Post-mortem visualization of peroxisomes in rat and in human liver

1990 ◽  
Vol 22 (1) ◽  
pp. 36-44 ◽  
Author(s):  
D. De Craemer ◽  
M. Espeel ◽  
M. Langendries ◽  
R. B. H. Schutgens ◽  
T. Hashimoto ◽  
...  
Keyword(s):  
Human Liver ◽  
Post Mortem

VANILLINSÄURE ALS ENDPRODUKT DES ABBAUES VON ADRENALIN UND NORADRENALIN

1964 ◽  
Vol 45 (4) ◽  
pp. 641-646 ◽  
Author(s):  
Wilhelm Dirscherl ◽  
Betty Brisse
Keyword(s):  
Melting Point ◽  
Rat Liver ◽  
Vanillic Acid ◽  
Human Liver ◽  
Mandelic Acid ◽  
Post Mortem ◽  
The Third ◽  
Uv And Ir Spectra ◽  
Solvent Systems ◽  
Liver Homogenates

ABSTRACT Incubation of homogenates of rat liver or human liver with D,L-3-methoxy-4-hydroxy-mandelic acid yielded 3 diazopositive compounds. In addition to the starting material, vanillic acid could be isolated by means of column chromatography. It was identified by the shape of its crystals, micro melting point, mixed melting point, UV- and IR-spectra, and paper chromatography in 3 different solvent systems. The third substance has not yet been identified. Since acid hydrolysis does not result in any cleavage a conjugate can be ruled out. The yield of vanillic acid was about 12 per cent with rat liver, 3 per cent with post mortem liver and 7-8 per cent with liver obtained at operation. Vanillic acid is not further metabolized by liver homogenates and can also be considered as the final metabolite of adrenaline and noradrenaline in the human liver.

3-METHOXY-4-HYDROXY-BENZALDEHYD (VANILLIN) ALS METABOLIT VON ADRENALIN UND NORADRENALIN

1964 ◽  
Vol 47 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Helmut Thomas ◽  
Wilhelm Dirscherl
Keyword(s):  
Absorption Spectrum ◽  
Melting Point ◽  
Propionic Acid ◽  
Ir Spectrum ◽  
Vanillic Acid ◽  
Incubation Medium ◽  
Human Liver ◽  
Mandelic Acid ◽  
Post Mortem ◽  
Intermediary Product

ABSTRACT After incubation of 3-methoxy-4-hydroxy-mandelic acid, a metabolite of adrenaline and noradrenaline, with rat liver slices and slices of human liver obtained at post mortem, in addition to vanillic acid one intermediary product was detected by paper chromatography. In the system benzene-propionic acid-water (2:2:1) this showed the same RF-value as 3-methoxy-4-hydroxy-benzaldehyde (vanillin). In the deproteinized incubation medium the substance was precipitated as 2,4-dinitrophenylhydrazone, which after purification and recrystallisation was identified by melting point, absorption spectrum between 220 and 500 mμ, and IR-spectrum as 2,4-dinitrophenylhydrazone of vanillin.

Monooxygenase Activities of Human Liver, Lung, and Kidney Microsomes - A Study of 42 post mortem Cases

2009 ◽  
Vol 50 (5) ◽  
pp. 332-341 ◽  
Author(s):  
Sten W. Jakobsson ◽  
Richard T. Okita ◽  
Nell I. Mock ◽  
Bettie Sue S. Masters ◽  
L. Maximilian Buja ◽  
...  
Keyword(s):  
Human Liver ◽  
Post Mortem ◽  
Kidney Microsomes ◽  
Lung And Kidney

Gamma-Glutamyl Transferase: Applications in Forensic Pathology II: Histochemical Study of Post-Mortem Human Liver Samples

1987 ◽  
Vol 27 (3) ◽  
pp. 161-169 ◽  
Author(s):  
M. Piette ◽  
Ch. Devos ◽  
B. De Prest
Keyword(s):  
Liver Tissue ◽  
Human Liver ◽  
False Negative ◽  
Chronic Alcoholism ◽  
Forensic Autopsy ◽  
Hepatic Tissue ◽  
Gamma Glutamyl Transferase ◽  
Post Mortem ◽  
Gamma Glutamyl ◽  
Glutamyl Transferase

In part I of this paper the role of a serum gamma-glutamyl transferase (GGT) determination as a marker of chronic alcoholism in the course of a forensic autopsy was analysed and discussed. To enhance the reliability of this GGT determination in cadaver serum, the suitability of a histochemical method for detecting chronic alcoholism by means of this enzyme in human liver tissue was analysed. As a preliminary test, the post-mortem stability of the GGT enzyme was observed by storing liver samples in vitro. Subsequently, correlations between liver-staining and the degree of fatty liver degeneration and fibrosis (both ethanol-induced lesions) were searched for. A comparison was then made between selected groups of alcoholics and non-alcoholics. Finally, the correlation between serum GGT activity and liver tissue staining was made. We conclude that, keeping in mind the possibility of both false positive and false negative results, a histochemical test for GGT in hepatic tissue is a further tool for detecting chronic alcoholism at the forensic autopsy. The combined examination of GGT in serum and in liver tissue is emphasized.

