Induction of mitotic abnormalities in the root tips ofAllium cepa by tobacco smoke

1972 ◽  
Vol 28 (9) ◽  
pp. 1122-1123 ◽  
Author(s):  
P. R. Bhalla ◽  
T. S. Kochhar ◽  
P. S. Sabharwal
Keyword(s):  
Tobacco Smoke ◽  
Root Tips ◽  
Mitotic Abnormalities ◽  
Ofallium Cepa

scholarly journals Induction of Mitotic Abnormalities in Onion Root-Tips by Tobacco Smoke Condensate

CYTOLOGIA ◽  
1973 ◽  
Vol 38 (4) ◽  
pp. 707-712 ◽  
Author(s):  
P. R. Bhalla ◽  
T. S. Kochhar ◽  
P. S. Sabharwal
Keyword(s):  
Tobacco Smoke ◽  
Root Tips ◽  
Onion Root ◽  
Mitotic Abnormalities ◽  
Tobacco Smoke Condensate

Effect of trifluralin on the histology and respiration ofAllium cepa root tips

1978 ◽  
Vol 20 (1) ◽  
pp. 1-4 ◽  
Author(s):  
A. E. Dowidar ◽  
A. El-Nahas
Keyword(s):  
Root Tips ◽  
Ofallium Cepa

Effect of cannabis (hashish) on mitosis ofallium cepa L. root tips

1976 ◽  
Vol 18 (5) ◽  
pp. 401-407
Author(s):  
A. Kabaeity ◽  
A. El-Bayoumi ◽  
A. Habib
Keyword(s):  
Root Tips ◽  
Ofallium Cepa

scholarly journals Evaluation of Cytotoxic and Genotoxic Effect of Acacia nilotica (L.) Delile Extract Using Allium cepa Bioassay

2020 ◽  
pp. 154-159
Author(s):  
Samah Bodowara ◽  
Fauzia El Garaboli ◽  
Salem El shatshat
Keyword(s):  
Cell Division ◽  
Allium Cepa ◽  
Chromosomal Aberrations ◽  
Genetic Material ◽  
Genotoxic Effect ◽  
Bark Extract ◽  
Root Tips ◽  
Mitotic Abnormalities ◽  
Dividing Cells ◽  
Allium Cepa Assay

The present study aimed to measure the cytotoxic and genotoxic effect of the bark of A. nilotica extract. Allium cepa assay was used to find out the effect of A. nilotica extract on chromosome structure and behavior during cell division. The root tips meristem cells were treated with different concentration of A. nilotica bark aqueous extract (0.1, 0.01 and 0.001mg/ml) for 4, 6, 12 and 24 hours, respectively. Cytological analysis revealed decreasing in cell division in all used concentration especially at high ones. The obtained results indicate that aqueous extracts of A. nilotica plant have the ability to decrease the (MI%) values with increasing the concentration at (P<0.005). All treatments have caused different kind of mitotic abnormalities and chromosomal aberrations, such as: change percentage of mitotic phases, C-mitosis, stickiness, chromosome bridges, Micronucleus and vagrant chromosome. The action of A. nilotica bark extract on the genetic material led to decrease in dividing cells number which was concentration and time depended. This inhibition of cell division was due to disturbances in nucleus as a result of inhibition of DNA synthesis. KEY WORDS: Allium cepa assay: A. nilotica: Chromosomal aberrations; MI.

scholarly journals Systemic Control of Cell Division and Endoreduplication by NAA and BAP by Modulating CDKs in Root Tip Cells ofAllium cepa

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Jigna G. Tank ◽  
Vrinda S. Thaker
Keyword(s):  
Cell Division ◽  
Allium Cepa ◽  
Vegetative Growth ◽  
Research Work ◽  
Colchicine Treatment ◽  
Root Tip ◽  
Root Tips ◽  
Ofallium Cepa ◽  
Elongation Process ◽  
Growth Formation

