Stopped-flow fluorescence studies of the interaction of a mutant form of cytochrome b5 with lipid vesicles

1994 ◽  
Vol 4 (3) ◽  
pp. 227-233 ◽  
Author(s):  
N. Krishnamachary ◽  
Frances A. Stephenson ◽  
Alan W. Steggles ◽  
Peter W. Holloway
Keyword(s):  
Cytochrome B5 ◽  
Lipid Vesicles ◽  
Stopped Flow ◽  
Mutant Form ◽  
Fluorescence Studies

scholarly journals Rat hepatic microsomal cytochrome b5. A simple large-scale purification procedure and antibody production by antigen-containing liposomes

1988 ◽  
Vol 256 (3) ◽  
pp. 1051-1054 ◽  
Author(s):  
J Carlsen ◽  
K Christiansen ◽  
H M Jensen
Keyword(s):  
Gel Electrophoresis ◽  
Large Scale ◽  
Polyacrylamide Gel Electrophoresis ◽  
Cytochrome B5 ◽  
Liver Microsomes ◽  
Lipid Vesicles ◽  
Rat Liver Microsomes ◽  
Native Form ◽  
Great Capacity ◽  
Microsomal Cytochrome B5

Cytochrome b5 from rat liver microsomes (microsomal fractions) was purified in its native form. The procedure described has great capacity, is fast, and the final product is pure as judged from SDS/polyacrylamide-gel electrophoresis. Antibodies to cytochrome b5 are obtained after administration of the antigen inserted into small unilamellar lipid vesicles.

Stopped-Flow Fluorescence Studies of HMG-Domain Protein Binding to Cisplatin-Modified DNA†

Biochemistry ◽  
2000 ◽  
Vol 39 (29) ◽  
pp. 8426-8438 ◽  
Author(s):  
Elizabeth R. Jamieson ◽  
Stephen J. Lippard
Keyword(s):  
Protein Binding ◽  
Stopped Flow ◽  
Fluorescence Studies ◽  
Modified Dna ◽  
Domain Protein

Stopped-flow fluorescence studies on binding of snake neurotoxins to acetylcholine receptor

1987 ◽  
Vol 6 (3) ◽  
Author(s):  
Toshiya Endo ◽  
Mamoru Nakanishi ◽  
Shoei Furukawa ◽  
FrancoisJ. Joubert ◽  
Nobuo Tamiya ◽  
...  
Keyword(s):  
Acetylcholine Receptor ◽  
Stopped Flow ◽  
Fluorescence Studies ◽  
Snake Neurotoxins

Determination of the topography of cytochrome b5 in lipid vesicles by fluorescence quenching

Biochemistry ◽  
1985 ◽  
Vol 24 (12) ◽  
pp. 2895-2901 ◽  
Author(s):  
Tom Markello ◽  
Adam Zlotnick ◽  
James Everett ◽  
Joan Tennyson ◽  
Peter W. Holloway
Keyword(s):  
Fluorescence Quenching ◽  
Cytochrome B5 ◽  
Lipid Vesicles

scholarly journals Investigation of the effect of metal ions on the reactivity of thiol groups in human 5-aminolaevulinate dehydratase

1985 ◽  
Vol 225 (3) ◽  
pp. 573-580 ◽  
Author(s):  
P N B Gibbs ◽  
M G Gore ◽  
P M Jordan
Keyword(s):  
Metal Ions ◽  
Binding Sites ◽  
The Other ◽  
Stopped Flow ◽  
Thiol Groups ◽  
Protein Fluorescence ◽  
Fluorescence Studies ◽  
Nitrobenzoic Acid ◽  
Rate Of Reaction ◽  
Molar Equivalent

The reaction of human 5-aminolaevulinate dehydratase with 5,5′-dithiobis-(2-nitrobenzoic acid) (Nbs2) results in the release of 4 molar equivalents of 5-mercapto-2-nitrobenzoic acid (Nbs) per subunit. Two of the thiol groups reacted very rapidly (groups I and II), and their rate constants were determined by stopped-flow spectrophotometry; the other two thiol groups (groups III and IV) were observed by conventional spectroscopy. Titration of the enzyme with a 1 molar equivalent concentration of Nbs2 resulted in the release of 2 molar equivalents of Nbs and the concomitant formation of an intramolecular disulphide bond between groups I and II. Removal of zinc from the holoenzyme increased the reactivity of groups I and II without significantly affecting the rate of reaction of the other groups. The reactions of the thiol groups in both the holoenzyme and apoenzyme were little affected by the presence of Pb2+ ions at concentrations that strongly inhibit the enzyme, suggesting that Zn2+ and Pb2+ ions may have independent binding sites. Protein fluorescence studies with Pb2+ and Zn2+ have shown that the binding of both metal ions results in perturbation of the protein fluorescence.

Analysis of the molecular requirements for T cell recognition and activation by using la-containing lipid vesicles and stopped-flow fluorometry

1990 ◽  
Vol 2 (6) ◽  
pp. 509-514 ◽  
Author(s):  
Chlkako Odaka ◽  
Naoko Utsunomiya ◽  
Mamoru Nakanishl ◽  
Yoji Arata ◽  
Sanae Manabe ◽  
...  
Keyword(s):  
T Cell ◽  
Lipid Vesicles ◽  
Stopped Flow ◽  
Cell Recognition ◽  
T Cell Recognition

scholarly journals Association of α-Synuclein with Lipid Vesicles. Stopped-Flow Kinetics of Concerted Binding and Conformational Change

2014 ◽  
Vol 106 (2) ◽  
pp. 248a
Author(s):  
Thomas M. Jovin ◽  
Volodymyr V. Shvadchak ◽  
Remco Siero ◽  
Lisandro J. Falomir-Lockhart ◽  
Vinod Subramaniam
Keyword(s):  
Conformational Change ◽  
Lipid Vesicles ◽  
Stopped Flow ◽  
Stopped Flow Kinetics ◽  
Kinetics Of
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