Toxicological detection of pholcodine and its metabolites in urine and hair using radio immunoassay, fluorescence polarisation immunoassay, enzyme immunoassay and gas chromatography-mass spectrometry

1990 ◽  
Vol 104 (1) ◽  
pp. 43-46 ◽  
Author(s):  
Hans H. Maurer ◽  
Christian F. Fritz
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Enzyme Immunoassay ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Radio Immunoassay ◽  
Fluorescence Polarisation

scholarly journals Identification of 19-nor-deoxycorticosterone in normal rat serum by enzyme immunoassay and gas chromatography-mass spectrometry.

1984 ◽  
Vol 31 (1) ◽  
pp. 49-53 ◽  
Author(s):  
YOSHIHARU KOBAYASHI ◽  
TOSHIO OGIHARA ◽  
YAYOI YAMAMURA ◽  
FUKUKO WATANABE ◽  
TOSHIKO KIGUCHI ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Enzyme Immunoassay ◽  
Gas Chromatography Mass Spectrometry ◽  
Rat Serum ◽  
Chromatography Mass Spectrometry ◽  
Normal Rat

Rapid, direct enzyme immunoassay of 11-keto-thromboxane B2 in urine, validated by immunoaffinity/gas chromatography-mass spectrometry

1993 ◽  
Vol 39 (12) ◽  
pp. 2470-2477 ◽  
Author(s):  
R Djurup ◽  
C Chiabrando ◽  
A Jörres ◽  
R Fanelli ◽  
M Foegh ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Enzyme Immunoassay ◽  
Cross Reactivity ◽  
Thromboxane B2 ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Measured Concentration ◽  
Chromatography Mass Spectrometry ◽  
Urine Specimens

Abstract We have developed a direct enzyme immunoassay (EIA) for quantifying immunoreactive 11-keto-thromboxane B2 (iKTXB) in unprocessed human urine. Cross-reactivity with other thromboxane metabolites and prostanoids was negligible. Analytical recovery of 11-keto-TXB2 in urine specimens was 97.4% to 99.8%. Total imprecision for two clinical specimens was 8.5% and 12.2%. Intake of acetylsalicylic acid decreased the measured concentration of iKTXB. Cardiopulmonary bypass, a procedure known to activate platelets, increased the mean excretion rate of iKTXB 10-fold. Simultaneous gas chromatography-mass spectrometry analysis of 11-keto-TXB2 and 11-keto-2,3-dinor TXB2 in urine specimens (n = 17) from healthy subjects indicated that urinary iKTXB concentrations measured by EIA represented a sum of the two 11-keto metabolites. We conclude that the direct EIA is sufficiently sensitive, rapid, simple, and specific to allow screening for alterations in thromboxane biosynthesis in patients.

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