Isolation of a stromal cell line from an early passage of a mouse mammary tumor line: A model for stromal parenchymal interactions

2004 ◽  
Vol 202 (3) ◽  
pp. 672-682 ◽  
Author(s):  
Caroline A. Lamb ◽  
Victoria Fabris ◽  
Mar�a Alicia Gorostiaga ◽  
Luisa A. Helguero ◽  
Alejo Efeyan ◽  
...  
Keyword(s):  
Cell Line ◽  
Mammary Tumor ◽  
Stromal Cell ◽  
Early Passage ◽  
Tumor Line ◽  
Mouse Mammary Tumor ◽  
Stromal Cell Line

Morphological analysis of the cellular interaction between thymocytes and a thymic stromal cell line (TEL-2)

1991 ◽  
Vol 230 (4) ◽  
pp. 524-530 ◽  
Author(s):  
Kazuho Hirata ◽  
Keiko Mori ◽  
Keiichiro Nakamura ◽  
Masaru Kawabuchi ◽  
Takeshi Watanabe
Keyword(s):  
Cell Line ◽  
Stromal Cell ◽  
Morphological Analysis ◽  
Cellular Interaction ◽  
Stromal Cell Line ◽  
Thymic Stromal Cell

Development and characterization of a human marrow stromal cell line that enhances osteoclast-like cell formation.

Endocrinology ◽  
1995 ◽  
Vol 136 (4) ◽  
pp. 1441-1449 ◽  
Author(s):  
S Takahashi ◽  
S V Reddy ◽  
M Dallas ◽  
R Devlin ◽  
J Y Chou ◽  
...  
Keyword(s):  
Cell Line ◽  
Stromal Cell ◽  
Cell Formation ◽  
Marrow Stromal Cell ◽  
Stromal Cell Line

Hormone dependence of a mouse mammary tumor line inducedin vivo by medroxyprogesterone acetate

1990 ◽  
Vol 17 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Edith Kordon ◽  
Claudia Lanari ◽  
Roberto Meiss ◽  
Patricia Elizalde ◽  
Eduardo Charreau ◽  
...  
Keyword(s):  
Mammary Tumor ◽  
Medroxyprogesterone Acetate ◽  
Hormone Dependence ◽  
Tumor Line ◽  
Mouse Mammary Tumor

scholarly journals Maintenance of high levels of pluripotent hematopoietic stem cells in vitro: effect of stromal cells and c-kit

Blood ◽  
1993 ◽  
Vol 81 (2) ◽  
pp. 365-372 ◽  
Author(s):  
JP Wineman ◽  
S Nishikawa ◽  
CE Muller-Sieburg
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Line ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Hematopoietic Stem ◽  
Stem Cell Maintenance ◽  
Stromal Cell Line ◽  
Cell Maintenance

We show here that mouse pluripotent hematopoietic stem cells can be maintained in vitro on stroma for at least 3 weeks at levels close to those found in bone marrow. The extent of stem cell maintenance is affected by the nature of the stromal cells. The stromal cell line S17 supported stem cells significantly better than heterogeneous, primary stromal layers or the stromal cell line Strofl-1. Stem cells cultured on S17 repopulated all hematopoietic lineages in marrow-ablated hosts for at least 10 months, indicating that this culture system maintained primitive stem cells with extensive proliferative capacity. Furthermore, we demonstrate that, while pluripotent stem cells express c-kit, this receptor appears to play only a minor role in stem cell maintenance in vitro. The addition of an antibody that blocks the interaction of c-kit with its ligand essentially abrogated myelopoiesis in cultures. However, the level of stem cells in antibody-treated cultures was similar to that found in untreated cultures. Thus, it seems likely that the maintenance of primitive stem cells in vitro depends on yet unidentified stromal cell-derived factor(s).

scholarly journals Improved hematopoiesis in anemic Sl/Sld mice by splenectomy and therapeutic transplantation of a hematopoietic microenvironment

Blood ◽  
1989 ◽  
Vol 74 (3) ◽  
pp. 1144-1151
Author(s):  
P Anklesaria ◽  
TJ FitzGerald ◽  
K Kase ◽  
A Ohara ◽  
JS Greenberger
Keyword(s):  
Bone Marrow ◽  
Cell Line ◽  
Hind Limb ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Volume Distribution ◽  
Distribution Analysis ◽  
Marrow Stromal Cell ◽  
Stromal Cell Line ◽  
The Right

The ability of a clonal hematopoiesis-supportive bone-marrow stromal cell line GBlneor to engraft and alter the microenvironment-induced anemia of Sl/Sld mice was studied. Prior to stromal cell transplantation, Sl/Sld mice received 1 Gy total body irradiation (TBI) and 13 Gy to the right hind limb. Two months after intravenous (IV) injection of 5 x 10(5) GBlneor cells, 54.4% +/- 17.0% donor origin (G418r) colony-forming cells were recovered from the right hind limb of Sl/Sld mice. Long-term bone marrow cultures (LTBMCs) established from GBlneor-transplanted mice produced 189.5 CFU-GEMM-forming progenitors/flask over 10 weeks compared with 52.7 +/- 6.2 CFU-GEMM forming progenitors/flask from irradiated nontransplanted Sl/Sld mice. A partial correction of macrocytic anemia was detected 2 months after GBlneor transplantation in splenectomized, irradiated Sl/Sld mice (HgB 7.2 +/- 0.4 g/dL; MCV 68.3 +/- 7.0 fL) compared to splenectomized, irradiated, nontransplanted Sl/Sld mice (HgB 5.5 +/- 1.1 g/dL; MCV 76 +/- 8.5 fL) or control Sl/Sld mice (HgB 5.4 +/- 0.5 g/dL; MCV 82.4 +/- 1.3 fL). Mean RBC volume distribution analysis showed a 2.5-fold increase in percentage of peripheral blood RBCs with MCV less than or equal to 45 fL and confirmed reduction of the MCV in splenectomized- GBlneor-transplanted mice compared to control Sl/Sld mice. A hematopoiesis-suppressive clonal stromal cell line derived from LTBMCs of Sl/Sld mice (Sldneor) engrafted as effectively (43.5% +/- 1.2% G418r CFU-F/limb) as did GBlneor cells (38.3% +/- 0.16% G418r CFU-F/limb) to the irradiated right hind limbs of C57Bl/6 mice. LTBMCs established after 2 or 6 months from Sldneor-transplanted mice showed decreased hematopoiesis (182 +/- 12 [2 months] and 3494.3 +/- 408.1 [6 months] CFU-GEMM forming progenitors/flask over 10 weeks) compared to those established from GBlneor-transplanted mice (5980 +/- 530 [2 months] and 7728 +/- 607, [6 months] CFU-GEMM progenitors forming/flask). Thus, transplantation of clonal bone-marrow stromal cell lines in vivo can stably transfer their physiologic properties to normal or mutant mice.

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