Expression of L-PHA-binding proteins in breast cancer: Reconstitution and molecular characterization of β1–6 branched oligosaccharides in three-dimensional cell culture

1996 ◽  
Vol 38 (3) ◽  
pp. 313-324 ◽  
Author(s):  
Yoshinori Taeda ◽  
Masato Nose ◽  
Sen Hiraizumi ◽  
Noriaki Ohuchi
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Molecular Characterization ◽  
Binding Proteins ◽  
Three Dimensional ◽  
Dimensional Cell

scholarly journals Modelling molecular mechanisms of breast cancer and invasion: lessons from the normal gland

2007 ◽  
Vol 35 (1) ◽  
pp. 18-22 ◽  
Author(s):  
M.J. Bissell
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Molecular Mechanisms ◽  
Three Dimensional ◽  
Cell Types ◽  
Genes And Environment ◽  
Different Cell Types ◽  
Cancerous Cells ◽  
Cancer Studies ◽  
Dimensional Cell

The interplay between genes and environment is complex, particularly when it comes to cancer. Studies on breast cancer cells have shown that environmental influences dominate over genotype in their effect on phenotype, and can cause cancerous cells to revert to a non-malignant phenotype, while remaining genotypically malignant. Using breast tissue in three-dimensional cell culture has proved a better model than traditional two-dimensional cell culture in that different cell types can be seen to behave differently to the same pro­-apoptotic signal, with normal cells surviving.

scholarly journals Characterization of an Aggregated Three-Dimensional Cell Culture Model by Multimodal Mass Spectrometry Imaging

2020 ◽  
Vol 92 (18) ◽  
pp. 12538-12547 ◽  
Author(s):  
Lucy E. Flint ◽  
Gregory Hamm ◽  
Joseph D. Ready ◽  
Stephanie Ling ◽  
Catherine J. Duckett ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Culture ◽  
Mass Spectrometry Imaging ◽  
Three Dimensional ◽  
Cell Culture Model ◽  
Culture Model ◽  
Dimensional Cell

scholarly journals Fabrication and Characterization of Magnetic Microrobots for Three‐Dimensional Cell Culture and Targeted Transportation

2013 ◽  
Vol 25 (41) ◽  
pp. 5863-5868 ◽  
Author(s):  
Sangwon Kim ◽  
Famin Qiu ◽  
Samhwan Kim ◽  
Ali Ghanbari ◽  
Cheil Moon ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Fabrication And Characterization ◽  
Magnetic Microrobots ◽  
Dimensional Cell

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

A novel fluffy hydroxylapatite fiber scaffold with deep interconnected pores designed for three-dimensional cell culture

2014 ◽  
Vol 2 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Lin Jin ◽  
Zhang-Qi Feng ◽  
Ting Wang ◽  
Zhuozhuo Ren ◽  
Shuangshuang Ma ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Interconnected Pores ◽  
Dimensional Cell
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