) of children, leukocytosis with lymphocytosis was detected in 81,5% (n=101). Along with hematological changes typical for whooping cough, 79% (n=38) of patients in the first year of life had thrombocytosis (>400×109/l), which was most pronounced in severe disease course 511,5 [425; 568,5]×109/l vs 421 [347; 505,5]×109/l; p<0,05, which has no tendency to decrease throughout the entire observation period and correlates with the level of leukocytes (rs=0,69; p<0,001). Patients over 7 years old in 88% (n=21) of cases were vaccinated against whooping cough, but 79% (n=27) hemograms had no characteristic changes, which, along with a low frequency of confirmation of the diagnosis by PCR 22% (n=4), made it difficult to diagnose whooping cough. Conclusion: children over 7 years of age may not have characteristic hematological changes and PCR diagnostics are insufficiently effective, which contributes to the spread of whooping cough in family foci.

April 20 to 26, 2020 – World Immunization Week

2020 ◽  
pp. 76-79
Author(s):  
Oksana Rybachok
Keyword(s):  
Western Europe ◽  
Whooping Cough ◽  
Developed Countries ◽  
World Health ◽  
Modern World ◽  
Pneumococcal Infections ◽  
Preventive Vaccination ◽  
The Russian Federation ◽  
Health Organization ◽  
The Third World

Infectious diseases in the modern world continue to claim millions of human lives despite the achievements of medicine. While in developed countries the main cause of death is cancer and diseases of the cardiovascular system, it is the infectious processes that occupy leading positions in the structure of mortality in the third world countries. About 1.7 million children die from infections that could have been avoided by vaccination according to the World Health Organization. In contrast to the countries of Western Europe, where preventive vaccinations for the population are carried out for a fee, preventive vaccination in the Russian Federation is funded by the state. Immunoprophylaxis includes not only prevention of 12 major infections included in the calendar of preventive vaccinations (diphtheria, polio, tetanus, whooping cough, tuberculosis, measles, rubella, mumps, hepatitis B, pneumococcal infections and haemophilus influenzae, influenza), but also vaccination against 17 additional infections in case of epidemiological indications.

Visnagin: A New Perspective of Medicinal Importance, Physiological Functions, Phytochemistry, Pharmacology and Analytical Aspects of Active Phytoconstituents of Ammi visnaga

2019 ◽  
Vol 9 (3) ◽  
pp. 197-206
Author(s):  
Kanika Patel ◽  
Mahfoozur Rahman ◽  
Vikas Kumar ◽  
Amita Verma ◽  
Dinesh Kumar Patel
Keyword(s):  
Angina Pectoris ◽  
Renal Colic ◽  
Whooping Cough ◽  
Analytical Techniques ◽  
Epileptic Seizures ◽  
Coronary Insufficiency ◽  
Ammi Visnaga ◽  
Low Blood Pressure ◽  
Annual Herb ◽  
New Perspective

Background: Ammi visnaga commonly known as ‘honey plant, tooth pick fruit, bisnaga and khella’ is an important plant of Apiaceae family. It is an annual herb found in Europe, Asia, North Africa, Latin America and in India. Carrot, parsnip, celery, coriander, anise, caraway, cumin, parsley and dill are some other plants of the same family i.e., ‘Apiaceae’. Fruits of Ammi visnaga (A. visnaga) are medicinally used as a strong photosensitizer, diaphoretic, carminative and antispasmodic agents. Objective: A. visnaga has been used for the treatment of bronchial asthma, coronary insufficiency, angina pectoris, psoriasis, renal colic and ureteric stones. Visnagin (C13H10O4) is a ‘furanochromone derivative’ which is the main active constituent’s of A. visnaga. Visnagin is used for the treatment of low blood-pressure, angina pectoris and kidney stone. It also has neuroprotective and antiinflammatory activity. Moreover, visnagin also treat whooping cough, ureter and bile duct, gall bladder and renal colic, tumors and epileptic seizures. Methods: This review summarizes data’s regarding the biological importance, pharmacological uses and medicinal importance of A. visnaga and their important active constituent’s “visnagin”. Moreover, the detailed pharmacological aspects of visnagin were presented in this review. Further various analytical techniques used for the extraction and isolation of visnagin were also discussed. Studies related to the pharmacological profile of the plants and their active phytoconstituents are one of the leading areas of research. Conclusion: This review will be beneficial to the scientific society to understand the importance of A. visnaga plant and their active constituents ‘visnagin’for the development of alternative tools to treat disorders in the future.

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