Ultrastructural changes in dog thyroid follicular cells elicit by concanavalin A in vitro

1977 ◽  
Vol 33 (11) ◽  
pp. 1493-1495 ◽  
Author(s):  
J. A. Fernandez-Pol ◽  
J. P. Binette ◽  
M. T. Hays
Keyword(s):  
Concanavalin A ◽  
Ultrastructural Changes ◽  
Follicular Cells ◽  
Thyroid Follicular Cells

Ultrastructural Changes Caused by Plant Cytokxnins on Dog Thyroid Follicular Cells “In Vitro”

1976 ◽  
Vol 34 ◽  
pp. 174-175
Author(s):  
J. A. Fernandez-Pol
Keyword(s):  
Cyclic Amp ◽  
Ultrastructural Changes ◽  
Follicular Cells ◽  
Animal Cells ◽  
Thyroid Follicular Cells ◽  
In Vitro Response ◽  
Growth Promoting ◽  
Regulatory Effects ◽  
Derivatives Of

Plant cytokinins are a group of growth promoting hormones with pronounced metabolic and regulatory effects not only on plants but also on microbial and animal cells (l). The effects of the N6-substituted derivatives of adenine, especially Zeatin (trans-6-(4-hydroxy-3-methylbut-2-enyl)- aminopurine) and Kinetin (6-furfurylaminopurine) on ultrastructure and on the in vitro response to Thyrotropin (TSH) or 8Br-cyclic AMP were studied in dog thyroid follicular cells.Thyroid slices were incubated in the presence of Zeatin or Kinetin with and without TSH or 8Br-cyclic AMP, after 30min. prior incubation in Earle's solution. Effects on ultrastructure were observed after 30min. to 6hrs. of incubation. The thyroid slices after removal from incubation flasks were fixed immediately in 3%glutaraldehyde-formalin in 0.05M phosphate buffer. Specimens were then treated as described elsewhere (2). Observations were performed with a Siemens IA electron microscope.

Autologous, Orthotopic Thyroid Follicular Cell Transplantation: A Surgical Component of Ex Vivo Somatic Gene Therapy

1993 ◽  
Vol 108 (1) ◽  
pp. 51-62 ◽  
Author(s):  
Bert W. O'Malley ◽  
Milton J. Finegold ◽  
Fred D. Ledley
Keyword(s):  
Gene Therapy ◽  
Ex Vivo ◽  
Genetic Material ◽  
Genetically Engineered ◽  
Follicular Cells ◽  
Somatic Gene Therapy ◽  
Orthotopic Transplantation ◽  
Somatic Gene ◽  
Thyroid Follicular Cells

Ex vivo strategies for somatic gene therapy involve the harvest of primary cells from patients, the transfer of novel genetic material into these cells in cell culture, and reimplantation of the genetically engineered cells back into patients. In consideration of methods for targeting somatic gene therapy to the thyroid, we have studied the autologous, orthotopic transplantation of thyroid follicular cells in a canine model. Using the fluorescent dye Dil, we were able to stain follicular cells in vitro before transplantation and then follow the pattern of engraftment through histologic sectioning and microscopy up to 14 days after transplantation. The transplantations involved the direct injection of intact and disrupted follicles into a remaining thyroid lobe after cell harvest from the contralateral lobe. We also demonstrated engraftment of individual follicular cells recovered from primary monolayer cultures. Histologic studies revealed the presence of transplanted cells and follicles as well as focal regions of granulomatous reaction in close relation to the engrafted material. These studies demonstrate the feasibility of autologous, orthotopic transplantation of thyroid follicular cells. This method is an essential component of ex vivo strategies for targeting somatic gene therapy to the thyroid gland.

scholarly journals RADIOAUTOGRAPHIC STUDY OF IN VIVO AND IN VITRO INCORPORATION OF FUCOSE-3H INTO THYROGLOBULIN BY RAT THYROID FOLLICULAR CELLS

1971 ◽  
Vol 49 (3) ◽  
pp. 856-882 ◽  
Author(s):  
A. Haddad ◽  
Meredith D. Smith ◽  
Annette Herscovics ◽  
N. J. Nadler ◽  
C. P. Leblond
Keyword(s):  
Golgi Apparatus ◽  
Side Chains ◽  
Follicular Cells ◽  
Thyroid Follicular Cells ◽  
Apical Vesicles ◽  
Electron Microscopes ◽  
Rat Thyroid ◽  
Silver Grains

The incorporation of fucose-3H in rat thyroid follicles was studied by radioautography in the light and electron microscopes to determine the site of fucose incorporation into the carbohydrate side chains of thyroglobulin, and to follow the migration of thyroglobulin once it had been labeled with fucose-3H. Radioautographs were examined quantitatively in vivo at several times after injection of fucose-3H into rats, and in vitro following pulse-labeling of thyroid lobes in medium containing fucose-3H. At 3–5 min following fucose-3H administration in vivo, 85% of the silver grains were localized over the Golgi apparatus of thyroid follicular cells. By 20 min, silver grains appeared over apical vesicles, and by 1 hr over the colloid. At 4 hr, nearly all of the silver grains had migrated out of the cells into the colloid. Analysis of the changes in concentration of label with time showed that radioactivity over the Golgi apparatus increased for about 20 min and then decreased, while that over apical vesicles increased to reach a maximum at 35 min. Later, the concentration of label over the apical vesicles decreased, while that over the colloid increased. Similar results were obtained in vitro. It is concluded that fucose, which is located at the end of some of the carbohydrate side chains, is incorporated into thyroglobulin within the Golgi apparatus of thyroid follicular cells, thereby indicating that some of these side chains are completed there. Furthermore, the kinetic analysis demonstrates that apical vesicles are the secretion granules which transport thyroglobulin from the Golgi apparatus to the apex of the cell and release it into the colloid.

