A kinetic analysis of the electrogenic pump ofChara corallina: III. Pump activity during action potential

1985 ◽  
Vol 86 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Uichiro Kishimoto ◽  
Yuko Takeuchi ◽  
Taka-aki Ohkawa ◽  
Nobunori Kami-ike
Keyword(s):  
Action Potential ◽  
Kinetic Analysis ◽  
Electrogenic Pump ◽  
Pump Activity

A kinetic analysis of the electrogenic pump ofChara corallina: IV. Temperature dependence of the pump activity

1986 ◽  
Vol 94 (2) ◽  
pp. 163-171 ◽  
Author(s):  
Nobunori Kami-ike ◽  
Taka-aki Ohkawa ◽  
Uichiro Kishimoto ◽  
Yuko Takeuchi
Keyword(s):  
Temperature Dependence ◽  
Kinetic Analysis ◽  
Electrogenic Pump ◽  
Pump Activity

A kinetic analysis of the electrogenic pump ofChara corallina: I. Inhibition of the pump by DCCD

1984 ◽  
Vol 80 (2) ◽  
pp. 175-183 ◽  
Author(s):  
U. Kishimoto ◽  
N. Kami-ike ◽  
Y. Takeuchi ◽  
T. Ohkawa
Keyword(s):  
Kinetic Analysis ◽  
Electrogenic Pump

Ligand and voltage gated sodium channels may regulate electrogenic pump activity in human, mouse and rat lymphocytes

1989 ◽  
Vol 160 (3) ◽  
pp. 999-1002 ◽  
Author(s):  
Carlo Pieri ◽  
Rina Recchioni ◽  
Fausto Moroni ◽  
László Balkay ◽  
Teréz Márián ◽  
...  
Keyword(s):  
Sodium Channels ◽  
Electrogenic Pump ◽  
Voltage Gated ◽  
Voltage Gated Sodium Channels ◽  
Rat Lymphocytes ◽  
Pump Activity

How to sample the entire length of a single muscle fiber quick-frozen after electrical point stimulation for high resolution EM

1992 ◽  
Vol 50 (1) ◽  
pp. 776-777
Author(s):  
Joachim R. Sommer ◽  
Teresa High ◽  
Betty Scherer ◽  
Isaiah Taylor ◽  
Rashid Nassar
Keyword(s):  
Skeletal Muscle ◽  
High Resolution ◽  
Action Potential ◽  
Muscle Fiber ◽  
Freeze Fracture ◽  
Freeze Substitution ◽  
Electrical Field Stimulation ◽  
Skeletal Muscle Fiber ◽  
Freeze Dried ◽  
Quick Freezing

We have developed a model that allows the quick-freezing at known time intervals following electrical field stimulation of a single, intact frog skeletal muscle fiber isolated by sharp dissection. The preparation is used for studying high resolution morphology by freeze-substitution and freeze-fracture and for electron probe x-ray microanlysis of sudden calcium displacement from intracellular stores in freeze-dried cryosections, all in the same fiber. We now show the feasibility and instrumentation of new methodology for stimulating a single, intact skeletal muscle fiber at a point resulting in the propagation of an action potential, followed by quick-freezing with sub-millisecond temporal resolution after electrical stimulation, followed by multiple sampling of the frozen muscle fiber for freeze-substitution, freeze-fracture (not shown) and cryosectionmg. This model, at once serving as its own control and obviating consideration of variances between different fibers, frogs etc., is useful to investigate structural and topochemical alterations occurring in the wake of an action potential.

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