Molecular mechanism regulated by auxin and cytokinin during endoreduplication, cell division, and elongation process is studied by usingAllium cepa rootsas a model system. The activity of CDK genes modulated by auxin and cytokinin during cell division, elongation, and endoreduplication process is explained in this research work. To study the significance of auxin and cytokinin in the management of cell division and endoreduplication process in plant meristematic cells at molecular level endoreduplication was developed in root tips ofAllium cepaby giving colchicine treatment. There were inhibition of vegetative growth, formation of c-tumor at root tip, and development of endoreduplicated cells after colchicine treatment. This c-tumor was further treated with NAA and BAP to reinitiate vegetative growth in roots. BAP gave positive response in reinitiation of vegetative growth of roots from center of c-tumor. However, NAA gave negative response in reinitiation of vegetative growth of roots from c-tumor. Further, CDKs gene expression analysis from normal, endoreduplicated, and phytohormone (NAA or BAP) treated root tip was done and remarkable changes in transcription level of CDK genes in normal, endoreduplicated, and phytohormones treated cells were observed.

Effect of morphine sulphate on mitosis ofAllium cepa L. root tips

1974 ◽  
Vol 16 (4) ◽  
pp. 275-282 ◽  
Author(s):  
A. Kabarity ◽  
A. El-Bayoumi ◽  
A. A. Habib
Keyword(s):  
Morphine Sulphate ◽  
Root Tips ◽  
Ofallium Cepa

Cytological effects of papaverine hydrochloride on root tips ofAllium cepa L.

1977 ◽  
Vol 19 (6) ◽  
pp. 472-476 ◽  
Author(s):  
A. S. El-Bayoumi ◽  
A. Kabarity ◽  
A. Habib
Keyword(s):  
Root Tips ◽  
Ofallium Cepa ◽  
Papaverine Hydrochloride

Effect of Niacin on Mitochondria of Allium Cepa Root Cells

1967 ◽  
Vol 25 ◽  
pp. 78-79
Author(s):  
M. Arif Hayat
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Hydrogen Transfer ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Nicotinamide Adenine ◽  
Root Tips ◽  
Root Cells ◽  
Transfer Processes ◽  
Standard Procedures ◽  
A Cell ◽  
Critical Interest

Although it is recognized that niacin (pyridine-3-carboxylic acid), incorporated as the amide in nicotinamide adenine dinucleotide (NAD) or in nicotinamide adenine dinucleotide phosphate (NADP), is a cofactor in hydrogen transfer in numerous enzyme reactions in all organisms studied, virtually no information is available on the effect of this vitamin on a cell at the submicroscopic level. Since mitochondria act as sites for many hydrogen transfer processes, the possible response of mitochondria to niacin treatment is, therefore, of critical interest.Onion bulbs were placed on vials filled with double distilled water in the dark at 25°C. After two days the bulbs and newly developed root system were transferred to vials containing 0.1% niacin. Root tips were collected at ¼, ½, 1, 2, 4, and 8 hr. intervals after treatment. The tissues were fixed in glutaraldehyde-OsO4 as well as in 2% KMnO4 according to standard procedures. In both cases, the tissues were dehydrated in an acetone series and embedded in Reynolds' lead citrate for 3-10 minutes.

Low temperature x-ray microanalysis of frozen-hydrated tobacco leaves

1984 ◽  
Vol 42 ◽  
pp. 284-285
Author(s):  
S. Edith Taylor ◽  
Patrick Echlin ◽  
May McKoon ◽  
Thomas L. Hayes
Keyword(s):  
Low Temperature ◽  
Lemna Minor ◽  
Root Tips ◽  
Tobacco Leaves ◽  
X Ray ◽  
Whole Cells ◽  
Carbon Coated ◽  
The Right ◽  
Beam Currents ◽  
The University

Low temperature x-ray microanalysis (LTXM) of solid biological materials has been documented for Lemna minor L. root tips. This discussion will be limited to a demonstration of LTXM for measuring relative elemental distributions of P,S,Cl and K species within whole cells of tobacco leaves.Mature Wisconsin-38 tobacco was grown in the greenhouse at the University of California, Berkeley and picked daily from the mid-stalk position (leaf #9). The tissue was excised from the right of the mid rib and rapidly frozen in liquid nitrogen slush. It was then placed into an Amray biochamber and maintained at 103K. Fracture faces of the tissue were prepared and carbon-coated in the biochamber. The prepared sample was transferred from the biochamber to the Amray 1000A SEM equipped with a cold stage to maintain low temperatures at 103K. Analyses were performed using a tungsten source with accelerating voltages of 17.5 to 20 KV and beam currents from 1-2nA.

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