Aspect morphologique et composition chimique de Skeletonema costatum(Bacillariophyceae) croissant en milieu nutritif naturel à l'aide d'un système de culture à fibres dialysantes

1983 ◽  
Vol 29 (10) ◽  
pp. 1235-1240 ◽  
Author(s):  
Pierre Marsot ◽  
Marc Leclerc ◽  
Réal Fournier

The growth of Skeletonema costatum, under natural nutriment conditions, was studied using a bulk culture fiber dialyzing apparatus. The diatom displayed normal development of chain length (average cell number per chain) which coincided with the culture growth stages; that is, the cell number per colony increased during the active division period and decreased thereafter with the beginning of the prestationary phase. This morphological behaviour showed that the alga cells were not affected by such physical shocks as collision or tension occurring during repeated cell transfers from growth chambers to the dialyzing apparatus or at the time of their passage through the fiber fascicles. Measured at different growth stages, the cell contents in carbon, nitrogen, and chlorophyll confirmed the above results and showed for S. costatum a biological productivity comparable with that obtained in smaller dialyzing containers (dialyzing bags). Through a comparison between the dialyzing culture and a static culture grown in an enriched medium, certain characteristics were determined.[Translated by the journal]

1997 ◽  
Vol 99 (1) ◽  
pp. 185-189
Author(s):  
Wen-Shaw Chen ◽  
Kuang-Liang Huang ◽  
Hsiao-Ching Yu

2013 ◽  
Vol 39 (5) ◽  
pp. 919 ◽  
Author(s):  
Bo MING ◽  
Jin-Cheng ZHU ◽  
Hong-Bin TAO ◽  
Li-Na XU ◽  
Bu-Qing GUO ◽  
...  

GigaScience ◽  
2021 ◽  
Vol 10 (5) ◽  
Author(s):  
Teng Miao ◽  
Weiliang Wen ◽  
Yinglun Li ◽  
Sheng Wu ◽  
Chao Zhu ◽  
...  

Abstract Background The 3D point cloud is the most direct and effective data form for studying plant structure and morphology. In point cloud studies, the point cloud segmentation of individual plants to organs directly determines the accuracy of organ-level phenotype estimation and the reliability of the 3D plant reconstruction. However, highly accurate, automatic, and robust point cloud segmentation approaches for plants are unavailable. Thus, the high-throughput segmentation of many shoots is challenging. Although deep learning can feasibly solve this issue, software tools for 3D point cloud annotation to construct the training dataset are lacking. Results We propose a top-to-down point cloud segmentation algorithm using optimal transportation distance for maize shoots. We apply our point cloud annotation toolkit for maize shoots, Label3DMaize, to achieve semi-automatic point cloud segmentation and annotation of maize shoots at different growth stages, through a series of operations, including stem segmentation, coarse segmentation, fine segmentation, and sample-based segmentation. The toolkit takes ∼4–10 minutes to segment a maize shoot and consumes 10–20% of the total time if only coarse segmentation is required. Fine segmentation is more detailed than coarse segmentation, especially at the organ connection regions. The accuracy of coarse segmentation can reach 97.2% that of fine segmentation. Conclusion Label3DMaize integrates point cloud segmentation algorithms and manual interactive operations, realizing semi-automatic point cloud segmentation of maize shoots at different growth stages. The toolkit provides a practical data annotation tool for further online segmentation research based on deep learning and is expected to promote automatic point cloud processing of various plants.


2021 ◽  
Author(s):  
Xianhong Huang ◽  
Zhixin Wang ◽  
Jianliang Huang ◽  
Shaobing Peng ◽  
Dongliang Xiong

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