Determination of the topography of cytochrome b5 in lipid vesicles by fluorescence quenching

Biochemistry ◽  
1985 ◽  
Vol 24 (12) ◽  
pp. 2895-2901 ◽  
Author(s):  
Tom Markello ◽  
Adam Zlotnick ◽  
James Everett ◽  
Joan Tennyson ◽  
Peter W. Holloway
Keyword(s):  
Fluorescence Quenching ◽  
Cytochrome B5 ◽  
Lipid Vesicles

A Homogeneous Nonisotopic Histone Deacetylase Activity Assay

2003 ◽  
Vol 8 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Birgit Heltweg ◽  
Manfred Jung
Keyword(s):  
Drug Discovery ◽  
Fluorescence Quenching ◽  
Histone Deacetylase ◽  
Anticancer Agents ◽  
Histone Deacetylases ◽  
Activity Assay ◽  
Animal Testing ◽  
Hdac Activity

Histone deacetylases (HDACs) are important regulators of transcription, and their inhibitors are a promising class of anticancer agents. The methods for the determination of HDAC activity and its inhibition that are currently available suffer from various drawbacks, such as animal testing, radioactive substrates, or limited throughput. Therefore, a fast nonisotopic method for the measurement of HDAC activity is highly desirable. The authors present such an assay that relies on the fluorescent HDAC substrate developed previously in their group. After incubation of the substrate with the enzyme, a derivatization leads to efficient fluorescence quenching in the deacetylated metabolite. Thus, only the fluorescence emitted by the remaining substrate is detected, which allows for a convenient detection of HDAC activity in a homogeneous format that can be performed on multiwell plate readers. This procedure, called HDASH (histone deacetylase assay—homogeneous), should be a valuable tool in transcriptional research and especially drug discovery. ( Journal of Biomolecular Screening 2003:89-95)

Determination of Sodium Hexametaphosphate in Beverages Based on the Fluorescence Quenching of Acridine Orange

2014 ◽  
Vol 47 (16) ◽  
pp. 2740-2746
Author(s):  
Heng Xin Zhao ◽  
Ming Luo ◽  
Li Xin Mo ◽  
Liang Cheng ◽  
Zheng Ma ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Acridine Orange ◽  
Sodium Hexametaphosphate

An “on–off” fluorescent probe based on cucurbit[7]uril for highly sensitive determination of ammonia nitrogen in aquaculture water

2021 ◽  
Author(s):  
Zhen Li ◽  
Tan Wang ◽  
Xianbao Xu ◽  
Cong Wang ◽  
Daoliang Li
Keyword(s):  
Fluorescence Quenching ◽  
Inclusion Complex ◽  
Fluorescent Probe ◽  
Ammonia Nitrogen ◽  
Highly Sensitive ◽  
Sensitive Determination ◽  
Aquaculture Water

A novel “on–off” fluorescent probe for the determination of ammonia nitrogen has been synthesized. URO can replace PAL into the cavity of CB[7] to form a stable inclusion complex, eventually forming the fluorescence quenching system of URO@CB[7].

Determination of lateral diffusion coefficients in air-water monolayers by fluorescence quenching measurements

1991 ◽  
Vol 113 (13) ◽  
pp. 4838-4843 ◽  
Author(s):  
F. Caruso ◽  
F. Grieser ◽  
A. Murphy ◽  
P. Thistlethwaite ◽  
R. Urquhart ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Diffusion Coefficients ◽  
Lateral Diffusion
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