The electrophoretic mobility test with myelin basic protein, binding of125I-MBP to lymphocytes, and gel electrophoresis pattern of supernatant during incubation

1980 ◽  
Vol 177 (3) ◽  
pp. 245-253
Author(s):  
K. Mross ◽  
B. Mross ◽  
D. I. Wolfrum ◽  
A. Kohlschütter
Keyword(s):  
Protein Binding ◽  
Myelin Basic Protein ◽  
Electrophoretic Mobility ◽  
Gel Electrophoresis ◽  
Basic Protein ◽  
Mobility Test ◽  
Electrophoretic Mobility Test ◽  
Electrophoresis Pattern

scholarly journals An electrophoretic method for selection of conditions for production of electrophoretically uniform protein colloidal gold complexes.

1991 ◽  
Vol 39 (1) ◽  
pp. 111-121 ◽  
Author(s):  
W D Geoghegan
Keyword(s):  
Electrophoretic Mobility ◽  
Colloidal Gold ◽  
Gel Electrophoresis ◽  
Agarose Gel Electrophoresis ◽  
Gold Complexes ◽  
Electrophoretic Method ◽  
Mobility Test ◽  
Classical Procedure ◽  
Electrophoretic Mobility Test ◽  
Selection Of

An electrophoretic method was developed to determine the conditions required for production of electrophoretically uniform protein-colloidal gold complexes from monodisperse colloidal gold (Au) and electrophoretically uniform protein. The method is based on the electrophoretic migration of protein-Au complexes in agarose. The results demonstrate that two variables, the pH of adsorption and the quantity of protein added, can be manipulated to vary the electrophoretic mobility of the resulting protein-Au complexes. Thus, agarose gel electrophoresis can be used to select the pH of adsorption and the quantity of protein required to produce electrophoretically uniform protein-Au complexes. This new electrophoretic mobility test can be used in place of or in addition to the classical procedure of Zsigmondy and its many variations, both visual and spectrophotometric. The procedure described is also useful for electrophoretic comparison of small quantities of various protein-Au samples.

SIMPLIFIED RED-CELL ELECTROPHORETIC MOBILITY TEST FOR MULTIPLE SCLEROSIS

The Lancet ◽  
1979 ◽  
Vol 313 (8126) ◽  
pp. 1138-1139 ◽  
Author(s):  
GeoffreyV.F. Seaman ◽  
RoyL. Swank ◽  
CherryH. Tamblyn ◽  
CharlesF. Zukoski
Keyword(s):  
Multiple Sclerosis ◽  
Electrophoretic Mobility ◽  
Red Cell ◽  
Mobility Test ◽  
Electrophoretic Mobility Test

Two-dimensional gel electrophoresis of human brain proteins. V. Non-equilibrium gel electrophoresis, with detection of a myelin basic protein mutation--MBL-Duarte.

1982 ◽  
Vol 28 (4) ◽  
pp. 813-818 ◽  
Author(s):  
D E Comings ◽  
A Pekkula-Flagan
Keyword(s):  
Human Brain ◽  
Myelin Basic Protein ◽  
Gel Electrophoresis ◽  
Basic Protein ◽  
Myelin Proteins ◽  
Two Dimensional Gel Electrophoresis ◽  
Brain Proteins ◽  
Basic Proteins ◽  
A Charge ◽  
Non Equilibrium

Abstract To examine the basic human brain proteins, we subjected 9 mmol/L urea extracts to non-equilibrium gel electrophoresis. The pattern observed differs distinctly from that with equilibrium gel electrophoresis. With this technique, the myelin proteins (myelin basic protein, proteolipids, and basic Wolfgram proteins) and many other unindentified major basic proteins can be demonstrated. The myelin basic proteins occur as two major polypeptides of different charge and slightly different molecular mass, indicating the action of at least two genes. The proteolipid proteins occur as a long series of charge isomers, suggesting multiple genes or extensive post-transcriptional modification. In one patient with schizophrenia, a charge-change mutation of the larger myelin basic protein (MBL) was observed and is termed "MBL-Duarte."

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