scholarly journals Ultrastructural changes in the thyroid follicular cells following encephalectomy or hypophysectomy in fetal rats.

1987 ◽  
Vol 49 (3) ◽  
pp. 419-426
Author(s):  
Yasunobu EGUCHI ◽  
Mitsuyuki SHIRAI ◽  
Kazuyoshi ARISHIMA ◽  
Masako YAMAMOTO ◽  
Yasuhiro UEDA
Keyword(s):  
Ultrastructural Changes ◽  
Follicular Cells ◽  
Fetal Rats ◽  
Thyroid Follicular Cells

Actions of plant cytokinins on dog thyroid follicular cells in vitro

1977 ◽  
Vol 26 (2) ◽  
pp. 251-259 ◽  
Author(s):  
J.A. Fernandez-Pol ◽  
M.T. Hays ◽  
J.P. Binette
Keyword(s):  
Follicular Cells ◽  
Thyroid Follicular Cells

scholarly journals Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering: a preliminary in vitro study

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Jie Weng ◽  
Bi Chen ◽  
Mengying Xie ◽  
Xinlong Wan ◽  
Peng Wang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
In Vitro Study ◽  
Biomechanical Properties ◽  
Space Structure ◽  
Thyroid Diseases ◽  
Human Thyroid ◽  
Thyroid Peroxidase ◽  
Follicular Cells ◽  
Thyroid Follicular Cells

Abstract Background Advances in regenerative medicine technologies have been strongly proposed in the management of thyroid diseases. Mechanistically, the adoption of thyroid bioengineering requires a scaffold that shares a similar three-dimensional (3D) space structure, biomechanical properties, protein component, and cytokines to the native extracellular matrix (ECM). Methods 24 male New Zealand white rabbits were used in this experimental study. The rabbit thyroid glands were decellularized by immersion/agitation decellularization protocol. The 3D thyroid decellularization scaffolds were tested with histological and immunostaining analyses, scanning electron microscopy, DNA quantification, mechanical properties test, cytokine assay and cytotoxicity assays. Meanwhile, the decellularization scaffold were seeded with human thyroid follicular cells, cell proliferation and thyroid peroxidase were determined to explore the biocompatibility in vitro. Results Notably, through the imaging studies, it was distinctly evident that our protocol intervention minimized cellular materials and maintained the 3D spatial structure, biomechanical properties, ECM composition, and biologic cytokine. Consequently, the decellularization scaffold was seeded with human thyroid follicular cells, thus strongly revealing its potential in reinforcing cell adhesion, proliferation, and preserve important protein expression. Conclusions The adoption of our protocol to generate a decellularized thyroid scaffold can potentially be utilized in transplantation to manage thyroid diseases through thyroid bioengineering.

scholarly journals Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering: a preliminary in vitro study

2021 ◽  
Author(s):  
Jie Weng ◽  
Bi Chen ◽  
Mengying Xie ◽  
Xinlong Wan ◽  
Wang Peng ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Biomechanical Properties ◽  
Space Structure ◽  
Thyroid Diseases ◽  
Human Thyroid ◽  
Thyroid Peroxidase ◽  
Follicular Cells ◽  
Thyroid Follicular Cells

Abstract Background: Advances in regenerative medicine technologies have been strongly proposed in the management of thyroid diseases. Mechanistically, the adoption of thyroid bioengineering requires a scaffold that shares a similar three dimensional (3D) space structure, biomechanical properties, protein component, and cytokines to the native extracellular matrix (ECM).Methods: 24 male New Zealand white rabbits were used in this experimental study. The rabbit thyroid glands were decellularized by immersion/agitation decellularization protocol. The 3D thyroid decellularization scaffolds were tested with histological and immunostaining analyses, scanning electron microscopy, DNA quantification, mechanical properties test, cytokine assay and cytotoxicity assays. Meanwhile, the decellularization scaffold were seeded with human thyroid follicular cells, cell proliferation and thyroid peroxidase were determined to explore the biocompatibility in vitro.Results: Notably, through the imaging studies, it was distinctly evident that our protocol intervention minimized cellular materials and maintained the 3D spatial structure, biomechanical properties, ECM composition, and biologic cytokine. Consequently, the decellularization scaffold was seeded with human thyroid follicular cells, thus strongly revealing its potential in reinforcing cell adhesion, proliferation, and preserve important protein expression.Conclusions: The adoption of our protocol to generate a decellularized thyroid scaffold can potentially be utilized in transplantation to manage thyroid diseases through thyroid bioengineering.

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