A Comparison of Enzyme Immunoassay and Gas Chromatography/Mass Spectrometry in Forensic Toxicology

1980 ◽  
Vol 25 (2) ◽  
pp. 12128J ◽  
Author(s):  
John Fenton ◽  
Michael Schaffer ◽  
Nancy Wu Chen ◽  
E. W. Bermes
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Enzyme Immunoassay ◽  
Forensic Toxicology ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Benzodiazepines identified by capillary gas chromatography-mass spectrometry, with specific ion screening used to detect benzophenone derivatives.

1989 ◽  
Vol 35 (7) ◽  
pp. 1394-1398 ◽  
Author(s):  
C E Jones ◽  
F H Wians ◽  
L A Martinez ◽  
G J Merritt
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Forensic Toxicology ◽  
Urine Samples ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Selection Mode ◽  
Unequivocal Identification ◽  
Hydrolysis Of ◽  
Derivatives Of

Abstract We developed algorithms for confirmation and identification of benzodiazepines and their metabolites, initially detected in urine samples by enzyme-multiplied immunoassay (EMIT). These algorithms are based on the pattern of benzophenone derivatives of benzodiazepines obtained by gas chromatography-mass spectrometry (GC-MS) with use of a modified specific ion selection mode. Benzophenone derivatives were produced by acid hydrolysis of urine samples containing benzodiazepines and (or) their metabolites. We present mass spectra of the newer benzodiazepines--alprazolam, midazolam, and triazolam--and we determined the detection limit (0.2 mg/L) for these drugs as measured with the EMIT d.a.u. benzodiazepine assay and the ETS instrument (both from Syva Co.). We conclude that these algorithms are useful mostly in forensic toxicology in which unequivocal identification of benzodiazepines is the desired goal.

scholarly journals Identification of 19-nor-deoxycorticosterone in normal rat serum by enzyme immunoassay and gas chromatography-mass spectrometry.

1984 ◽  
Vol 31 (1) ◽  
pp. 49-53 ◽  
Author(s):  
YOSHIHARU KOBAYASHI ◽  
TOSHIO OGIHARA ◽  
YAYOI YAMAMURA ◽  
FUKUKO WATANABE ◽  
TOSHIKO KIGUCHI ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Enzyme Immunoassay ◽  
Gas Chromatography Mass Spectrometry ◽  
Rat Serum ◽  
Chromatography Mass Spectrometry ◽  
Normal Rat

Rapid, direct enzyme immunoassay of 11-keto-thromboxane B2 in urine, validated by immunoaffinity/gas chromatography-mass spectrometry

1993 ◽  
Vol 39 (12) ◽  
pp. 2470-2477 ◽  
Author(s):  
R Djurup ◽  
C Chiabrando ◽  
A Jörres ◽  
R Fanelli ◽  
M Foegh ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Enzyme Immunoassay ◽  
Cross Reactivity ◽  
Thromboxane B2 ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Measured Concentration ◽  
Chromatography Mass Spectrometry ◽  
Urine Specimens

Abstract We have developed a direct enzyme immunoassay (EIA) for quantifying immunoreactive 11-keto-thromboxane B2 (iKTXB) in unprocessed human urine. Cross-reactivity with other thromboxane metabolites and prostanoids was negligible. Analytical recovery of 11-keto-TXB2 in urine specimens was 97.4% to 99.8%. Total imprecision for two clinical specimens was 8.5% and 12.2%. Intake of acetylsalicylic acid decreased the measured concentration of iKTXB. Cardiopulmonary bypass, a procedure known to activate platelets, increased the mean excretion rate of iKTXB 10-fold. Simultaneous gas chromatography-mass spectrometry analysis of 11-keto-TXB2 and 11-keto-2,3-dinor TXB2 in urine specimens (n = 17) from healthy subjects indicated that urinary iKTXB concentrations measured by EIA represented a sum of the two 11-keto metabolites. We conclude that the direct EIA is sufficiently sensitive, rapid, simple, and specific to allow screening for alterations in thromboxane biosynthesis in patients.

Quantitative determination of methamphetamine in oral fluid by liquid–liquid extraction and gas chromatography/mass spectrometry

2016 ◽  
Vol 36 (2) ◽  
pp. 195-202 ◽  
Author(s):  
L Bahmanabadi ◽  
M Akhgari ◽  
F Jokar ◽  
HB Sadeghi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Oral Fluid ◽  
Limit Of Detection ◽  
Forensic Toxicology ◽  
Liquid Extraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Liquid Liquid Extraction ◽  
Chromatography Mass Spectrometry ◽  
Limit Of Quantitation

Methamphetamine abuse is one of the most medical and social problems many countries face. In spite of the ban on the use of methamphetamine, it is widely available in Iran’s drug black market. There are many analytical methods for the detection of methamphetamine in biological specimen. Oral fluid has become a popular specimen to test for the presence of methamphetamine. The purpose of the present study was to develop a method for the extraction and detection of methamphetamine in oral fluid samples using liquid–liquid extraction (LLE) and gas chromatography/mass spectrometry (GC/MS) methods. An analytical study was designed in that blank and 50 authentic oral fluid samples were collected to be first extracted by LLE and subsequently analysed by GC/MS. The method was fully validated and showed an excellent intra- and inter-assay precision (reflex sympathetic dystrophy ˂ 10%) for external quality control samples. Recovery with LLE methods was 96%. Limit of detection and limit of quantitation were 5 and 15 ng/mL, respectively. The method showed high selectivity, no additional peak due to interfering substances in samples was observed. The introduced method was sensitive, accurate and precise enough for the extraction of methamphetamine from oral fluid samples in forensic toxicology laboratories.

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