Reusable tissue culture growth chambers

1983 ◽  
Vol 8 (3) ◽  
pp. 95-96 ◽  
Author(s):  
Robert J. Rudd ◽  
Morris Safford
Keyword(s):  
Tissue Culture ◽  
Growth Chambers ◽  
Culture Growth

Growth and metabolism of Ricinus communis endosperm in tissue culture

1970 ◽  
Vol 48 (12) ◽  
pp. 2323-2331 ◽  
Author(s):  
D. J. Brown ◽  
D. T. Canvin ◽  
B. F. Zilkey
Keyword(s):  
Tissue Culture ◽  
Ricinus Communis ◽  
Glyoxylate Cycle ◽  
Culture Conditions ◽  
Endosperm Tissue ◽  
Ricinus Communis L ◽  
Culture Growth ◽  
Germinating Seeds

Endosperm tissue from both developing and germinating castor oil seeds (Ricinus communis L.) was grown as a callus in tissue culture. Callus growths were established from endosperm explants (without embryo) at all stages of seed development except from the quiescent unimbibed seed. Best tissue culture growth was observed with endosperm tissue obtained from seeds that had been germinated for 2 days.The lipid reserves diminished in all endosperm tissue which had been established on culture from germinating seeds but the rate of breakdown was much slower in cultures from 0-day germinated seeds. Glyoxylate cycle activity, as tested by acetate-1- and -2-14C incorporation, was not evident in long-term cultures.The process of lipid accumulation found in normal developing endosperm (24 to 36 days after fertilization) was not retained in culture but was replaced by a process resulting in the rapid loss of the already accumulated oil reserves.As far as could be determined the in vivo biochemical capabilities of the castor bean endosperm were not retained for a lengthy period in the tissue culture conditions used.

Studies in Tissue Culture Growth of Laryngeal Papilloma

1957 ◽  
Vol 48 (1-2) ◽  
pp. 14-15 ◽  
Author(s):  
Robert A. West ◽  
Joseph D. Boggs ◽  
Paul H. Holinger
Keyword(s):  
Tissue Culture ◽  
Laryngeal Papilloma ◽  
Culture Growth

Aspect morphologique et composition chimique de Skeletonema costatum(Bacillariophyceae) croissant en milieu nutritif naturel à l'aide d'un système de culture à fibres dialysantes

1983 ◽  
Vol 29 (10) ◽  
pp. 1235-1240 ◽  
Author(s):  
Pierre Marsot ◽  
Marc Leclerc ◽  
Réal Fournier
Keyword(s):  
Normal Development ◽  
Skeletonema Costatum ◽  
Cell Number ◽  
Growth Stages ◽  
Biological Productivity ◽  
Composition Chimique ◽  
Average Cell ◽  
Growth Chambers ◽  
Culture Growth ◽  
Different Growth Stages

The growth of Skeletonema costatum, under natural nutriment conditions, was studied using a bulk culture fiber dialyzing apparatus. The diatom displayed normal development of chain length (average cell number per chain) which coincided with the culture growth stages; that is, the cell number per colony increased during the active division period and decreased thereafter with the beginning of the prestationary phase. This morphological behaviour showed that the alga cells were not affected by such physical shocks as collision or tension occurring during repeated cell transfers from growth chambers to the dialyzing apparatus or at the time of their passage through the fiber fascicles. Measured at different growth stages, the cell contents in carbon, nitrogen, and chlorophyll confirmed the above results and showed for S. costatum a biological productivity comparable with that obtained in smaller dialyzing containers (dialyzing bags). Through a comparison between the dialyzing culture and a static culture grown in an enriched medium, certain characteristics were determined.[Translated by the journal]

Predicting in vitro tissue culture growth for cytogenetic evaluation of stillborn fetuses

1985 ◽  
Vol 19 (3) ◽  
pp. 167-174 ◽  
Author(s):  
Trevor A. Macpherson ◽  
Kenneth L. Garver ◽  
J.Howard Turner ◽  
Gerard R. Diggans ◽  
Sandra G. Marchese ◽  
...  
Keyword(s):  
Tissue Culture ◽  
In Vitro Tissue Culture ◽  
Culture Growth

Mammalian Tissue Culture Growth, Viral Replication, and Cultivation Using Serum Replacement Factor

1989 ◽  
Vol 192 (1) ◽  
pp. 1-5 ◽  
Author(s):  
F. J. Candal ◽  
G. Marchetti ◽  
M. Davis ◽  
V. George ◽  
E. W. Ades
Keyword(s):  
Tissue Culture ◽  
Viral Replication ◽  
Mammalian Tissue ◽  
Serum Replacement ◽  
Culture Growth ◽  
Replacement Factor

Artificial modification of the viral regulatory region improves tissue culture growth of SV40 strain 776

1995 ◽  
Vol 35 (2) ◽  
pp. 143-153 ◽  
Author(s):  
John A. Lednicky ◽  
Connie Wong ◽  
Janet S. Butel
Keyword(s):  
Tissue Culture ◽  
Regulatory Region ◽  
Culture Growth
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