Hormone dependence of a mouse mammary tumor line inducedin vivo by medroxyprogesterone acetate

1990 ◽  
Vol 17 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Edith Kordon ◽  
Claudia Lanari ◽  
Roberto Meiss ◽  
Patricia Elizalde ◽  
Eduardo Charreau ◽  
...  
Keyword(s):  
Mammary Tumor ◽  
Medroxyprogesterone Acetate ◽  
Hormone Dependence ◽  
Tumor Line ◽  
Mouse Mammary Tumor

The effect of DNA-alkylating agents on gene expression from two integrated reporter genes in a mouse mammary tumor line

2002 ◽  
Vol 13 (3) ◽  
pp. 271-280 ◽  
Author(s):  
Michael A Gieseg ◽  
Charley de Bock ◽  
Pamela Turner ◽  
Lynette R Ferguson ◽  
William A Denny
Keyword(s):  
Gene Expression ◽  
Mammary Tumor ◽  
Alkylating Agents ◽  
Reporter Genes ◽  
Tumor Line ◽  
Mouse Mammary Tumor

Isolation of a stromal cell line from an early passage of a mouse mammary tumor line: A model for stromal parenchymal interactions

2004 ◽  
Vol 202 (3) ◽  
pp. 672-682 ◽  
Author(s):  
Caroline A. Lamb ◽  
Victoria Fabris ◽  
Mar�a Alicia Gorostiaga ◽  
Luisa A. Helguero ◽  
Alejo Efeyan ◽  
...  
Keyword(s):  
Cell Line ◽  
Mammary Tumor ◽  
Stromal Cell ◽  
Early Passage ◽  
Tumor Line ◽  
Mouse Mammary Tumor ◽  
Stromal Cell Line

Electron Microscopy of the Nucleic Acid of Mouse Mammary Tumor Virus

1968 ◽  
Vol 26 ◽  
pp. 22-23
Author(s):  
N. H. Sarkar ◽  
Dan H. Moore
Keyword(s):  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Ammonium Acetate ◽  
Dry Weight ◽  
Mouse Tumor ◽  
Mammary Tumor Virus ◽  
Rna Molecules ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
The Subject

Mouse mammary tumor virus (MTV) is believed to contain about 0.8% single stranded ribonucleic acid (RNA). This value of RNA content was estimated on a dry weight basis. The subject of this report is an attempt to visualize the RNA molecules of MTV particles.MTV particles were isolated from RIII mouse (tumor incidence approximately 80%) milk according to the method described by Lyons and Moore. Purified virions from 5 ml of milk were finally suspended in 0.2 ml of PBS, pH 7.4 and was mixed with an equal volume of pronase (5 mg/ml). This mixture was incubated at 37°C for an hour. RNA was extracted three times using freshly prepared cold phenol. It was then treated three times with cold ethyl ether to remove any trace of phenol. The RNA thus extracted was divided into two parts. One part was diluted four fold with 8M urea to avoid aggregation of the molecules. The other part was left untreated. Both samples were then mixed with an equal volume of 1M ammonium acetate, adjusted to pH 8.0 with NH3 containing chymotrypsin at a concentration of 0.01%.

Electron Immunochemical Labeling of Ultrathin Sections of Murine Tumor Viruses and Cell Cultures using the Unlabeled Antibody Enzyme Technique

1976 ◽  
Vol 34 ◽  
pp. 266-267
Author(s):  
W. J. Hamilton
Keyword(s):  
Mammary Tumor ◽  
Leukemia Virus ◽  
Parallel Methods ◽  
Tumor Viruses ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
Antibody Conjugates ◽  
Rauscher Leukemia ◽  
Antigen Localization ◽  
Unlabeled Antibody

The study of RNA tumor viruses has been greatly facilitated by the use of immunochemical tagging methods. In the past these methods have been constrained to antibody conjugates with ferritin or peroxidase. In order to avoid the disadvantages of using conjugated antisera, investigators have applied the unlabeled antibody enzyme method of Sternberger to mammary tumor derived mouse cells prior to embedding for electron microscopy. The current study has successfully applied the Sternberger method to virusproducing cells and purified virus pellets after epoxy-embedding and ultrathin sectioning. The results demonstrate the distinct advantages of this “post-embedding” method for viral antigen localization.Purified Rauscher leukemia virus (RLV) and mouse mammary tumor virus (MMTV) were pelleted, fixed in buffered 2% paraformaldehyde and washed thoroughly. These were dehydrated in acetone, infiltrated and embedded in Spurr resin according to common procedures. A tumor derived cell line, Mm5mt, producing MMTV was embedded by parallel methods.

Detection of Viral Antigens in Mouse and Rat Tumor Cells by Immunoelectron Microscopy Following Periodate-Lysine-Paraformaldehyde (PLP) Fixation

1976 ◽  
Vol 34 ◽  
pp. 252-253
Author(s):  
Y. Ohtsuki ◽  
G. Seman ◽  
J. M. Bowen ◽  
M. Scanlon ◽  
L. Dmochowski
Keyword(s):  
Immunoelectron Microscopy ◽  
Mammary Tumor ◽  
Type A ◽  
Rabbit Serum ◽  
Virus Particles ◽  
Viral Antigens ◽  
Culture Cells ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
Immunoperoxidase Labeling

Recently, periodate-lysine-paraformaldehyde (PLP) fixation was reported for immunoelectron microscopy (1). In PLP fixation, carbohydrates are oxidized by periodate and cross-linked by lysine; paraformaldehyde stabilizes proteins and lipids. By using PLP fixation, intracytoplasmic type A viral antigens have been previously demonstrated by immunoperoxidase labeling (2). In the present study, PLP fixation has been applied for the detection of the same antigens in mouse mammary tumor culture cells by both immunoferritin and immunoperoxidase methods. Rabbit anti-intracytoplasmic type A virus serum (anti-A), kindly provided by Dr. M. Muller (3), rabbit anti-strain A mouse mammary tumor virus (anti-MMTV) and preimmune rabbit serum as control were used to detect viral antigens in cells of C3H/HeJ strain mouse mammary tumor culture. Attempts have been also made to demonstrate peroxidase labeling of type C virus particles in frozen sections of an SD-MSV-induced NZB rat bone tumor tissue by rabbit anti-MuLV serum.

Isolation, Identification, and Assay of [3H]-Porfiromycin Adducts of EMT6 Mouse Mammary Tumor Cell DNA: Effects of Hypoxia and Dicumarol on Adduct Patterns

1991 ◽  
Vol 3 (7) ◽  
pp. 213-223 ◽  
Author(s):  
Maria Tomasz ◽  
Christine S. Hughes ◽  
Dondapati Chowdary ◽  
Susan Riley Keyes ◽  
Roselyn Lipman ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Mammary Tumor ◽  
Mammary Tumor Cell ◽  
Mouse Mammary Tumor ◽  
Mouse Mammary Tumor Cell
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