The influence of cyclic nucleotides (c-AMP and c-GMP) on the nitrous blue tetrazolium reduction by human polymorphonuclear leucocytes

1975 ◽  
Vol 31 (1) ◽  
pp. 118-120 ◽  
Author(s):  
Z. Spirer ◽  
V. Zakuth ◽  
A. Golander ◽  
N. Bogair
Keyword(s):  
Cyclic Nucleotides ◽  
Polymorphonuclear Leucocytes ◽  
Blue Tetrazolium ◽  
Tetrazolium Reduction

Ultrastructure and sites of tetranitro-blue tetrazolium reduction of Lactobacillus casei

1968 ◽  
Vol 14 (8) ◽  
pp. 823-827 ◽  
Author(s):  
Joseph P. Brown ◽  
Mercedes R. Edwards ◽  
Paul J. VanDemark
Keyword(s):  
Plasma Membrane ◽  
Normal Control ◽  
Electron Micrographs ◽  
Lactobacillus Casei ◽  
Reduction Product ◽  
Lactobacillus Species ◽  
Blue Tetrazolium ◽  
Ultrathin Sections ◽  
Ultrastructural Characteristics ◽  
Tetrazolium Reduction

Cells of two strains of Lactobacillus casei incubated for 2–10 h in the presence of tetranitro-blue tetrazolium (TNBT) and mannitol were studied in electron micrographs of ultrathin sections. The reduction product tetranitro-blue diformazan (TNBF) was found to be predominantly associated with the plasma membrane and its derivatives. Untreated cells (normal control), as well as cells incubated with mannitol in absence of TNBT (dye control), or with omission of mannitol (substrate control) displayed ultrastructural characteristics, in general, similar to those previously reported for other Gram-positive bacilli, particularly Lactobacillus species.

scholarly journals THE EFFECT OF MENADIONE AND PHENAZINE METHOSULFATE ON THE TETRAZOLIUM REDUCTION SYSTEM UNDER HISTOCHEMICAL CONDITIONS

1964 ◽  
Vol 12 (11) ◽  
pp. 797-804 ◽  
Author(s):  
T. HASHIMOTO ◽  
J. S. KALUZA ◽  
M. S. BURSTONE
Keyword(s):  
Nitro Blue Tetrazolium ◽  
Soluble Factor ◽  
Phenazine Methosulfate ◽  
Frozen Sections ◽  
Hydrogen Acceptor ◽  
Variable Effect ◽  
Isocitric Dehydrogenase ◽  
Blue Tetrazolium ◽  
Fresh Frozen ◽  
Tetrazolium Reduction

The influence of phenazine methosulfate and menadione on the activity of DPNH- and TPNH-tetrazolium reductase, succinic, lactic, and triphosphopyridine nucleotide-linked isocitric dehydrogenase were studied with reference to applied histochemistry. Fresh frozen sections of various tissues were employed in conjunction with nitro blue tetrazolium as the hydrogen acceptor. Phenazine methosulfate was found to produce a variable effect upon staining, enhancing it or suppressing it according to incubation conditions. Suppression was most pronounced with higher concentrations. Menadione had no suppressive influence and was useful in increasing tetrazolium reduction. Its usefulness is, however, limited unless a soluble factor, possibly DT-(DPNH-TPNH)-diaphorase, can be prevented from diffusing into the medium, and "nothing dehydrogenase" is selectively inactivated.

scholarly journals TETRANITRO-BLUE TETRAZOLIUM REDUCTION IN BACILLUS SUBTILIS

1965 ◽  
Vol 27 (1) ◽  
pp. 237-241 ◽  
Author(s):  
W. Leene ◽  
Woutera van Iterson
Keyword(s):  
Bacillus Subtilis ◽  
Blue Tetrazolium ◽  
Tetrazolium Reduction
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