Amiloride-sensitive Na+ transport in human red cells: Evidence for a Na/H exchange system

1986 ◽  
Vol 90 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Nelson Escobales ◽  
Mitzy Canessa
Keyword(s):  
Red Cells ◽  
Na Transport ◽  
Exchange System ◽  
Human Red Cells

Effects of General Anaesthetics on Na+ Transport in Human Red Cells

Nature ◽  
1970 ◽  
Vol 225 (5238) ◽  
pp. 1151-1152 ◽  
Author(s):  
M. J. HALSEY ◽  
E. B. SMITH ◽  
T. E. WOOD
Keyword(s):  
Red Cells ◽  
Na Transport ◽  
Human Red Cells

scholarly journals Further Studies of Sodium Transport in Feline Red Cells

1973 ◽  
Vol 61 (6) ◽  
pp. 709-726 ◽  
Author(s):  
R. I. Sha'afi ◽  
E. Pascoe
Keyword(s):  
Blood Cells ◽  
Red Cells ◽  
Na Transport ◽  
Experimental Conditions ◽  
High Sodium ◽  
Order Of Magnitude ◽  
Radioactive Sodium ◽  
Lag Period ◽  
Magnitude Increase ◽  
Human Red Cells

The transport of radioactive sodium in high sodium cat red blood cells has been studied under various experimental conditions. It was found that iodoacetate (IAA) and iodoacetamide (IAM) inhibit Na influx by 50% whereas NaF has no effect. Reversible dyes, such as methylene blue (Mb), also inhibit this influx by 60%. Both IAA and Mb effects show a lag period of about 40 min. Cell starvation abolishes the volume-dependent Na influx which is generally observed in these cells. IAA reduces significantly the volume-dependent Na influx but does not inhibit it completely. 5 mM magnesium chloride produces a twofold increase in Na influx. On the other hand, MgCl2 has no effect on Na transport in human red cells or on potassium or sulfate transport in cat red cells. The effect of MgCl2 is quite rapid and does not interfere with the volume-dependent Na influx. This effect is abolished in starved cells. Reincubation of previously stored cells in buffered solutions containing glucose and MgCl2 causes more than one order of magnitude increase in Na influx. These several observations are discussed in terms of the possibility of a link between Na transport and Na-Mg-activated ATPase.

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

Metabolic control of the K+ channel of human red cells

1990 ◽  
Vol 116 (1) ◽  
pp. 19-29 ◽  
Author(s):  
Pedro J. Romero ◽  
Carlos E. Ortíz ◽  
Carmelo Melitto
Keyword(s):  
Metabolic Control ◽  
Red Cells ◽  
K Channel ◽  
Human Red Cells

Interactions between sphered human red cells in tube flow: Technique for measuring the strength of antigen-antibody bonds

1982 ◽  
Vol 23 (2) ◽  
pp. 231-238 ◽  
Author(s):  
Harry L. Goldsmith ◽  
Phil Gold ◽  
Joseph Shuster ◽  
Koichi Takamura
Keyword(s):  
Red Cells ◽  
Tube Flow ◽  
Antigen Antibody ◽  
Human Red Cells

The distribution of intracellular calcium chelator (fura-2) in a population of intact human red cells

1993 ◽  
Vol 1148 (1) ◽  
pp. 152-156 ◽  
Author(s):  
Virgilio L. Lew ◽  
Zipora Etzion ◽  
Robert M. Bookchin ◽  
Rui daCosta ◽  
Heikki Väänänen ◽  
...  
Keyword(s):  
Intracellular Calcium ◽  
Red Cells ◽  
Fura 2 ◽  
Human Red Cells

scholarly journals HUMAN T CELLS BIND AUTOLOGOUS AND HOMOLOGOUS HUMAN RED CELLS

1974 ◽  
Vol 8 (4) ◽  
pp. 402-402
Author(s):  
Joseph Kaplan ◽  
C S Stulberg
Keyword(s):  
T Cells ◽  
Red Cells ◽  
Human T Cells ◽  
Human Red Cells

Caffeine activates a mechanosensitive Ca2+ channel in human red cells

Cell Calcium ◽  
2002 ◽  
Vol 31 (5) ◽  
pp. 189-200 ◽  
Author(s):  
J.F Cordero ◽  
P.J Romero
Keyword(s):  
Red Cells ◽  
Human Red Cells
Sign in / Sign up

Export Citation Format

Share Document