Effects of pantothenic acid on fibroblastic cell cultures

1988 ◽  
Vol 188 (5) ◽  
pp. 391-396 ◽  
Author(s):  
B. Lacroix ◽  
E. Didier ◽  
J. F. Grenier
Keyword(s):  
Cell Cultures ◽  
Pantothenic Acid ◽  
Fibroblastic Cell

Comparative use of fructose and glucose in human liver and fibroblastic cell cultures

In Vitro ◽  
1984 ◽  
Vol 20 (9) ◽  
pp. 699-706 ◽  
Author(s):  
B. Delhotal ◽  
F. Lemonnier ◽  
M. Couturier ◽  
C. Wolfrom ◽  
M. Gautier ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Human Liver ◽  
Fibroblastic Cell

The production and effect of interferon on influenza virus growth in chick embryo lung epithelial and fibroblast cell cultures

1969 ◽  
Vol 15 (3) ◽  
pp. 273-277 ◽  
Author(s):  
Sunidhkumar S. Gandhi ◽  
Robert B. Stewart
Keyword(s):  
Epithelial Cells ◽  
Chick Embryo ◽  
Cell Cultures ◽  
Fibroblast Cell ◽  
Cell Types ◽  
Fibroblastic Cell ◽  
Virus Growth ◽  
Lung Epithelial ◽  
Fibroblastic Cells ◽  
Virus Strains

Cultures of fibroblastic cells prepared from chick embryo lung infected with low multiplicities of influenza type A virus strains were found to produce more interferon than did cultures of epithelial cells prepared from the same organ. Fibroblastic cell cultures were also found to be more sensitive to the action of interferon than were epithelial cells with respect to the levels of infectious virus produced and the duration of interferon action. Cultures of the two cell types treated with interferon did not differ with respect to the number of cells involved in virus synthesis.

Inductive Properties of Fibroblastic Cell Cultures Derived from Rat Intestinal Mucosa on Epithelial Differentiation*

1982 ◽  
Vol 23 (1-3) ◽  
pp. 226-233 ◽  
Author(s):  
Katy Haffen ◽  
Brigitte Lacroix ◽  
Michèle Kedinger ◽  
Patricia M. Simon-Assmann
Keyword(s):  
Cell Cultures ◽  
Intestinal Mucosa ◽  
Epithelial Differentiation ◽  
Fibroblastic Cell

Glycosaminoglycan expression in intestinal epithelial skin-fibroblastic cell cocultures. Fibroblastic cell-mediated effects of glucocorticoids

1989 ◽  
Vol 92 (4) ◽  
pp. 679-685
Author(s):  
F. Bouziges ◽  
P. Simon-Assmann ◽  
C. Leberquier ◽  
K. Haffen ◽  
M. Kedinger
Keyword(s):  
Molecular Weight ◽  
Cell Surface ◽  
Cell Cultures ◽  
Chondroitin Sulphate ◽  
Heparan Sulphate ◽  
Cell Types ◽  
Molecular Forms ◽  
Fibroblastic Cell ◽  
Endodermal Cells ◽  
Molecular Weight Form

The nature and distribution of newly synthesized glycosaminoglycans (GAGs) were studied in foetal rat skin fibroblasts, in rat intestinal endodermal cells and in cocultures of both cell types. The data show that fibroblasts synthesize and secrete hyaluronic acid (HA), heparan sulphate (HS) and chondroitin sulphate molecules (CS). Our data focus on HA, which is found as two different molecular forms, the smallest hydrodynamic-sized species being mostly recovered within the cell or associated with the cell surface, and the largest one secreted into the medium, whatever the cell type. Endodermal cells synthesize only two types of GAGs: the low molecular weight form of HA and HS. Cocultures of rat intestinal endodermal and skin fibroblastic cells in the presence of dexamethasone (Dx), allow optimal epithelial cytodifferentiation (Kedinger et al. 1987a). The main changes in the GAGs synthesized under these conditions as compared to skin fibroblastic cell cultures concern: (1) the enhancement of the lowest molecular weight form of HA to the detriment of the highest form in the cellular, pericellular and extracellular compartments; (2) the increase in the proportion of HS molecules associated with the cell surface. Interestingly, similar modifications are obtained by addition of Dx to the skin fibroblastic cell cultures. The data are discussed with reference to the constitution of a basement membrane at the epithelial-fibroblast interface in the cocultures, to the fibroblastic-dependent induction of epithelial differentiation and to the glucocorticoid response.

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

Bacteriophages Associated with Turkey Coecums in Bluecomb-Infected and Healthy Birds

1969 ◽  
Vol 27 ◽  
pp. 226-227
Author(s):  
A. E. Ritchie
Keyword(s):  
Host Cell ◽  
Causal Relationship ◽  
Cell Cultures ◽  
Cell Interaction ◽  
Etiologic Agent ◽  
Viral Origin ◽  
Intestinal Contents ◽  
Host Cell Interaction

The cause of bluecomb disease in turkeys is unknown. Filtration of infective intestinal contents suggests a viral origin. To date, it has not been possible to isolate the etiologic agent in various cell cultures. The purpose of this work was to characterize as many virus-like entities as were recognizable in intestines of both healthy and bluecomb-infected turkeys. By a comparison of the viral populations it was hoped that some insight might be gained into the cause of this disease. Studies of turkey hemorraghic enteritis by Gross and Moore (Avian Dis. 11: 296-307, 1967) have suggested that a bacteriophage-host cell interaction may bear some causal relationship to that disease.

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