Primary Culture of Epithelial Cells from the Secretory Coil and the Reabsorptive Duct of the Human Eccrine Sweat Gland

1985 ◽  
Vol 69 (s12) ◽  
pp. 82P-83P
Author(s):  
Catherine M. Lee ◽  
F. Carpenter ◽  
T. Kealey ◽  
K.G.M.M. Alberti
1995 ◽  
Vol 37 (4) ◽  
pp. 507-509
Author(s):  
ICHIRO OHNO ◽  
KAZUKO SHINODA ◽  
KEIKO TSUGAWA ◽  
NOBORU TAKIZAWA ◽  
NOBORU TANIGUCHI ◽  
...  

1992 ◽  
Vol 262 (4) ◽  
pp. C891-C898 ◽  
Author(s):  
M. M. Reddy ◽  
C. L. Bell ◽  
P. M. Quinton

The human sweat gland secretory coil (SC) is comprised of myoepithelial (ME) and two types of secretory epithelial cells. The secretory cells include beta-adrenergic-sensitive (beta-S) cells [responsive to the beta-adrenergic agonist isoproterenol (IPR)] and beta-adrenergic insensitive (beta-I) cells. We have grown segments of SC in primary culture and found that under the conditions described here, only epithelial cells form outgrowths as indicated by morphological and physiological properties. As in the native SC epithelium, the secretory cells in primary culture were comprised of polygonal epithelial cells with a characteristic hyperpolarization of cell potentials (Vm) to cholinergic stimulation by mecholyl (magnitude of change of Vm = delta Vm = 21.5 +/- 1.3 mV, mean +/- SE, n = number of cells = 44). We have found both beta-S and beta-I cells as determined by unstimulated membrane potentials, sensitivity to IPR, and K+ conductance (GK+). The frequency distribution of unstimulated cells indicated two distinct populations of cells, one with high membrane potentials (Vm = -63 +/- 2.6 mV), which correlated with beta-S cells, and a second with low membrane potentials (Vm = -22 +/- 1.5 mV), which correlated with the beta-I cells. IPR depolarized the Vm of beta-S cells (delta Vm = 11.0 +/- 0.8 mV, n = 25) without affecting the Vm of beta-I cells.(ABSTRACT TRUNCATED AT 250 WORDS)


1995 ◽  
Vol 57 (2) ◽  
pp. 187-190 ◽  
Author(s):  
Hans Hilmar Goebel ◽  
Irene Warlo ◽  
Thomas Klockgether ◽  
Klaus Harzer

2011 ◽  
Vol 294 (9) ◽  
pp. 1525-1531 ◽  
Author(s):  
Xia Lei ◽  
Bo Liu ◽  
Jinjin Wu ◽  
Yuangang Lu ◽  
Yadong Yang

2012 ◽  
Vol 132 (8) ◽  
pp. 1967-1976 ◽  
Author(s):  
Gabriella Czifra ◽  
Attila G. Szöllősi ◽  
Balázs I. Tóth ◽  
Julien Demaude ◽  
Charbel Bouez ◽  
...  

1973 ◽  
Vol 107 (1) ◽  
pp. 94-96 ◽  
Author(s):  
G. Panet-Raymond

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