Orientation of Giemsa C-bands in interphase cells of Allium cepa L.

1977 ◽  
Vol 33 (4) ◽  
pp. 432-433 ◽  
Author(s):  
S. C. Roy ◽  
S. Ghosh
2014 ◽  
Vol 71 (3) ◽  
pp. 211-221 ◽  
Author(s):  
Agnieszka Majewska ◽  
Mirosława Furmanowa ◽  
Kazimierz Głowniak ◽  
Joanna Guzewska ◽  
Alicja Zobel ◽  
...  

We investigated the influence of extract from <em>Taxus baccata</em> var. Elegantissima (TbE) shoots in 1:8 dilution, containing paclitaxel in concentration of 81,6 µg/g fresh mass on ultrastructure and tubulin cytoskeleton of meristematic cells of <em>Allium cepa</em> L. root tips. Incubation time 3, 6, 12 and 24 h was followed with postincubation in water for 12 and 24 h. During shorter incubation (till 12 h) the surface of the cell nuclei decreased and chromatin became condensed (in comparison to control) but after 24 h the average surface increased and chromatin condensation decreased. In the course of incubation the average size of plastids and vacuoles increased. Moreover, after treatment mitochondria and plastids showed degradation of ultrastructure, which was reversed after 12 h of postincubation. Immunocytochemical assays demonstrated that in the course of incubation in the ThE extract, the tubulin cytoskeleton became partially disorganised. In most interphase cells, cortical microtubules (MTs) lost their oval transverse orientation. The preprophase band (PPB) position in the cell was often asymmetrical. The MTs array of the karyokinetic spindle and phragmoplast was also disturbed. These alterations were completely reversed during postincubation.


2020 ◽  
Vol 169 ◽  
pp. 01012
Author(s):  
Oksana Cherednichenko ◽  
Serikbay Nuraliev ◽  
Bakytzhan Bekmanov ◽  
Anastassiya Pilugina ◽  
Leila Dzhansugurova ◽  
...  

Pesticides and persistent organic pollutants (POPs) are a serious environmental problem for almost all countries of the world. The toxic and mutagenic activity of samples of natural water and water extracts of soils collected near the storage sites of old and prohibited pesticides located in the villages of Kyzylkairat, Amangeldy, Beskainar, Belbulak, Enbekshi. Territories of comparison Basshi village (Altyn Emel National Park) and near farmland, where pesticides were used in the past (Taukaraturk village, Almaty region) was investigated. Allium cepa L. was used as a biological test system. The mitotic activity and the frequency of disturbances at different stages of mitosis in onion root meristem were evaluated. Chemical analysis showed that in all soil samples the total content of 24 POPsPesticides exceeds the maximum permissible concentration (MPC). It was established that the level of mitotic activity of Allium cepa cells in all samples was slightly reduced compared with the control. In soil samples, the frequency of micronuclei in interphase cells (0.22-3.25%) is higher than in water samples (0.36-0.99%) (control 0.41%). The revealed increased level of cytogenetic disorders correlates with the total content of organochlorine pesticides.


1983 ◽  
Vol 25 (5) ◽  
pp. 478-486 ◽  
Author(s):  
Catharine P. Fussell

The arrangement of telomeres in eight types of differentiated Allium cepa L. interphase cells was studied to find whether the distribution pattern varies with differentiation as centromere distribution appears to in differentiated cells of mice and Triturus vulgaris L. The results show that telomere positions and groupings in A. cepa are essentially the same in differentiated and meristematic interphase nuclei; telomeres, which are roughly paired, are arranged in a telophase configuration along one side of the nucleus. Thus telomeres appear to maintain the same basic arrangement in differentiated and in meristematic cells. Comparison of chromosome arm lengths and interphase telomere positions suggests that interphase telomere arrangements are a function of chromosome arm lengths at the time the nuclear envelope forms. Such an arrangement would place homologous telomeres in the same latitudinal band of the interphase nucleus.


2018 ◽  
Vol 481 (1) ◽  
pp. 99-103
Author(s):  
А.Я. Болсуновский ◽  
Д.В. Дементьев ◽  
Е.М. Иняткина ◽  
Ю.В. Кладько ◽  
М.В. Петриченков ◽  
...  
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