Large scale cultivation of a free-living nematode (Caenorhabditis elegans)

1976 ◽  
Vol 32 (8) ◽  
pp. 1087-1088 ◽  
Author(s):  
J. R. Vanfleteren
Keyword(s):  
Caenorhabditis Elegans ◽  
Large Scale ◽  
Nematode Caenorhabditis Elegans ◽  
Free Living ◽  
Free Living Nematode ◽  
Large Scale Cultivation

Large Scale Cultivation of the Free Living Nematode Caenorhabditis elegans

1994 ◽  
Vol 12 (1) ◽  
pp. 51-54 ◽  
Author(s):  
Kodzo Gbewonyo ◽  
Susan P. Rohrer ◽  
Leonard Lister ◽  
Bruce Burgess ◽  
Doris Cully ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Large Scale ◽  
Nematode Caenorhabditis Elegans ◽  
Free Living ◽  
Free Living Nematode ◽  
Large Scale Cultivation

Nutritional requirements for pantothenate, pantethine or coenzyme A in the free-living nematode Caenorhabditis elegans

Nematology ◽  
2005 ◽  
Vol 7 (5) ◽  
pp. 761-766 ◽  
Author(s):  
Nancy Lu ◽  
Rekha Balachandar
Keyword(s):  
Caenorhabditis Elegans ◽  
Coenzyme A ◽  
Active Form ◽  
Calcium Pantothenate ◽  
Axenic Medium ◽  
Nematode Caenorhabditis Elegans ◽  
Free Living ◽  
C Elegans ◽  
Free Living Nematode ◽  
Mild Toxicity

AbstractCaenorhabditis elegans is a free-living nematode cultured in an axenic medium, the Caenorhabditis elegans Maintenance Medium (CeMM), which contains B-vitamins, salts, amino acids, nucleic acid substituents, growth factors and glucose as an energy source. After initial experiments established that either pantothenate or pantethine would satisfy the vitamin B5 requirement in C. elegans, reproduction in the nematodes was measured in eight equimolar concentrations of calcium pantothenate, pantethine or coenzyme A. The optimal levels for pantothenate were found to be 7.5, 30 and 120 μg ml−1. The optimal levels for pantethine and coenzyme A were found to be 35 μg ml−1 and 100 μg ml−1, respectively. Among the three compounds, coenzyme A (at 100 μg/ml) supported a significantly greater population growth and, perhaps, is a more metabolically active form. Mild toxicity was demonstrated for pantothenate at 480μg ml−1, pantethine at 560 and 140 μg ml−1, and coenzyme A was found to exhibit toxicity at 410 and 1700 μg ml−1. Based on our results, we recommend that in the future the CeMM could be supplemented with pantothenate (7.5 μg ml−1) alone.

scholarly journals Novel expression of Haemonchus contortus vaccine candidate aminopeptidase H11 using the free-living nematode Caenorhabditis elegans

2013 ◽  
Vol 44 (1) ◽  
pp. 111 ◽  
Author(s):  
Brett Roberts ◽  
Aristotelis Antonopoulos ◽  
Stuart M Haslam ◽  
Alison J Dicker ◽  
Tom N McNeilly ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Haemonchus Contortus ◽  
Vaccine Candidate ◽  
Nematode Caenorhabditis Elegans ◽  
Free Living ◽  
Free Living Nematode

Responses of dauerlarvae of Caenorhabditis elegans (Nematoda: Rhabditidae) to thermal stress and oxygen deprivation

1978 ◽  
Vol 56 (8) ◽  
pp. 1786-1791 ◽  
Author(s):  
Gary L. Anderson
Keyword(s):  
Thermal Stress ◽  
Caenorhabditis Elegans ◽  
Developmental Stage ◽  
Thermal Tolerance ◽  
Oxygen Deprivation ◽  
Behavior Changes ◽  
Nematode Caenorhabditis Elegans ◽  
Free Living ◽  
Anaerobic Stress ◽  
Free Living Nematode

Larval forms of the free-living nematode Caenorhabditis elegans possess the ability to enter a developmental stage which is thought to be specialized for survival in harsh environmental conditions, i.e. the dauerlarval stage. In this study the responses of dauerlarvae to thermal stress and oxygen deprivation are investigated. Oxygen consumption of dauerlarvae is less sensitive to temperature change that that of adults, with Q10 values of 1.7 and 2.6 respectively. The upper thermal tolerance limit of dauerlarvae is also different from that of adults; dauerlarvae survive approximately three times longer than adults when exposed to 37 °C. In addition to differences in thermal tolerance, dauerlarvae survive longer under anaerobic conditions than adults, 7 days and 2 days respectively. On recovery from anaerobic stress dauerlarvae exhibit behavior changes which are suggestive of emergence from the dauerlarval stage. The responses of dauerlarvae to thermal stress and oxygen deprivation appear to be important aspects of the specialization for survival in this facultative developmental stage.

The buccal capsule of Caenorhabditis elegans (Nematoda: Rhabditoidea): an ultrastructural study

1981 ◽  
Vol 59 (10) ◽  
pp. 1952-1961 ◽  
Author(s):  
K. A. Wright ◽  
J. N. Thomson
Keyword(s):  
Caenorhabditis Elegans ◽  
Body Wall ◽  
Buccal Capsule ◽  
Nematode Caenorhabditis Elegans ◽  
Free Living ◽  
Dense Granules ◽  
Free Living Nematode ◽  
Section Electron ◽  
Serial Section Electron Microscopy ◽  
Esophageal Muscle

The buccal capsule of the free-living nematode Caenorhabditis elegans has been analysed by serial section electron microscopy. Whereas the regions classically identified in the rhabditid buccal capsule can be distinguished, the cuticle lining does not constitute separate cuticular plates, but rather, structural–functional differentiations within a cuticle continuous with that of the esophagus. Only the lip region (cheilostom) is lined by body wall cuticle. The prostom cuticle is underlain by two rings of syncytial arcade cytoplasm connected to nine cell bodies. The mesostom cuticle is underlain by the nonmuscular epithelial cells of the esophagus, whereas the cuticle of the metastom and telostom is underlain by esophageal muscle cells m1 and m2. During moulting, buccal cuticle is produced later than body cuticle and its formation is characterized by accumulation of dense granules in both arcade and esophageal cytoplasm. It is concluded that the buccal capsule should be considered as "astomatous" in the terminology of K. A. Wright.

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