Elaunin fibres in the basement membrane of sweat gland secretory coil are rich in disulfide-groups

1979 ◽  
Vol 35 (4) ◽  
pp. 538-539 ◽  
Author(s):  
P. Böck
Keyword(s):  
Basement Membrane ◽  
Sweat Gland ◽  
Elaunin Fibres ◽  
Secretory Coil

Electrophysiologically distinct cell types in human sweat gland secretory coil

1992 ◽  
Vol 262 (2) ◽  
pp. C287-C292 ◽  
Author(s):  
M. M. Reddy ◽  
P. M. Quinton
Keyword(s):  
Sweat Gland ◽  
Basolateral Membrane ◽  
Cell Types ◽  
Membrane Potentials ◽  
Adrenergic Stimulation ◽  
Beta Adrenergic ◽  
Human Sweat ◽  
Distinct Cell ◽  
Secretory Coil ◽  
I Cells

The human sweat gland secretory coil consists of three histologically distinct cell types: myoepithelial (ME), light (or clear), and dark cells. The electrophysiological properties of all these cells are poorly defined. Employing electrophysiological techniques, we report distinct pharmacological responses of three different cell types from freshly isolated human sweat gland secretory coil. The superficial ME cells are characterized by 1) spontaneous depolarizing spikes (2 to 50 mV), 2) high cell membrane potentials [Vm = -68.6 +/- 3.9 (SE) mV; n = 21], 3) a K(+)-selective cell membrane (slope response = 54.2 +/- 6.7 mV per decade K+ concentration; n = 4), 4) depolarizing responses to cholinergic agonist mecholyl (delta Vm = 29.1 +/- 3.1 mV, n = 21), and 5) insensitivity to beta-adrenergic stimulation (n = 12). Two other types of cells, presumably secretory, were also observed. We arbitrarily labeled these cells as beta-adrenergic sensitive (beta-S) and beta-adrenergic insensitive (beta-I) cells based on their respective sensitivity to isoproterenol (IPR), a beta-adrenomimetic. Properties of the beta-S cells include 1) relatively higher basolateral membrane potentials (Vm = -57.3 +/- 3.1 mV; n = 13), 2) depolarizing responses to IPR (delta Vm = 16.8 +/- 2.6 mV; n = 9) inhibitable by the beta-adrenergic antagonist propranolol, and 3) hyperpolarizing responses to mecholyl (delta Vm = -21.8 +/- 2.0 mV; n = 13). The beta-I cells are characterized by 1) low basolateral membrane potentials (Vm = -23.6 +/- 2.1 mV; n = 16), 2) insensitivity to beta-adrenergic stimulation, and 3) hyperpolarizating responses to mecholyl (delta Vm = -16.1 +/- 2.1 mV; n = 16).

Interleukin-1 alpha in human sweat is functionally active and derived from the eccrine sweat gland

1994 ◽  
Vol 266 (3) ◽  
pp. R950-R959 ◽  
Author(s):  
K. Sato ◽  
F. Sato
Keyword(s):  
Sweat Gland ◽  
Plasma Membranes ◽  
Sweat Rate ◽  
Interleukin 1 ◽  
Biologically Active ◽  
Sweat Test ◽  
Cultured Fibroblasts ◽  
Human Sweat ◽  
The Mean ◽  
Secretory Coil

We wished to establish the presence of interleukin-1 (IL-1) in human sweat (5) and clarify its origin and mechanism of secretion. IL-1 alpha concentration ([IL-1 alpha]) in clean sweat from the back increased with the sweat rate, plateauing at the maximal sweat rate ([IL-1 alpha]max). The mean [IL-1 alpha]max was 545 pg/ml (n = 17) for men and 1,324 pg/ml for women in back sweat. The mean [IL-1 alpha]max for axillary sweat in men was 1,568 (n = 6). Palmar sweat was 9.2 ng/ml (n = 5) for IL-1 alpha and 7.9 ng/ml for IL-1 beta. [IL-1 alpha]max decreased to one-third that of the first sweat test, when second sauna sweat tests were conducted after 2 h of continuous sweating on the same day. Western blot analysis of the purified sweat IL-1 alpha fraction revealed bands at 17, 29, and 33 kDa. Immunoreactive IL-1 alpha was localized mainly in the secretory coil lumen, intercellular canaliculi, cytoplasm, mitochondria, and near plasma membranes. Polymerase chain reaction revealed the presence of IL-1 alpha mRNA in the sweat gland and in cultured human eccrine secretory coil cells. Both sweat IL-1 alpha and human recombinant IL-1 alpha at 500 pg/ml strongly stimulated interleukin-6 and interleukin-8 production in cultured fibroblasts. We conclude that the IL-1 alpha-like immunoreactive substance in sweat is IL-1 alpha itself, is derived from the sweat gland, and is biologically active at concentrations normally present in fresh sweat.