Ultrastructural investigation of an adenoma of the pituitary post mortem

1987 ◽  
Vol 45 ◽  
pp. 622-623
Author(s):  
Shirley Siew ◽  
W. C. deMendonca
Keyword(s):  
Deleterious Effect ◽  
Anterior Lobe ◽  
Definitive Diagnosis ◽  
Pars Intermedia ◽  
Post Mortem ◽  
Close Apposition ◽  
Autopsy Material ◽  
Progressive Loss ◽  
Transmission Electron ◽  
Means Of Transmission

The deleterious effect of post mortem degeneration results in a progressive loss of ultrastructural detail. This had led to reluctance (if not refusal) to examine autopsy material by means of transmission electron microscopy. Nevertheless, Johannesen has drawn attention to the fact that a sufficient amount of significant features may be preserved in order to enable the establishment of a definitive diagnosis, even on “graveyard” tissue.Routine histopathology of the autopsy organs of a woman of 78 showed the presence of a well circumscribed adenoma in the anterior lobe of the pituitary. The lesion came into close apposition to the pars intermedia. Its architecture was more compact and less vascular than that of the anterior lobe. However, there was some grouping of the cells in relation to blood vessels. The cells tended to be smaller, with a higher nucleocytoplasmic ratio. The cytoplasm showed a paucity of granules. In some of the cells, it was eosinophilic.

Deactivation of anti-cancer drug letrozole to a carbinol metabolite by polymorphic cytochrome P450 2A6 in human liver microsomes

Xenobiotica ◽  
2009 ◽  
Vol 00 (00) ◽  
pp. 090901052053001-8
Author(s):  
K. Murai ◽  
H. Yamazaki ◽  
K. Nakagawa ◽  
R. Kawai ◽  
T. Kamataki
Keyword(s):  
Cytochrome P450 ◽  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Cancer Drug ◽  
Anti Cancer ◽  
Cytochrome P450 2A6

Post-mortem Peyer’s patches: Their potential application in forensic medicine

2009 ◽  
Vol 00 (00) ◽  
pp. 090513010017019-7
Author(s):  
Biagio Solarino ◽  
Giancarlo Di Vella ◽  
Thea Magrone ◽  
Felicita Jirillo ◽  
Angela Tafaro ◽  
...  
Keyword(s):  
Forensic Medicine ◽  
Potential Application ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Post Mortem

Author response for "Human liver‐derived MAIT cells differ from blood MAIT cells in their metabolism and response to TCR‐independent activation"

2020 ◽  
Author(s):  
Rajesh Lamichhane ◽  
Fran Munro ◽  
Thomas W. R. Harrop ◽  
Sara M. Harpe ◽  
Peter K. Dearden ◽  
...  
Keyword(s):  
Human Liver ◽  
Author Response ◽  
Mait Cells

Aneurysms in the history of medicine

VASA ◽  
2002 ◽  
Vol 31 (4) ◽  
pp. 281-286 ◽  
Author(s):  
Bollinger ◽  
Rüttimann
Keyword(s):  
History Of Medicine ◽  
Albert Einstein ◽  
Thomas Mann ◽  
Post Mortem ◽  
John Hunter ◽  
History Of

Die Geschichte des sackförmigen oder fusiformen Aneurysmas reicht in die Zeit der alten Ägypter, Byzantiner und Griechen zurück. Vesal 1557 und Harvey 1628 führten den Begriff in die moderne Medizin ein, indem sie bei je einem Patienten einen pulsierenden Tumor intra vitam feststellten und post mortem verifizierten. Weitere Eckpfeiler bildeten die Monographien von Lancisi und Scarpa im 18. bzw. beginnenden 19. Jahrhundert. Die erste wirksame Therapie bestand in der Kompression des Aneurysmasacks von außen, die zweite in der Arterienligatur, der John Hunter 1785 zum Durchbruch verhalf. Endoaneurysmoraphie (Matas) und Umhüllung mit Folien wurden breit angewendet, bevor Ultraschalldiagnostik und Bypass-Chirurgie Routineverfahren wurden und die Prognose dramatisch verbesserten. Die diagnostischen und therapeutischen Probleme in der Mitte des 20. Jahrhunderts werden anhand von zwei prominenten Patienten dargestellt, Albert Einstein und Thomas Mann, die beide im Jahr 1955 an einer Aneurysmaruptur verstarben.

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