Pertussis toxin stimulation of catecholamine release from adrenal medullary chromaffin cells: Mechanism may be by direct activation of L-type and G-type calcium channels

1991 ◽  
Vol 122 (1) ◽  
pp. 23-31 ◽  
Author(s):  
V. Ceña ◽  
K. W. Brocklehurst ◽  
H. B. Pollard ◽  
E. Rojas
Keyword(s):  
Calcium Channels ◽  
Pertussis Toxin ◽  
Chromaffin Cells ◽  
Catecholamine Release ◽  
Direct Activation ◽  
Stimulation Of

scholarly journals A pertussis-toxin-sensitive protein controls exocytosis in chromaffin cells at a step distal to the generation of second messengers

1991 ◽  
Vol 274 (2) ◽  
pp. 339-347 ◽  
Author(s):  
J M Sontag ◽  
D Thierse ◽  
B Rouot ◽  
D Aunis ◽  
M F Bader
Keyword(s):  
Arachidonic Acid ◽  
Cyclic Amp ◽  
G Protein ◽  
Pertussis Toxin ◽  
Chromaffin Cells ◽  
Second Messengers ◽  
Catecholamine Release ◽  
Secretory Process ◽  
Permeabilized Cells ◽  
Arachidonic Acid Concentration

The role of GTP-binding proteins (G-proteins) in the secretory process in chromaffin cells was investigated by studying the effects of pertussis toxin (PTX) on catecholamine release and generation of various second messengers. PTX was found to stimulate the catecholamine secretion induced by nicotine, 59 mM-K+ or veratridine. PTX also potentiated Ca2(+)-evoked catecholamine release from permeabilized chromaffin cells, suggesting that PTX substrate(s) regulate the exocytotic machinery at a step distal to the rise in intracellular Ca2+. We have investigated the possible intracellular pathways involved in the stimulation of secretion by PTX. PTX did not modify the translocation of protein kinase C (PKC) to membranes in intact or permeabilized cells; in addition, neither inhibitors nor activators of PKC had any effect on catecholamine release induced by PTX. Thus it seems unlikely that the effect of PTX on secretion is mediated by activation of PKC. The effect of PTX is also cyclic AMP-independent, as PTX did not change cytoplasmic cyclic AMP levels. The relationship between PTX treatment and arachidonic acid release was also examined. We found that an increase in cytoplasmic arachidonic acid concentration enhanced Ca2(+)-evoked catecholamine release in permeabilized cells, but arachidonic acid did not mimic the effect of PTX on the Ca2(+)-dose-response curve for secretion. Furthermore, PTX did not significantly modify the release of arachidonic acid measured in resting or stimulated chromaffin cells, suggesting that the stimulatory effect of PTX on secretion is not mediated by an activation of phospholipase A2. Taken together, these results suggest that PTX may modulate the intracellular machinery of secretion at a step distal to the generation of second messengers. In alpha-toxin-permeabilized cells, full retention of the PTX-induced activation of secretion was observed even 30 min after permeabilization. In contrast, when chromaffin cells were permeabilized with streptolysin-O (SLO), there was a marked progressive loss of the PTX effect. We found that SLO caused the rapid leakage of three G-protein alpha-subunits which are specifically ADP-ribosylated by PTX. We propose that a PTX-sensitive G-protein may play an inhibitory role in the final stages of the Ca2(+)-evoked secretory process in chromaffin cells.

Pertussis toxin facilitates secretagogue-induced catecholamine release from cultured bovine adrenal chromaffin cells

1987 ◽  
Vol 144 (2) ◽  
pp. 907-914 ◽  
Author(s):  
Teruo Tanaka ◽  
Hiromitsu Yokohama ◽  
Manabu Negishi ◽  
Hideya Hayashi ◽  
Seiji Ito ◽  
...  
Keyword(s):  
Pertussis Toxin ◽  
Chromaffin Cells ◽  
Catecholamine Release ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin

scholarly journals Histaminic stimulation of catecholamine release and calcium influx in cultured bovine adrenal chromaffin cells

1989 ◽  
Vol 49 ◽  
pp. 186
Author(s):  
Yasuko Ishimura ◽  
Atsushi Nakanishi ◽  
Masanori Yoshizumi ◽  
Yoshihiro Murakumo ◽  
Kyoji Morita ◽  
...  
Keyword(s):  
Chromaffin Cells ◽  
Calcium Influx ◽  
Catecholamine Release ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin ◽  
Stimulation Of

scholarly journals Controlled on-chip stimulation of quantal catecholamine release from chromaffin cells using photolysis of caged Ca2+on transparent indium-tin-oxide microchip electrodes

Lab on a Chip ◽  
2008 ◽  
Vol 8 (1) ◽  
pp. 161-169 ◽  
Author(s):  
Xiaohui Chen ◽  
Yuanfang Gao ◽  
Maruf Hossain ◽  
Shubhra Gangopadhyay ◽  
Kevin D. Gillis
Keyword(s):  
Tin Oxide ◽  
Indium Tin Oxide ◽  
Chromaffin Cells ◽  
Catecholamine Release ◽  
On Chip ◽  
Stimulation Of
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