scholarly journals Thigmotropism of Malignant Melanoma Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Pascale Quatresooz ◽  
Claudine Piérard-Franchimont ◽  
Fanchon Noël ◽  
Gérald E. Piérard
Keyword(s):  
Malignant Melanoma ◽  
Basement Membrane ◽  
Growth Model ◽  
Growth Phase ◽  
Sweat Gland ◽  
Cell Shape ◽  
Hair Follicles ◽  
Perivascular Space ◽  
Primary Neoplasm ◽  
Contact Sensing

During malignant melanoma (MM) progression including incipient metastasis, neoplastic cells follow some specific migration paths inside the skin. In particular, they progress along the dermoepidermal basement membrane, the hair follicles, the sweat gland apparatus, nerves, and the near perivascular space. These features evoke the thigmotropism phenomenon defined as a contact-sensing growth of cells. This process is likely connected to modulation in cell tensegrity (control of the cell shape). These specifically located paucicellular aggregates of MM cells do not appear to be involved in the tumorigenic growth phase, but rather they participate in the so-called “accretive” growth model. These MM cell collections are often part of the primary neoplasm, but they may, however, correspond to MM micrometastases and predict further local overt metastasis spread.

Secretion of Ions and Pharmacological Responsiveness in the Mouse Paw Sweat Gland

1994 ◽  
Vol 86 (2) ◽  
pp. 133-139 ◽  
Author(s):  
K. Sato ◽  
S. Cavallin ◽  
K. T. Sato ◽  
F. Sato
Keyword(s):  
Cyclic Amp ◽  
Sweat Gland ◽  
Sweat Rate ◽  
Eccrine Sweat Gland ◽  
Sweat Secretion ◽  
Cl Channels ◽  
Functional Features ◽  
Secretory Coil ◽  
Rat Paw

1. Some of the basic functional features of the mouse paw eccrine sweat gland were delineated to allow comparison with those of transgenic mice in the future. 2. The mouse sweat secretory coil responds to methacholine, elaborating a K+-rich (> 120 mmol/l), Na+-poor (< 70 mmol/l) primary fluid as does the rat paw sweat gland, as previously reported. The methacholine-induced sweat rate increases with age in parallel with the growth of the sweat gland over the first 6 weeks of life. 3. The sweating response to cyclic AMP-elevating agents, such as isoprenaline or forskolin, is as much as 40% of the methacholine-induced sweat rate at 1 week of age, but falls to 10% by 6 weeks of age despite the fact that the agonist-induced tissue accumulation of cyclic AMP expressed on a per μg of protein basis triples with age over the same period. 4. A marked K+ outflux was also noted in response to methacholine and a small K+ outflux was seen in response to cyclic AMP-elevating agonists in super-fused adult mouse secretory coils in vitro. 5. Since sweat secretion is usually associated with activation of either K+ channels or Cl− channels or both, and since the sweating occurred in response to cyclic AMP-elevating agonists, we speculate that the cyclic AMP-activated Cl− channels (the mouse version of the cystic fibrosis transmembrane conductance regulator) may also occur in the mouse sweat gland, but that the degree of their expression may be influenced by the age of the mice.

Functional and morphological changes in the eccrine sweat gland with heat acclimation

1990 ◽  
Vol 69 (1) ◽  
pp. 232-236 ◽  
Author(s):  
F. Sato ◽  
M. Owen ◽  
R. Matthes ◽  
K. Sato ◽  
C. V. Gisolfi
Keyword(s):  
Relative Humidity ◽  
Heat Tolerance ◽  
Sweat Gland ◽  
Unit Length ◽  
Heat Acclimation ◽  
Tolerance Test ◽  
Sweat Glands ◽  
Before And After ◽  
Secretory Coil

Three adult male patas monkeys (11-15 kg) were heat acclimated by continuous exposure to an ambient temperature of 33 +/- 1 degree C at 13% relative humidity for 9 mo. During the last month, they were also exposed to 45 degrees C at 10% relative humidity for 4 h/day and 5 days/wk. Before and after 3 wk of acclimation, the animals were given a heat-tolerance test in which rectal (Tre) and mean skin (Tsk) temperatures, heart rate, and sweat rate (msw) were monitored during a 90-min exposure to 45 degrees C heat with 24% relative humidity under lenperone (1.0-1.4 mg/kg im) tranquilization. Maximal in vivo msw was also determined in response to subcutaneous injections (1 and 10% solutions) of methacholine (MCh). Before and after 9 wk and 9 mo of acclimation, sweat glands were dissected from biopsy specimens of the lateral calf, cannulated, and stimulated in vitro with MCh. Morphological measurements of isolated tubules were compared with maximal secretory rates produced by MCh stimulation. Three weeks of acclimation 1) reduced Tre and Tsk and increased msw during the heat tolerance test and 2) significantly increased maximal msw in response to MCh stimulation. Acclimation also increased (P less than 0.05) sweat gland size, as measured by tubular length and tubular volume. Maximal in vitro msw produced by MCh stimulation and msw per unit length of secretory coil also increased significantly. We conclude that heat acclimation increases the size of eccrine sweat glands and that these larger glands produce more sweat. They are also more efficient because they produce more sweat per unit length of secretory coil.

scholarly journals Diagnostic Value of Linear Fluorescence Along the Basement Membrane of Sweat Gland Ducts in Bullous Pemphigoid

2017 ◽  
Vol 97 (5) ◽  
pp. 622-626 ◽  
Author(s):  
I Bağcı ◽  
O Horváth ◽  
E Schmidt ◽  
T Ruzicka ◽  
M Sárdy
Keyword(s):  
Basement Membrane ◽  
Bullous Pemphigoid ◽  
Sweat Gland ◽  
Diagnostic Value

Matrigel basement membrane matrix induces eccrine sweat gland cells to reconstitute sweat gland-like structures in nude mice

2015 ◽  
Vol 332 (1) ◽  
pp. 67-77 ◽  
Author(s):  
Haihong Li ◽  
Lu Chen ◽  
Shaopeng Zeng ◽  
Xuexue Li ◽  
Xiang Zhang ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Nude Mice ◽  
Sweat Gland ◽  
Eccrine Sweat Gland ◽  
Gland Cells ◽  
Membrane Matrix ◽  
Eccrine Sweat

Primary Culture of Epithelial Cells from the Secretory Coil and the Reabsorptive Duct of the Human Eccrine Sweat Gland

1985 ◽  
Vol 69 (s12) ◽  
pp. 82P-83P
Author(s):  
Catherine M. Lee ◽  
F. Carpenter ◽  
T. Kealey ◽  
K.G.M.M. Alberti
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Sweat Gland ◽  
Eccrine Sweat Gland ◽  
Secretory Coil ◽  
Eccrine Sweat

Evidence of two distinct epithelial cell types in primary cultures from human sweat gland secretory coil

1992 ◽  
Vol 262 (4) ◽  
pp. C891-C898 ◽  
Author(s):  
M. M. Reddy ◽  
C. L. Bell ◽  
P. M. Quinton
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Sweat Gland ◽  
Primary Cultures ◽  
Membrane Potentials ◽  
Secretory Cells ◽  
Beta Adrenergic ◽  
Human Sweat ◽  
Secretory Coil ◽  
I Cells

The human sweat gland secretory coil (SC) is comprised of myoepithelial (ME) and two types of secretory epithelial cells. The secretory cells include beta-adrenergic-sensitive (beta-S) cells [responsive to the beta-adrenergic agonist isoproterenol (IPR)] and beta-adrenergic insensitive (beta-I) cells. We have grown segments of SC in primary culture and found that under the conditions described here, only epithelial cells form outgrowths as indicated by morphological and physiological properties. As in the native SC epithelium, the secretory cells in primary culture were comprised of polygonal epithelial cells with a characteristic hyperpolarization of cell potentials (Vm) to cholinergic stimulation by mecholyl (magnitude of change of Vm = delta Vm = 21.5 +/- 1.3 mV, mean +/- SE, n = number of cells = 44). We have found both beta-S and beta-I cells as determined by unstimulated membrane potentials, sensitivity to IPR, and K+ conductance (GK+). The frequency distribution of unstimulated cells indicated two distinct populations of cells, one with high membrane potentials (Vm = -63 +/- 2.6 mV), which correlated with beta-S cells, and a second with low membrane potentials (Vm = -22 +/- 1.5 mV), which correlated with the beta-I cells. IPR depolarized the Vm of beta-S cells (delta Vm = 11.0 +/- 0.8 mV, n = 25) without affecting the Vm of beta-I cells.(ABSTRACT TRUNCATED AT 250 WORDS)

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